Serum protein fractionation by capillary electrophoresis and its clinical application

Serum protein fractionation using capillary zone electrophoresis and the application is described. There is 20-30 major components in human serum protein, even though the several hundred proteins are exist. However, in serum protein fractionation by cellulose acetate membrane electrophoresis applied to the clinically, it is only five fractions which intermingle many proteins ingredients with one fraction. Clinically, combining change of the 5 fractions has been made to be the assistance of the diagnosis. In the meantime, capillary zone electrophoresis for excellent separation can be consequentially divided the serum proteins into 10 fractions. However, it becomes a result of being inconvenient on the contrary. The clinical significance equal to conventional electrophoresis of protein would be found when divided protein fractions are specially integrated for 5 fractions. The purpose was achieved by the CZE 2000 instrument (Beckman-Coulter Co., USA) and was obtained by the preparation of the software which arranges the serum protein fractions are originally divided into 10 peaks for 5 fractionation. Therefore, it is possible that can fundamentally follow clinical significance by cellulose acetate membrane electrophoresis and moreover, the data of 10 fractionation is also taken out. And, for the identification of regarded M-protein in cases of multiple myeloma and MGUS (monoclonal gammopathy undetermined significance), it is conveniently possible by subtraction method using the capillary electrophoresis. There is dissociation of α1 fraction value between cellulose acetate membrane electrophoresis and capillary electrophoresis. From our detailed experimental results, it is proved that is due to destaining of α1-acid glycoprotein.