Flexible and elastic scaffolds for cartilage tissue engineering prepared by stereolithography using poly(trimethylene carbonate)-based resins.

The aim of this study is to investigate the applicability of flexible and elastic poly(trimethylene carbonate) (PTMC) structures prepared by stereolithography as scaffolds for cartilage tissue engineering. A three-armed methacrylated PTMC macromer with a molecular weight of 3100 g mol(-1) is used to build designed scaffolds with a pore diameter of 350 ± 12 μm and a porosity of 54.0 ± 2.2%. Upon seeding of bovine chondrocytes in the scaffolds, the cells adhere and spread on the PTMC surface. After culturing for 6 weeks, also cells with a round morphology are present, indicative of the differentiated chondrocyte phenotype. Sulphated glycosaminoglycans and fibrillar collagens are deposited by the cells. During culturing for 6 weeks, the compression moduli of the constructs increases 50% to approximately 100 kPa.

[1]  J. Mansour,et al.  Biomechanics of cartilage , 2013 .

[2]  J. Feijen,et al.  Flexible, elastic and tear-resistant networks prepared by photo-crosslinking poly(trimethylene carbonate) macromers. , 2012, Acta biomaterialia.

[3]  Dong-Woo Cho,et al.  Solid freeform fabrication technology applied to tissue engineering with various biomaterials , 2012 .

[4]  Jan Feijen,et al.  Preparation of flexible and elastic poly(trimethylene carbonate) structures by stereolithography. , 2011, Macromolecular bioscience.

[5]  J. Feijen,et al.  Dynamic culturing of smooth muscle cells in tubular poly(trimethylene carbonate) scaffolds for vascular tissue engineering. , 2011, Tissue engineering. Part A.

[6]  J. Feijen,et al.  Flexible scaffolds based on poly(trimethylene carbonate) networks for cardiac tissue engineering. , 2010, Journal of controlled release : official journal of the Controlled Release Society.

[7]  Clemens A van Blitterswijk,et al.  Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing. , 2010, Acta biomaterialia.

[8]  Mary Murphy,et al.  Type II collagen-hyaluronan hydrogel--a step towards a scaffold for intervertebral disc tissue engineering. , 2010, European cells & materials.

[9]  Katia Bertoldi,et al.  Mathematically defined tissue engineering scaffold architectures prepared by stereolithography. , 2010, Biomaterials.

[10]  C A van Blitterswijk,et al.  Synthesis and characterization of hyaluronic acid-poly(ethylene glycol) hydrogels via Michael addition: An injectable biomaterial for cartilage repair. , 2010, Acta biomaterialia.

[11]  Zhiyuan Zhong,et al.  Enzymatically crosslinked dextran-tyramine hydrogels as injectable scaffolds for cartilage tissue engineering. , 2010, Tissue engineering. Part A.

[12]  C A van Blitterswijk,et al.  Enzymatically-crosslinked injectable hydrogels based on biomimetic dextran-hyaluronic acid conjugates for cartilage tissue engineering. , 2010, Biomaterials.

[13]  F. Boschetti,et al.  Effect of in vitro culture on a chondrocyte-fibrin glue hydrogel for cartilage repair , 2010, Knee Surgery, Sports Traumatology, Arthroscopy.

[14]  Jan Feijen,et al.  A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography. , 2009, Biomaterials.

[15]  Ta-Jen Huang,et al.  Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering. , 2009, Acta biomaterialia.

[16]  Jan Feijen,et al.  Fumaric acid monoethyl ester-functionalized poly(D,L-lactide)/N-vinyl-2-pyrrolidone resins for the preparation of tissue engineering scaffolds by stereolithography. , 2009, Biomacromolecules.

[17]  B. Amsden,et al.  The role of oxidation and enzymatic hydrolysis on the in vivo degradation of trimethylene carbonate based photocrosslinkable elastomers. , 2009, Biomaterials.

[18]  J. Feijen,et al.  Trimethylene carbonate and epsilon-caprolactone based (co)polymer networks: mechanical properties and enzymatic degradation. , 2008, Biomacromolecules.

[19]  Minna Kellomäki,et al.  A review of rapid prototyping techniques for tissue engineering purposes , 2008, Annals of medicine.

[20]  Jason A Burdick,et al.  Review: photopolymerizable and degradable biomaterials for tissue engineering applications. , 2007, Tissue engineering.

[21]  Clemens A van Blitterswijk,et al.  Modulation of chondrocyte phenotype for tissue engineering by designing the biologic-polymer carrier interface. , 2006, Biomacromolecules.

[22]  I. Martin,et al.  The regulation of expanded human nasal chondrocyte re-differentiation capacity by substrate composition and gas plasma surface modification. , 2006, Biomaterials.

[23]  S. Hollister.  Porous scaffold design for tissue engineering , 2005, Nature materials.

[24]  A. U. Daniels,et al.  Effects of scaffold composition and architecture on human nasal chondrocyte redifferentiation and cartilaginous matrix deposition. , 2005, Biomaterials.

[25]  Thomas Boland,et al.  Rapid prototyping of tissue-engineering constructs, using photopolymerizable hydrogels and stereolithography. , 2004, Tissue engineering.

[26]  Mary B Chan-Park,et al.  Novel photopolymerizable biodegradable triblock polymers for tissue engineering scaffolds: synthesis and characterization. , 2004, Macromolecular bioscience.

[27]  E. Sachlos,et al.  Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. , 2003, European cells & materials.

[28]  J. E. Mark,et al.  EFFECTS OF DILUTION DURING CROSSLINKING ON STRAIN-INDUCED CRYSTALLIZATION IN CIS -1,4-POLYISOPRENE NETWORKS. I. EXPERIMENTAL RESULTS , 2002 .

[29]  P. Rüegsegger,et al.  A new method for the model‐independent assessment of thickness in three‐dimensional images , 1997 .