Chondroprotective Effects of Chondrogenic Differentiated Adipose-Derived Mesenchymal Stem Cells Sheet on Degenerated Articular Cartilage in an Experimental Rabbit Model

Adipose-derived stem cells (ADSCs) have been studied for many years as a therapeutic option for osteoarthritis (OA); however, their efficacy remains insufficient. Since platelet-rich plasma (PRP) induces chondrogenic differentiation in ADSCs and the formation of a sheet structure by ascorbic acid can increase the number of viable cells, we hypothesized that the injection of chondrogenic cell sheets combined with the effects of PRP and ascorbic acid may hinder the progression of OA. The effects of induction of differentiation by PRP and formation of sheet structure by ascorbic acid on changes in chondrocyte markers (collagen II, aggrecan, Sox9) in ADSCs were evaluated. Changes in mucopolysaccharide and VEGF-A secretion from cells injected intra-articularly in a rabbit OA model were also evaluated. ADSCs treated by PRP strongly chondrocyte markers, including type II collagen, Sox9, and aggrecan, and their gene expression was maintained even after sheet-like structure formation induced by ascorbic acid. In this rabbit OA model study, the inhibition of OA progression by intra-articular injection was improved by inducing chondrocyte differentiation with PRP and sheet structure formation with ascorbic acid in ADSCs.

[1]  X. Miao,et al.  Effect of Amniotic Membrane/Collagen-Based Scaffolds on the Chondrogenic Differentiation of Adipose-Derived Stem Cells and Cartilage Repair , 2021, Frontiers in Cell and Developmental Biology.

[2]  Jiazhu Tang,et al.  Platelet-rich plasma versus hyaluronic acid in the treatment of knee osteoarthritis: a meta-analysis , 2020, Journal of Orthopaedic Surgery and Research.

[3]  H. Tsuchiya,et al.  Periodic injections of adipose-derived stem cell sheets attenuate osteoarthritis progression in an experimental rabbit model , 2020, BMC Musculoskeletal Disorders.

[4]  L. Rong,et al.  Bone marrow mesenchymal stem cell-derived exosomes protect cartilage damage and relieve knee osteoarthritis pain in a rat model of osteoarthritis , 2020, Stem Cell Research & Therapy.

[5]  A. Gelber,et al.  Osteoarthritis , 2020, Annals of Internal Medicine.

[6]  H. Judawisastra,et al.  Chondrogenic differentiation of adipose-derived mesenchymal stem cells induced by L-ascorbic acid and platelet rich plasma on silk fibroin scaffold , 2018, PeerJ.

[7]  E. Jang,et al.  Comparative effectiveness of oral pharmacologic interventions for knee osteoarthritis: A network meta-analysis , 2018, Modern rheumatology.

[8]  A. Kaneko,et al.  Steroid therapy and renal dysfunction are independently associated with serum levels of matrix metalloproteinase-3 in patients with rheumatoid arthritis , 2018, Modern rheumatology.

[9]  V. Tieppo Francio,et al.  Oral Versus Topical Diclofenac Sodium in the Treatment of Osteoarthritis , 2017, Journal of pain & palliative care pharmacotherapy.

[10]  K. Endo,et al.  Limited efficacy of COX-2 inhibitors on nerve growth factor and metalloproteinases expressions in human synovial fibroblasts. , 2016, Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association.

[11]  J. Driban,et al.  Objectively Measured Physical Activity and Symptoms Change in Knee Osteoarthritis. , 2016, The American journal of medicine.

[12]  Lihui Weng,et al.  Intra-articular treatment of knee osteoarthritis: from anti-inflammatories to products of regenerative medicine , 2016, The Physician and sportsmedicine.

[13]  Xiang Wang,et al.  Transforming growth factor-β1 induces type II collagen and aggrecan expression via activation of extracellular signal-regulated kinase 1/2 and Smad2/3 signaling pathways. , 2015, Molecular medicine reports.

[14]  N. Sugimoto,et al.  The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression , 2015, BMC Musculoskeletal Disorders.

[15]  Jian Wang,et al.  Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro. , 2015, Experimental and therapeutic medicine.

[16]  Wei‐ping Chen,et al.  Astaxanthin ameliorates cartilage damage in experimental osteoarthritis , 2015, Modern rheumatology.

[17]  Troy D. Bornes,et al.  Mesenchymal stem cells in the treatment of traumatic articular cartilage defects: a comprehensive review , 2014, Arthritis Research & Therapy.

[18]  M. Fini,et al.  Adipose-derived mesenchymal stem cells for cartilage tissue engineering: state-of-the-art in in vivo studies. , 2014, Journal of biomedical materials research. Part A.

[19]  F. Gozzo,et al.  Cell kinetics, DNA integrity, differentiation, and lipid fingerprinting analysis of rabbit adipose-derived stem cells , 2014, In Vitro Cellular & Developmental Biology - Animal.

[20]  J. Hui,et al.  Advances in Mesenchymal Stem Cell-based Strategies for Cartilage Repair and Regeneration , 2014, Stem Cell Reviews and Reports.

[21]  T. Kamarul,et al.  Advances of human bone marrow-derived mesenchymal stem cells in the treatment of cartilage defects: A systematic review , 2014, Experimental biology and medicine.

[22]  G. Im,et al.  In vitro chondrogenesis and in vivo repair of osteochondral defect with human induced pluripotent stem cells. , 2014, Biomaterials.

[23]  H. Potter,et al.  Restoration of articular cartilage. , 2014, The Journal of bone and joint surgery. American volume.

[24]  H. Tsuchiya,et al.  A Novel Method to Apply Osteogenic Potential of Adipose Derived Stem Cells in Orthopaedic Surgery , 2014, PloS one.

[25]  T. D. Nguyen,et al.  Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage , 2013, Stem Cell Research & Therapy.

[26]  R. Giardino,et al.  Intra-articular delivery of adipose derived stromal cells attenuates osteoarthritis progression in an experimental rabbit model , 2013, Arthritis Research & Therapy.

[27]  H. Madry,et al.  Mesenchymal stem cells for the treatment of cartilage lesions: from preclinical findings to clinical application in orthopaedics , 2013, Knee Surgery, Sports Traumatology, Arthroscopy.

[28]  K. Berend,et al.  Complications of Total Knee Arthroplasty: Standardized List and Definitions of The Knee Society , 2013, Clinical orthopaedics and related research.

[29]  Changqing Zhang,et al.  Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration. , 2012, Biomaterials.

[30]  B. Boyan,et al.  Adipose stem cells can secrete angiogenic factors that inhibit hyaline cartilage regeneration , 2012, Stem Cell Research & Therapy.

[31]  K. Chua,et al.  Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation , 2012, Clinics.

[32]  J. Toguchida,et al.  Prostaglandin E2 receptor type 2-selective agonist prevents the degeneration of articular cartilage in rabbit knees with traumatic instability , 2011, Arthritis research & therapy.

[33]  I. Sekiya,et al.  Intra‐articular injections of bone morphogenetic protein‐7 retard progression of existing cartilage degeneration , 2010, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[34]  S. Omokawa,et al.  Cell sheet injection as a technique of osteogenic supply. , 2010, International journal of stem cells.

[35]  Raveendhara R. Bannuru,et al.  OARSI recommendations for the management of hip and knee osteoarthritis: the semantics of differences and changes. , 2010, Osteoarthritis and cartilage.

[36]  Weitao Jia,et al.  The prophylactic effects of platelet-leucocyte gel in osteomyelitis: an experimental study in a rabbit model. , 2010, The Journal of bone and joint surgery. British volume.

[37]  Y. Maugars,et al.  From osteoarthritis treatments to future regenerative therapies for cartilage. , 2009, Drug discovery today.

[38]  S. Goldring,et al.  Osteoarthritis , 2007 .

[39]  S. Jimenez,et al.  Osteoarthritis cartilage histopathology: grading and staging. , 2006, Osteoarthritis and cartilage.

[40]  M. Hedrick,et al.  Multipotential differentiation of adipose tissue-derived stem cells. , 2005, The Keio journal of medicine.

[41]  V. Harde,et al.  Vascular endothelial growth factor (VEGF) induces matrix metalloproteinase expression in immortalized chondrocytes , 2004, The Journal of pathology.

[42]  Frank P Barry,et al.  Stem cell therapy in a caprine model of osteoarthritis. , 2003, Arthritis and rheumatism.

[43]  T. Kawamoto,et al.  Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues, whole-animals, insects and plants. , 2003, Archives of histology and cytology.

[44]  M. Saito,et al.  Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair of cartilage defects in osteoarthritic knees. , 2002, Osteoarthritis and cartilage.

[45]  C. Rorabeck,et al.  Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage. , 1997, The Journal of clinical investigation.

[46]  D Amiel,et al.  Characterization of a model of osteoarthritis in the rabbit knee. , 1996, Osteoarthritis and cartilage.

[47]  Joseph M. Mansour,et al.  Mesenchymal Cell-Based Repair of Large Full Thickness Defects of Articular Cartilage , 1994 .

[48]  G. Ville,et al.  Histological cartilage changes in a rabbit model of osteoarthritis. , 1987, The Journal of rheumatology.

[49]  S. Pinnell,et al.  Regulation of collagen synthesis by ascorbic acid. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[50]  Katsuhiro Hayashi,et al.  Uncultured adipose-derived regenerative cells promote peripheral nerve regeneration , 2013, Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association.

[51]  Yun-song Zhang,et al.  [Effect of platelet-rich plasma on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro]. , 2011, Nan fang yi ke da xue xue bao = Journal of Southern Medical University.

[52]  Z. Alfonso,et al.  Adipose-derived stem cells. , 2008, Methods in molecular biology.

[53]  Yasuhiko Tabata,et al.  The therapeutic effects of basic fibroblast growth factor contained in gelatin hydrogel microspheres on experimental osteoarthritis in the rabbit knee. , 2006, Arthritis and rheumatism.