Advanced Near‐Infrared Light for Monitoring and Modulating the Spatiotemporal Dynamics of Cell Functions in Living Systems

Abstract Light‐based technique, including optical imaging and photoregulation, has become one of the most important tools for both fundamental research and clinical practice, such as cell signal sensing, cancer diagnosis, tissue engineering, drug delivery, visual regulation, neuromodulation, and disease treatment. In particular, low energy near‐infrared (NIR, 700–1700 nm) light possesses lower phototoxicity and higher tissue penetration depth in living systems as compared with ultraviolet/visible light, making it a promising tool for in vivo applications. Currently, the NIR light‐based imaging and photoregulation strategies have offered a possibility to real‐time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy. Herein, the recent progress with respect to NIR light for monitoring and modulating the spatiotemporal dynamics of cell functions in living systems are summarized. In particular, the applications of NIR light‐based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed. In addition, the challenges and prospects for NIR light‐based cell sensing and regulating techniques are comprehensively discussed.

[1]  Qiangbin Wang,et al.  Challenges and Opportunities for Intravital Near-Infrared Fluorescence Imaging Technology in the Second Transparency Window. , 2018, ACS nano.

[2]  Igor L. Medintz,et al.  Evaluating the potential of using quantum dots for monitoring electrical signals in neurons , 2018, Nature Nanotechnology.

[3]  Charles P. Lin,et al.  An authentic imaging probe to track cell fate from beginning to end , 2014, Nature Communications.

[4]  Viviana Gradinaru,et al.  Neuroscience: Fluorescent boost for voltage sensors , 2016, Nature.

[5]  Hartmut Jaeschke,et al.  Apoptosis and necrosis in liver disease , 2004, Liver international : official journal of the International Association for the Study of the Liver.

[6]  Fang Liu,et al.  Near-infrared light-mediated photoactivation of a platinum antitumor prodrug and simultaneous cellular apoptosis imaging by upconversion-luminescent nanoparticles. , 2014, Angewandte Chemie.

[7]  R. Weissleder,et al.  Near-infrared fluorescent imaging of tumor apoptosis. , 2003, Cancer research.

[8]  Polina Anikeeva,et al.  Remotely Controlled Chemomagnetic Modulation of Targeted Neural Circuits , 2019, Nature Nanotechnology.

[9]  Jin-Moo Lee,et al.  Photo-activatable Cre recombinase regulates gene expression in vivo , 2015, Scientific Reports.

[10]  Quli Fan,et al.  A Photoacoustic Probe for the Imaging of Tumor Apoptosis by Caspase-Mediated Macrocyclization and Self-Assembly. , 2019, Angewandte Chemie.

[11]  Wenpei Fan,et al.  A Rationally Designed Semiconducting Polymer Brush for NIR‐II Imaging‐Guided Light‐Triggered Remote Control of CRISPR/Cas9 Genome Editing , 2019, Advanced materials.

[12]  Yunxia Li,et al.  Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears , 2019, Advanced science.

[13]  Yun Huang,et al.  Near-infrared photoactivatable control of Ca2+ signaling and optogenetic immunomodulation , 2015, eLife.

[14]  Heemin Kang,et al.  Remote Control of Intracellular Calcium Using Upconversion Nanotransducers Regulates Stem Cell Differentiation In Vivo , 2018, Advanced Functional Materials.

[15]  Chenjie Xu,et al.  Nanosensors for Continuous and Noninvasive Monitoring of Mesenchymal Stem Cell Osteogenic Differentiation. , 2016, Small.

[16]  Fan Yang,et al.  Time-resolved protein activation by proximal decaging in living systems , 2019, Nature.

[17]  Kai Cai,et al.  Au Hollow Nanorods-Chimeric Peptide Nanocarrier for NIR-II Photothermal Therapy and Real-time Apoptosis Imaging for Tumor Theranostics , 2019, Theranostics.

[18]  Manu M. Joseph,et al.  A Ratiometric Near-Infrared Fluorogen for the Real Time Visualization of Intracellular Redox Status during Apoptosis. , 2017, Chemistry.

[19]  Evan W. Miller,et al.  Small molecule fluorescent voltage indicators for studying membrane potential. , 2016, Current opinion in chemical biology.

[20]  Qiangbin Wang,et al.  Advanced NIR‐II Fluorescence Imaging Technology for In Vivo Precision Tumor Theranostics , 2019, Advanced Therapeutics.

[21]  Michael R Hamblin,et al.  Far infrared radiation (FIR): Its biological effects and medical applications , 2012, Photonics & lasers in medicine.

[22]  Yuanhuan Yu,et al.  Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation , 2018, Proceedings of the National Academy of Sciences.

[23]  Rui Ji,et al.  An NIR‐II Fluorescence/Dual Bioluminescence Multiplexed Imaging for In Vivo Visualizing the Location, Survival, and Differentiation of Transplanted Stem Cells , 2018, Advanced Functional Materials.

[24]  Shuo Shi,et al.  Tumor‐Targeted Drug and CpG Delivery System for Phototherapy and Docetaxel‐Enhanced Immunotherapy with Polarization toward M1‐Type Macrophages on Triple Negative Breast Cancers , 2019, Advanced materials.

[25]  Zhe Wang,et al.  Bioinspired Nanocomplex for Spatiotemporal Imaging of Sequential mRNA Expression in Differentiating Neural Stem Cells , 2014, ACS nano.

[26]  Peng Zou,et al.  Hybrid Indicators for Fast and Sensitive Voltage Imaging. , 2018, Angewandte Chemie.

[27]  Ling Huang,et al.  Illuminating Cell Signaling with Near-Infrared Light-Responsive Nanomaterials. , 2016, ACS nano.

[28]  Lukasz Kowalik,et al.  Illuminating developmental biology through photochemistry. , 2017, Nature chemical biology.

[29]  Seok Hyun Yun,et al.  Upconversion Nanoparticles/Hyaluronate-Rose Bengal Conjugate Complex for Noninvasive Photochemical Tissue Bonding. , 2017, ACS nano.

[30]  Dan Ding,et al.  Regulating Near-Infrared Photodynamic Properties of Semiconducting Polymer Nanotheranostics for Optimized Cancer Therapy. , 2017, ACS nano.

[31]  Shengliang Li,et al.  Photothermal‐Responsive Conjugated Polymer Nanoparticles for Remote Control of Gene Expression in Living Cells , 2018, Advanced materials.

[32]  Li Guo,et al.  Real-time imaging of single nerve cell apoptosis in retinal neurodegeneration. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[33]  Jeremy Freeman,et al.  Technologies for imaging neural activity in large volumes , 2016, Nature Neuroscience.

[34]  Allan R. Jones,et al.  A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing , 2012, Nature Neuroscience.

[35]  Aimin Yu,et al.  Gold‐Nanorod‐Assisted Near‐Infrared Stimulation of Primary Auditory Neurons , 2014, Advanced healthcare materials.

[36]  Fan Zhang,et al.  In Vivo High-resolution Ratiometric Fluorescence Imaging of Inflammation Using NIR-II Nanoprobes with 1550 nm Emission. , 2019, Nano letters.

[37]  Yufeng Zheng,et al.  Photoelectric-Responsive Extracellular Matrix for Bone Engineering. , 2019, ACS nano.

[38]  Michael Z. Lin,et al.  Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome , 2009, Science.

[39]  Bozhi Tian,et al.  Photoelectrochemical modulation of neuronal activity with free-standing coaxial silicon nanowires , 2018, Nature Nanotechnology.

[40]  Chin-Tu Chen,et al.  Rational design of silicon structures for optically controlled multiscale biointerfaces , 2018, Nature Biomedical Engineering.

[41]  G. Daley,et al.  The promise of induced pluripotent stem cells in research and therapy , 2012, Nature.

[42]  Jiye Shi,et al.  Hydrogen Sulfide-Activatable Second Near-Infrared Fluorescent Nanoassemblies for Targeted Photothermal Cancer Therapy. , 2018, Nano letters.

[43]  D. J. Harrison,et al.  Bright and fast multi-colored voltage reporters via electrochromic FRET , 2014, Nature Communications.

[44]  Yan Zhang,et al.  In vivo real-time visualization of tissue blood flow and angiogenesis using Ag2S quantum dots in the NIR-II window. , 2014, Biomaterials.

[45]  Federico Díaz-González,et al.  Up-regulation of gelatinases in the colorectal adenoma-carcinoma sequence. , 2006, European journal of cancer.

[46]  J. Irish,et al.  Role of glutathione depletion and reactive oxygen species generation in apoptotic signaling in a human B lymphoma cell line , 2002, Cell Death and Differentiation.

[47]  M. Iruela-Arispe,et al.  Membrane lipids and cell signaling , 2017, Current opinion in lipidology.

[48]  Kenji F. Tanaka,et al.  Near-infrared deep brain stimulation via upconversion nanoparticle–mediated optogenetics , 2018, Science.

[49]  Bozhi Tian,et al.  Heterogeneous silicon mesostructures for lipid-supported bioelectric interfaces , 2016, Nature materials.

[50]  Jelena Platisa,et al.  Genetically encoded fluorescent voltage indicators: are we there yet? , 2018, Current Opinion in Neurobiology.

[51]  D. Kleinfeld,et al.  ReaChR: A red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation , 2013, Nature Neuroscience.

[52]  Zheng Ruan,et al.  Polypeptide-Conjugated Second Near-Infrared Organic Fluorophore for Image-Guided Photothermal Therapy. , 2019, ACS nano.

[53]  Byeong-Cheol Ahn,et al.  In Vivo Cell Tracking with Bioluminescence Imaging , 2015, Nuclear Medicine and Molecular Imaging.

[54]  Yan Lyu,et al.  Semiconducting Polymer Nanobioconjugates for Targeted Photothermal Activation of Neurons. , 2016, Journal of the American Chemical Society.

[55]  Mohamady El-Gaby,et al.  Archaerhodopsin Selectively and Reversibly Silences Synaptic Transmission through Altered pH , 2016, Cell reports.

[56]  S. Elmore.  Apoptosis: A Review of Programmed Cell Death , 2007, Toxicologic pathology.

[57]  Hongjie Dai,et al.  Near-infrared fluorophores for biomedical imaging , 2017, Nature Biomedical Engineering.

[58]  M. C. Mancini,et al.  Bioimaging: second window for in vivo imaging. , 2009, Nature nanotechnology.

[59]  Jingchao Li,et al.  Nanotransducers for Near‐Infrared Photoregulation in Biomedicine , 2019, Advanced materials.

[60]  Quli Fan,et al.  Recent Advances on Activatable NIR‐II Fluorescence Probes for Biomedical Imaging , 2019, Advanced Optical Materials.

[61]  Peng Li,et al.  Redox-Responsive Fluorescent Probes with Different Design Strategies. , 2015, Accounts of chemical research.

[62]  Guangcun Chen,et al.  An Activatable NIR-II Nanoprobe for In Vivo Early Real-Time Diagnosis of Traumatic Brain Injury. , 2019, Angewandte Chemie.

[63]  Eric C Greenwald,et al.  Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks. , 2018, Chemical reviews.

[64]  Francisco Bezanilla,et al.  Real-time imaging of electrical signals with an infrared FDA-approved dye. , 2014, Biophysical journal.

[65]  Xu Cao,et al.  The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine , 2013, Nature Medicine.

[66]  Ralph Weissleder,et al.  Near-infrared fluorescence: application to in vivo molecular imaging. , 2010, Current opinion in chemical biology.

[67]  Yebin Jung,et al.  Cancer-Microenvironment-Sensitive Activatable Quantum Dot Probe in the Second Near-Infrared Window. , 2017, Nano letters.

[68]  Z. Dai,et al.  Photothermal therapy and photoacoustic imaging via nanotheranostics in fighting cancer. , 2019, Chemical Society reviews.

[69]  Valentina Marchesano,et al.  Control of Wnt/β-Catenin Signaling Pathway in Vivo via Light Responsive Capsules. , 2016, ACS nano.

[70]  Michael Z. Lin,et al.  The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins. , 2017, Trends in biochemical sciences.

[71]  Yijun Yang,et al.  In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles , 2019, Nature Biotechnology.

[72]  Leonardo Sacconi,et al.  Palette of fluorinated voltage-sensitive hemicyanine dyes , 2012, Proceedings of the National Academy of Sciences.

[73]  Yan Zhang,et al.  In vivo real-time visualization of mesenchymal stem cells tropism for cutaneous regeneration using NIR-II fluorescence imaging. , 2015, Biomaterials.

[74]  Michael Kahn,et al.  Wnt/Catenin Signaling in Adult Stem Cell Physiology and Disease , 2014, Stem Cell Reviews and Reports.

[75]  Irma Martišienė,et al.  Voltage-Sensitive Fluorescence of Indocyanine Green in the Heart. , 2016, Biophysical journal.

[76]  Shuo Diao,et al.  A small-molecule dye for NIR-II imaging. , 2016, Nature materials.

[77]  Z. Werb,et al.  New functions for the matrix metalloproteinases in cancer progression , 2002, Nature Reviews Cancer.

[78]  W. Wilson,et al.  Targeting hypoxia in cancer therapy , 2011, Nature Reviews Cancer.

[79]  Stephanie C. Seeman,et al.  Bright and photostable chemigenetic indicators for extended in vivo voltage imaging , 2018, Science.

[80]  Qiangbin Wang,et al.  Real-Time Monitoring Surface Chemistry-Dependent In Vivo Behaviors of Protein Nanocages via Encapsulating an NIR-II Ag2S Quantum Dot. , 2015, ACS nano.

[81]  Selmaan N. Chettih,et al.  Voltage imaging and optogenetics reveal behavior dependent changes in hippocampal dynamics , 2019, Nature.

[82]  Nina Vogt,et al.  Voltage sensors: challenging, but with potential , 2015, Nature Methods.

[83]  Yuliang Zhao,et al.  Near-infrared Light-Initiated Hybridization Chain Reaction for Spatially- and Temporally-Resolved Signal Amplification. , 2019, Angewandte Chemie.

[84]  C. Bortner,et al.  The role of DNA fragmentation in apoptosis. , 1995, Trends in cell biology.

[85]  David Piwnica-Worms,et al.  An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging. , 2009, Bioconjugate chemistry.

[86]  Feng Zhang,et al.  Multimodal fast optical interrogation of neural circuitry , 2007, Nature.

[87]  C. DeForest,et al.  Photoresponsive biomaterials for targeted drug delivery and 4D cell culture , 2018 .

[88]  Fan Zhang,et al.  Peroxynitrite Activatable NIR-II Fluorescent Molecular Probe for Drug-Induced Hepatotoxicity Monitoring. , 2019, Analytical chemistry.

[89]  L. Ngo,et al.  The FLARE™ Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping , 2009, Annals of Surgical Oncology.

[90]  Guangcun Chen,et al.  Revealing the Fate of Transplanted Stem Cells In Vivo with a Novel Optical Imaging Strategy. , 2018, Small.

[91]  Mahdi Karimi,et al.  Smart Nanostructures for Cargo Delivery: Uncaging and Activating by Light. , 2017, Journal of the American Chemical Society.

[92]  Matthias Lamping,et al.  Light-Driven Chloride Transport Kinetics of Halorhodopsin. , 2018, Biophysical journal.

[93]  Igor L. Medintz,et al.  Quantum Dot-Peptide-Fullerene Bioconjugates for Visualization of in Vitro and in Vivo Cellular Membrane Potential. , 2017, ACS nano.

[94]  Andreas Möglich,et al.  Blue-Light Receptors for Optogenetics. , 2018, Chemical reviews.

[95]  Shasha He,et al.  Photolabile Semiconducting Polymer Nanotransducer for Near-infrared Regulation of CRISPR/Cas9 Gene Editing. , 2019, Angewandte Chemie.

[96]  Jianghong Rao,et al.  Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing , 2014, Nature Biotechnology.

[97]  T. Knöpfel,et al.  Genetically encoded voltage indicators for large scale cortical imaging come of age. , 2015, Current opinion in chemical biology.

[98]  Walther Akemann,et al.  Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements , 2008, PloS one.

[99]  Satoshi Arai,et al.  Gold Nanoshell-Mediated Remote Myotube Activation. , 2017, ACS nano.

[100]  Hong-Yuan Chen,et al.  Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly. , 2019, Journal of the American Chemical Society.

[101]  Jin Bao,et al.  Mammalian Near-Infrared Image Vision through Injectable and Self-Powered Retinal Nanoantennae , 2019, Cell.

[102]  Yongchun Pan,et al.  Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform , 2019, Science Advances.

[103]  N. N. Petrishchev,et al.  Medical applications of mid-IR lasers. Problems and prospects , 2010 .

[104]  Hui-wang Ai,et al.  Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors. , 2019, Annual review of analytical chemistry.

[105]  Yan Zhang,et al.  An Upconversion Nanoparticle Enables Near Infrared-Optogenetic Manipulation of the Caenorhabditis elegans Motor Circuit. , 2019, ACS nano.

[106]  Yunsook Choi,et al.  Historical Overview and General Methods of Membrane Potential Imaging. , 2015, Advances in experimental medicine and biology.

[107]  Xianmin Zeng,et al.  A platform for rapid generation of single and multiplexed reporters in human iPSC lines , 2015, Scientific Reports.

[108]  Daria M. Shcherbakova,et al.  Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging , 2016, Nature Communications.

[109]  Wei Fan,et al.  Dye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications. , 2016, ACS nano.

[110]  B M Salzberg,et al.  A large change in axon fluorescence that provides a promising method for measuring membrane potential. , 1973, Nature: New biology.

[111]  Guangcun Chen,et al.  Recent Advances in Tracking the Transplanted Stem Cells Using Near‐Infrared Fluorescent Nanoprobes: Turning from the First to the Second Near‐Infrared Window , 2018, Advanced healthcare materials.

[112]  Shuo Diao,et al.  Ultrafast fluorescence imaging in vivo with conjugated polymer fluorophores in the second near-infrared window , 2014, Nature Communications.

[113]  Junle Qu,et al.  Crucial breakthrough of second near-infrared biological window fluorophores: design and synthesis toward multimodal imaging and theranostics. , 2018, Chemical Society reviews.

[114]  Steven Ripp,et al.  The Expanding Toolbox of In Vivo Bioluminescent Imaging , 2016, Front. Oncol..

[115]  Shaoqin Gong,et al.  NIR‐induced spatiotemporally controlled gene silencing by upconversion nanoparticle‐based siRNA nanocarrier , 2017, Journal of controlled release : official journal of the Controlled Release Society.

[116]  Bin Liu,et al.  Recent Advances of Optical Imaging in the Second Near‐Infrared Window , 2018, Advanced materials.

[117]  Cara Chlebicki,et al.  Novel wavelengths for laser nerve stimulation , 2010, Lasers in surgery and medicine.

[118]  Rui Tian,et al.  Near‐Infrared‐II Molecular Dyes for Cancer Imaging and Surgery , 2019, Advanced materials.

[119]  Claus-Peter Richter,et al.  Infrared neural stimulation in the cochlea , 2013, Photonics West - Biomedical Optics.

[120]  Guangcun Chen,et al.  Glutathione-capped quantum dots for plasma membrane labeling and membrane potential imaging , 2019, Nano Research.

[121]  Andreas U Bayer,et al.  Association of glaucoma with neurodegenerative diseases with apoptotic cell death: Alzheimer's disease and Parkinson's disease. , 2002, American journal of ophthalmology.

[122]  Hao Li,et al.  Near-Infrared Light-Activated DNA-Agonist Nanodevice for Nongenetically and Remotely Controlled Cellular Signaling and Behaviors in Live Animals. , 2019, Nano letters.

[123]  Hideyuki Okano,et al.  Single-cell bioluminescence imaging of deep tissue in freely moving animals , 2018, Science.

[124]  Xiaoqun Gong,et al.  Near-Infrared Light Triggered Upconversion Optogenetic Nanosystem for Cancer Therapy. , 2017, ACS nano.

[125]  E. Bamberg,et al.  Channelrhodopsin-2, a directly light-gated cation-selective membrane channel , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[126]  Vladislav V Verkhusha,et al.  Near-Infrared Fluorescent Proteins, Biosensors, and Optogenetic Tools Engineered from Phytochromes. , 2017, Chemical reviews.

[127]  F. Bezanilla,et al.  Photosensitivity of Neurons Enabled by Cell-Targeted Gold Nanoparticles , 2015, Neuron.

[128]  J. Pouysségur,et al.  Hypoxia signalling in cancer and approaches to enforce tumour regression , 2006, Nature.

[129]  Demosthenes P. Morales,et al.  Light-Triggered Genome Editing: Cre Recombinase Mediated Gene Editing with Near-Infrared Light. , 2018, Small.

[130]  Evan W. Miller,et al.  Spying on Neuronal Membrane Potential with Genetically Targetable Voltage Indicators. , 2018, Journal of the American Chemical Society.

[131]  Mark Gomelsky,et al.  Engineering Adenylate Cyclase Activated by Near-Infrared Window Light for Mammalian Optogenetic Applications. , 2019, ACS synthetic biology.

[132]  Christina K. Kim,et al.  Molecular tools for imaging and recording neuronal activity , 2019, Nature Chemical Biology.

[133]  Bernard Hoflack,et al.  Bridging membrane and cytoskeleton dynamics in the secretory and endocytic pathways , 2011, Nature Cell Biology.

[134]  Zhigang Chen,et al.  Near‐Infrared‐Light Activatable Nanoparticles for Deep‐Tissue‐Penetrating Wireless Optogenetics , 2019, Advanced healthcare materials.

[135]  Weijian Yang,et al.  In vivo imaging of neural activity , 2017, Nature Methods.

[136]  Jingchao Li,et al.  Development of organic semiconducting materials for deep-tissue optical imaging, phototherapy and photoactivation. , 2019, Chemical Society reviews.

[137]  N. Harbeck,et al.  Molecular and protein markers for clinical decision making in breast cancer: today and tomorrow. , 2014, Cancer treatment reviews.

[138]  Yu Ji,et al.  “Dual Lock‐and‐Key”‐Controlled Nanoprobes for Ultrahigh Specific Fluorescence Imaging in the Second Near‐Infrared Window , 2018, Advanced materials.

[139]  Bong Hoon Kim,et al.  A Wireless Closed Loop System for Optogenetic Peripheral Neuromodulation , 2018, Nature.

[140]  Ben Zhong Tang,et al.  Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF4:Yb3+Tm3+@SiO2 Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation. , 2017, ACS applied materials & interfaces.

[141]  Jingchao Li,et al.  Near‐Infrared Photoactivatable Semiconducting Polymer Nanoblockaders for Metastasis‐Inhibited Combination Cancer Therapy , 2019, Advanced materials.

[142]  Nitish Thakor,et al.  Expanding the Toolbox of Upconversion Nanoparticles for In Vivo Optogenetics and Neuromodulation , 2019, Advanced materials.

[143]  M. Mimeault,et al.  Stem Cells: A Revolution in Therapeutics—Recent Advances in Stem Cell Biology and Their Therapeutic Applications in Regenerative Medicine and Cancer Therapies , 2007, Clinical pharmacology and therapeutics.

[144]  Luke D. Lavis,et al.  Isomeric tuning yields bright and targetable red Ca2+ indicators. , 2019, Journal of the American Chemical Society.

[145]  J. Spudich,et al.  Ultrasensitive Measurements of Microbial Rhodopsin Photocycles Using Photochromic FRET , 2012, Photochemistry and photobiology.

[146]  Feng Xing,et al.  Novel concept of the smart NIR-light–controlled drug release of black phosphorus nanostructure for cancer therapy , 2018, Proceedings of the National Academy of Sciences.

[147]  Brant C. Gibson,et al.  Near‐Infrared Fluorescent Nanomaterials for Bioimaging and Sensing , 2017 .

[148]  Polina Anikeeva,et al.  Wireless magnetothermal deep brain stimulation , 2015, Science.

[149]  Suhasa B Kodandaramaiah,et al.  Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes in Neuroimaging. , 2017, Biophysical journal.

[150]  Ping Gong,et al.  Hypoxia-triggered single molecule probe for high-contrast NIR II/PA tumor imaging and robust photothermal therapy , 2018, Theranostics.

[151]  Fan Zhang,et al.  Stable, Wavelength-Tunable Fluorescent Dyes in the NIR-II Region for In Vivo High-Contrast Bioimaging and Multiplexed Biosensing. , 2019, Angewandte Chemie.

[152]  John-Christopher Boyer,et al.  Near infrared light triggered release of biomacromolecules from hydrogels loaded with upconversion nanoparticles. , 2012, Journal of the American Chemical Society.