Short‐term storage of salmonids semen in a sodium alginate‐based extender

Short‐term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our study was to analyse the effect of a sodium alginate‐based extender on sperm function in the short‐term storage of salmonids semen. Samples of Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss were stored in Storfish® (Ext‐C) and Storfish® supplemented with sodium alginate (Ext‐A) during 10 days at 4°C. After storage, motility, viability, mitochondrial membrane potential (ΔΨmit), superoxide anion (O2−) level and DNA fragmentation (DNA Frag) were assessed. Ext‐A had positive effect in preservation of sperm motility, viability, ΔΨmit, O2− level and DNA integrity in the three species analysed compared to control samples. In Ext‐A, the spermatozoa of S. salar and O. mykiss showed significantly higher motility, viability and ΔΨmit than O. kisutch. However, O. kisutch and O. mykiss had significantly lower O2− level than S. salar, and DNA fragmentation in O. kisutch and S. salar was significantly lower than in samples of O. mykiss (p < 0.05). Dilution of salmonids semen in a sodium alginate‐based extender is effective for protecting sperm quality during 10 days of short‐term storage.

[1]  A. Agarwal,et al.  Novel insights into the pathophysiology of varicocele and its association with reactive oxygen species and sperm DNA fragmentation , 2015, Asian journal of andrology.

[2]  J. Alvarez,et al.  Supplementation of IVF medium with melatonin: effect on sperm functionality and in vitro produced bovine embryos , 2015, Andrologia.

[3]  E. Figueroa,et al.  Effect of short‐term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry , 2015, Andrologia.

[4]  E. Figueroa,et al.  Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification. , 2015, Theriogenology.

[5]  G. Dietrich,et al.  Effect of postthaw storage time and sperm-to-egg ratio on fertility of cryopreserved brook trout sperm. , 2015, Theriogenology.

[6]  A. Agarwal,et al.  Utility of antioxidants during assisted reproductive techniques: an evidence based review , 2014, Reproductive Biology and Endocrinology.

[7]  Xiuzhu Sun,et al.  Effects of alginate on frozen-thawed boar spermatozoa quality, lipid peroxidation and antioxidant enzymes activities. , 2014, Animal reproduction science.

[8]  M. Aguilar-Júarez,et al.  Cold storage of the sperm of the endemic trout Oncorhynchus mykiss nelsoni: a strategy for short-term germplasm conservation of endemic species , 2014 .

[9]  M. Kalbassi,et al.  Effects of short-term storage on the motility, oxidative stress, and ATP content of Persian sturgeon (Acipenser persicus) sperm. , 2013, Animal reproduction science.

[10]  M. Hulák,et al.  Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm. , 2013, Animal reproduction science.

[11]  E. Figueroa,et al.  Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters , 2013 .

[12]  M. Pšenička,et al.  Sperm biology and control of reproduction in sturgeon: (II) sperm morphology, acrosome reaction, motility and cryopreservation , 2012, Reviews in Fish Biology and Fisheries.

[13]  N. Mansour,et al.  The effect of antioxidants on the quality of cryopreserved semen in two salmonid fish, the brook trout (Salvelinus fontinalis) and the rainbow trout (Oncorhynchus mykiss). , 2011, Theriogenology.

[14]  M. T. Dinis,et al.  The influence of certain aminoacids and vitamins on post-thaw fish sperm motility, viability and DNA fragmentation. , 2011, Animal reproduction science.

[15]  E. Figueroa,et al.  Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: stability of mitochondrion as criterion of effectiveness. , 2011, Animal reproduction science.

[16]  M. Hulák,et al.  Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques1 , 2010, Biology of reproduction.

[17]  N. Mansour,et al.  A comparative study on antioxidant systems in semen of species of the Percidae, Salmonidae, Cyprinidae, and Lotidae for improving semen storage techniques , 2010 .

[18]  S. Nimrat,et al.  Chilled storage of walking catfish (Clarias macrocephalus) semen , 2009 .

[19]  P. de Paz,et al.  Evaluation of oxidative DNA damage promoted by storage in sperm from sex-reversed rainbow trout. , 2009, Theriogenology.

[20]  Huashi Guan,et al.  In vitro antioxidative activities of three marine oligosaccharides , 2007, Natural product research.

[21]  R. Aitken,et al.  Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa. , 2006, The Journal of clinical endocrinology and metabolism.

[22]  E. Mascha,et al.  Human sperm superoxide anion generation and correlation with semen quality in patients with male infertility. , 2004, Fertility and sterility.

[23]  A. Agarwal,et al.  Role of reactive oxygen species in the pathophysiology of human reproduction. , 2003, Fertility and sterility.

[24]  R. Patzner,et al.  Aging Processes of Rainbow Trout Semen during Storage , 1997 .