Occurrence of Viruses in US Groundwaters

US groundwater may be subjected to fecal contamination from a variety of sources. This study sought to develop a preliminary assessment database on virus occurrence in groundwater systems at the national level. Information on physical and geological characteristics of groundwater wells, along with various microbial and physicochemical water quality parameters, was collected, and possible correlation with the presence of human viruses was investigated. Groundwater samples from 448 sites in 35 states were collected and assayed for microorganisms and chemical contaminants. Infective viruses, viral nucleic acid, bacteriophages, and bacteria were present in 4.8, 31.5, 20.7, and 15.1% of samples, respectively. Statistical analysis showed that one-time sampling is not sufficient for proper risk characterization. No significant direct correlations existed between the presence of virus and microbial indicators. However, when only the sites with repeat sampling were examined for correlations between indicators and pathogens, it was observed that if a site tested positive for a microbial indicator, it also tested positive at some point in time for pathogens.

[1]  C. Gerba,et al.  Bacteriophage Transport in Sandy Soil and Fractured Tuff , 1989, Applied and environmental microbiology.

[2]  Charles P. Gerba,et al.  Viruses in a community water supply associated with an outbreak of gastroenteritis and infectious hepatitis , 1982 .

[3]  M. Gross,et al.  Virus Removal by Sand Filtration of Septic Tank Effluent , 1990 .

[4]  L. Anderson,et al.  Temporal and geographic patterns of isolates of nonpolio enterovirus in the United States, 1970-1983. , 1986, The Journal of infectious diseases.

[5]  Charles P. Gerba,et al.  Fate of Wastewater Bacteria and Viruses in Soil , 1975 .

[6]  M. Lechevallier,et al.  A Strategy for Detection of Viruses in Groundwater by PCR , 1999, Applied and Environmental Microbiology.

[7]  C. Gerba,et al.  Detection of enteroviruses in groundwater with the polymerase chain reaction , 1993, Applied and environmental microbiology.

[8]  A H Havelaar,et al.  F-specific RNA bacteriophages are adequate model organisms for enteric viruses in fresh water , 1993, Applied and environmental microbiology.

[9]  M. Estes,et al.  Serotype variation of human group A rotaviruses in two regions of the USA. , 1990, The Journal of infectious diseases.

[10]  R. Holman,et al.  Visualizing geographic and temporal trends in rotavirus activity in the United States, 1991 to 1996. National Respiratory and Enteric Virus Surveillance System Collaborating Laboratories. , 1997, The Pediatric infectious disease journal.

[11]  K. Schwab,et al.  A Simple Membrane Filter Method to Concentrate and Enumerate Male‐Specific RNA Coliphages , 1990 .

[12]  C. Gerba,et al.  Viruses in groundwater beneath sewage irrigated cropland , 1984 .

[13]  C. Gerba,et al.  The Use of Microorganisms as Ground‐Water Tracers: A Review , 1982 .

[14]  R. Glass,et al.  Serotypes and electropherotypes of human rotavirus in the USA: 1987-1989. , 1990, The Journal of infectious diseases.

[15]  F. M. Wellings,et al.  Demonstration of Virus in Groundwater after Effluent Discharge onto Soil , 1975, Applied microbiology.