Comparisons of Cornea Cold, a New Corneal Storage Medium, and Optisol-GS.

PURPOSE This study compares the quality of donor corneal tissue stored in Optisol-GS and Cornea Cold. METHODS Seventeen pairs of donor corneas were obtained from an eye bank. One of each pair was stored in Cornea Cold or Optisol-GS. Endothelial cell loss (ECL), central corneal thickness (CCT), and endothelial cell density (ECD) were measured at 7 and 21 days of storage. Qualitative metrics were evaluated by using a slit lamp. RESULTS At days 7 and 21, there were no observed differences in qualitative corneal health of the samples. There were no statistical differences in the mean ECL at 7 and 21 days between the 2 groups (P = 0.07 and P = 0.50, respectively). At 7 days, the mean CCT was 644 ± 52 μm in the Cornea Cold group and 591 ± 64 μm in the Optisol-GS group (P = 0.001). At 21 days, CCT was 714 ± 55 μm in the Cornea Cold group and 708 ± 58 μm in the Optisol-GS group (P = 0.70). The mean ECD was not statistically different between the groups (P = 0.56 at 7 days and P = 0.14 at 21 days). CONCLUSIONS Storage of corneal donor tissue in the Optisol-GS and Cornea Cold storage media resulted in statistically comparable ECL and ECD for up to 21 days. CCT was higher in Cornea Cold at 7 days, but this discrepancy disappeared at 21 days.

[1]  Jameson Clover.  Slit-Lamp Biomicroscopy. , 2018, Cornea.

[2]  I. Dapena,et al.  Improving the success rate of human corneal endothelial cell cultures from single donor corneas with stabilization medium , 2017, Cell and Tissue Banking.

[3]  N. V. Prajna,et al.  A Prospective, In Vitro, Randomized Study to Compare Two Media for Donor Corneal Storage , 2016, Cornea.

[4]  B. Jeng,et al.  Preservation of the Corneal Epithelium in Different Corneal Storage Media , 2015, Cornea.

[5]  S. Debanne,et al.  Randomized, Prospective, Single-Masked Clinical Trial of Endothelial Keratoplasty Performance With 2 Donor Cornea 4°C Storage Solutions and Associated Chambers , 2015, Cornea.

[6]  C. Monnereau,et al.  Dehydration of corneal anterior donor tissue with polyethylene glycol (PEG)-enriched media , 2014, Cell and Tissue Banking.

[7]  F. Javadi,et al.  Comparing quantitative and qualitative indices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C , 2014, Cell and Tissue Banking.

[8]  L. Drolsum,et al.  Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan , 2013, Acta ophthalmologica.

[9]  C. Wetzel,et al.  Quality assessment of corneal storage media and their components , 2013, Graefe's Archive for Clinical and Experimental Ophthalmology.

[10]  Anoop Nagwani,et al.  Storage , 2010, Web Operations.

[11]  V. Smith,et al.  Evaluation of Megacell™ MEM as a Storage Medium for Corneas Destined for Transplantation , 2009, Ophthalmic Research.

[12]  Leo J. Maguire,et al.  Comparison of Chen Medium and Optisol-GS for Human Corneal Preservation at 4°C: Results of Transplantation , 2001 .

[13]  D. Hodge,et al.  In Vitro Comparison of Chen Medium and Optisol-GS Medium for Human Corneal Storage , 2000, Cornea.

[14]  H. Grossniklaus,et al.  The corneal epithelium after optisol-GS storage. , 1996, Cornea.

[15]  M. Lynn,et al.  Viability of human corneal endothelium following Optisol-GS storage. , 1995, Archives of ophthalmology.