Interference reflection microscopic study of sites of association between gliding bacteria and glass substrata

Sites of close contact between gliding Cytophaga sp. strain U67 cells and glass were examined by interference reflection microscopy. Site patterns changed during translocation and moved relative to the substratum, in contrast to previous interference reflection microscopy observations of fibroblast and amoeboid motility. Sinistral rotation around the long axis of the cell was coupled with gliding, except when curved cells traversed curvilinear pathways. Close contact was temporary, since cells flipped up off the substratum on one pole, pivoted, or were displaced laterally in collisions. Other members of the order Cytophagales and Myxococcus sp. demonstrated similar patterns of close association with substrata.

[1]  D. Gingell,et al.  Contacts of chick fibroblasts on glass: results and limitations of quantitative interferometry. , 1988, Journal of cell science.

[2]  M. Fletcher.  Attachment of Pseudomonas fluorescens to glass and influence of electrolytes on bacterium-substratum separation distance , 1988, Journal of bacteriology.

[3]  J. Pate,et al.  Gliding motility in procaryotic cells , 1988 .

[4]  R. Lewin,et al.  Characterization of gliding motility in Flexibacter polymorphus. , 1988, Cell motility and the cytoskeleton.

[5]  K. Kawai,et al.  Characteristics of Gliding Bacteria Isolated from Diseased Cultured Flounder,Paralichthys olivaceous , 1986 .

[6]  R. P. Burchard,et al.  Guidance of Cytophaga sp. strain U67 gliding on the sheaths of Oscillatoria princeps , 1985, Journal of bacteriology.

[7]  M. Rosenberg.  Bacterial adherence to hydrocarbons: a useful technique for studying cell surface hydrophobicity , 1984 .

[8]  P. Rutter,et al.  Physicochemical Interactions of the Substratum, Microorganisms, and the Fluid Phase , 1984 .

[9]  Robert P. Burchard,et al.  Gliding Motility and Taxes , 1984 .

[10]  N. G. Carr,et al.  The Biology of Cyanobacteria , 1982 .

[11]  H. Berg,et al.  Gliding motility of Cytophaga sp. strain U67 , 1982, Journal of bacteriology.

[12]  H. Reichenbach.  Taxonomy of the gliding bacteria. , 1981, Annual review of microbiology.

[13]  R P Burchard,et al.  Gliding motility of prokaryotes: ultrastructure, physiology, and genetics. , 1981, Annual review of microbiology.

[14]  D. Kaiser,et al.  Social gliding is correlated with the presence of pili in Myxococcus xanthus. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[15]  D. Gingell,et al.  Interference reflection microscopy. A quantitative theory for image interpretation and its application to cell-substratum separation measurement. , 1979, Biophysical journal.

[16]  T. Preston,et al.  An experimental study of the interaction between the soil amoeba Naegleria gruberi and a glass substrate during amoeboid locomotion. , 1978, Journal of cell science.

[17]  W. Strohl,et al.  Cytophaga aquatilis sp. nov., a Facultative Anaerobe Isolated from the Gills of Freshwater Fish , 1978 .

[18]  T. MacRae,et al.  Evidence for motility-related fimbriae in the gliding microorganism Myxococcus xanthus. , 1976, Canadian journal of microbiology.

[19]  C. S. Izzard,et al.  Cell-to-substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique. , 1976, Journal of cell science.

[20]  M. Abercrombie,et al.  Adhesions of fibroblasts to substratum during contact inhibition observed by interference reflection microscopy. , 1975, Experimental cell research.

[21]  R. Lewin,et al.  Goblet-shaped Sub-units from the Wall of a Marine Gliding Microbe , 1973 .

[22]  J. Henrichsen,et al.  Bacterial surface translocation: a survey and a classification. , 1972, Bacteriological reviews.

[23]  R. Castenholz,et al.  GLIDING MOTILITY IN THE BLUE‐GREEN ALGA OSCILLATORIA PRINCEPS1 , 1971 .

[24]  W. Chilton,et al.  Galactosamine Glycan of Chondrococcus columnaris , 1966, Science.

[25]  A. S. G. Curtis,et al.  THE MECHANISM OF ADHESION OF CELLS TO GLASS , 1964, The Journal of cell biology.

[26]  R. R. Rucker,et al.  Pathogenic Myxobacteria , 1944 .