Identification of lactic acid bacterial strains with high conjugated linoleic acid-producing ability from natural sauerkraut fermentations.

Natural sauerkraut fermentations contain a great number of lactic acid bacteria. The aim of this study was to identify lactic acid bacterial strains with high conjugated linoleic acid (CLA)-producing ability from natural sauerkraut fermentations. Fifteen CLA-producing lactic acid bacterial strains were isolated in the study. One of these strains, designated as NCUL005, showed the highest CLA-producing ability (0.623 mg/mL). The transformation efficiency of converting linoleic acid into CLA by NCUL005 was 26.67%. The CLA produced by NCUL005 comprised a mixture of 32.2% cis9, trans11-C18:2 isomer and 67.8% trans10, cis12-C18:2 isomer. NCUL005 was identified as Lactobacillus plantarum, based on its cell morphology, characteristics of lactic acid production, and analysis result from Biolog Microbial Identification System (BMIS).

[1]  G. Kaban,et al.  Identification of lactic acid bacteria and Gram-positive catalase-positive cocci isolated from naturally fermented sausage (sucuk). , 2008, Journal of food science.

[2]  H. P. Fleming,et al.  DNA Fingerprinting of Lactic Acid Bacteria in Sauerkraut Fermentations , 2007, Applied and Environmental Microbiology.

[3]  E. Drosinos,et al.  Phenotypic and technological diversity of lactic acid bacteria and staphylococci isolated from traditionally fermented sausages in southern Greece. , 2007, Food microbiology.

[4]  S. González,et al.  Conjugated linoleic acid conversion by dairy bacteria cultured in MRS broth and buffalo milk , 2007, Letters in applied microbiology.

[5]  T. Lin,et al.  Conjugated linoleic acid production by immobilized cells of Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus acidophilus , 2005 .

[6]  M. Mataragas,et al.  Characterization of the microbial flora from a traditional Greek fermented sausage. , 2005, Meat science.

[7]  A. Palva,et al.  Screening of lactic acid bacteria from fermented vegetables by carbohydrate profiling and PCR–ELISA , 2004, Letters in applied microbiology.

[8]  H. P. Fleming,et al.  Malolactic Activity of Lactic Acid Bacteria during Sauerkraut Fermentation , 2004 .

[9]  S. Kishino,et al.  Conjugated linoleic acid production from castor oil by Lactobacillus plantarum JCM 1551 , 2004 .

[10]  D. Oh,et al.  Bioconversion of linoleic acid into conjugated linoleic acid during fermentation and by washed cells of Lactobacillus reuteri , 2003, Biotechnology Letters.

[11]  S. Gilliland,et al.  Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin. , 2003, Journal of dairy science.

[12]  H. P. Fleming,et al.  Bacteriophage Ecology in Commercial Sauerkraut Fermentations , 2003, Applied and Environmental Microbiology.

[13]  J. A. Kelsey,et al.  Analysis of variation in cis-9, trans-11 conjugated linoleic acid (CLA) in milk fat of dairy cows. , 2002, Journal of dairy science.

[14]  R. Liu,et al.  Increase of conjugated linoleic acid content in milk by fermentation with lactic acid bacteria , 2002 .

[15]  H. P. Fleming,et al.  Identification and Characterization of Leuconostoc fallax Strains Isolated from an Industrial Sauerkraut Fermentation , 2002, Applied and Environmental Microbiology.

[16]  T. Lin,et al.  Linoleic acid Isomerase Activity in Enzyme Extracts from Lactobacillus Acidophilus and Propionibacterium Freudenreichii ssp. Shermanii , 2002 .

[17]  M. Cook,et al.  The biologically active isomers of conjugated linoleic acid. , 2001, Progress in lipid research.

[18]  S. Kishino,et al.  Conjugated Linoleic Acid Accumulation via 10-Hydroxy-12-Octadecaenoic Acid during Microaerobic Transformation of Linoleic Acid by Lactobacillus acidophilus , 2001, Applied and Environmental Microbiology.

[19]  M. Tokach,et al.  Conjugated linoleic acid , 2000, Animal Health Research Reviews.

[20]  H. Steinhart,et al.  The potential anticarcinogenic conjugated linoleic acid, cis-9,trans-11 C18:2, in milk of different species: Cow, goat, ewe, sow, mare, woman , 1999 .

[21]  T. Lin,et al.  Conjugated linoleic acid concentration as affected by lactic cultures and added linoleic acid , 1999 .

[22]  Björck,et al.  Production of conjugated linoleic acid by dairy starter cultures , 1998, Journal of applied microbiology.

[23]  C. S. Pederson,et al.  Bulletin: Number 824: The Sauerkraut Fermentation , 1969 .

[24]  S. B. Tove,et al.  Biohydrogenation of Unsaturated Fatty Acids III. PURIFICATION AND PROPERTIES OF A LINOLEATE Δ12-CIS,Δ11-TRANS-ISOMERASE FROM BUTYRIVIBRIO FIBRISOLVENS , 1967 .

[25]  Wu Gui-ying Study on the Process Conditions of Transformation Linoleic Acid into Conjugated Linoleic Acid Via Lactobacillus Acidophilus , 2006 .

[26]  S. B. Tove,et al.  Biohydrogenation of Unsaturated Fatty Acids , 2003 .

[27]  Chen Chao-yin Linoleate isomerase and its properties , 2002 .