Structure of benzyl T-antigen disaccharide bound to Amaranthus caudatus agglutinin

Amaranthus caudatus agglutinin contains a novel arrangement of four beta-trefoil domains. The sugar-binding site provides specificity for the carcinoma-associated T-antigen disaccharide even when 'masked' by other sugars.

[1]  C. Wright.  Crystal structure of a wheat germ agglutinin/glycophorin-sialoglycopeptide receptor complex. Structural basis for cooperative lectin-cell binding. , 1992, The Journal of biological chemistry.

[2]  S M Gruner,et al.  Charge coupled device X-ray detectors for macromolecular crystallography. , 1995, Structure.

[3]  R M Sweet,et al.  Crystal structure of the complex of porcine trypsin with soybean trypsin inhibitor (Kunitz) at 2.6-A resolution. , 1974, Biochemistry.

[4]  S. Barondes,et al.  X-ray crystal structure of the human dimeric S-Lac lectin, L-14-II, in complex with lactose at 2.9-A resolution. , 1994, The Journal of biological chemistry.

[5]  G J Williams,et al.  The Protein Data Bank: a computer-based archival file for macromolecular structures. , 1977, Journal of molecular biology.

[6]  T. Holak,et al.  Structure of hisactophilin is similar to interleukin-1β and fibroblast growth factor , 1994, Nature.

[7]  J. Zou,et al.  Improved methods for building protein models in electron density maps and the location of errors in these models. , 1991, Acta crystallographica. Section A, Foundations of crystallography.

[8]  R. Hamlin,et al.  [27] Multiwire area X-ray diffractometers , 1985 .

[9]  D. Rees,et al.  Three-dimensional structures of acidic and basic fibroblast growth factors. , 1993, Science.

[10]  Vivek Sharma,et al.  A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a β-prism fold , 1996, Nature Structural Biology.

[11]  P. Kraulis.  A program to produce both detailed and schematic plots of protein structures , 1991 .

[12]  A T Brünger,et al.  Slow-cooling protocols for crystallographic refinement by simulated annealing. , 1990, Acta crystallographica. Section A, Foundations of crystallography.

[13]  B. Hazes,et al.  The (QxW)3 domain: A flexible lectin scaffold , 1996, Protein science : a publication of the Protein Society.

[14]  N. Xuong,et al.  Software for a diffractometer with multiwire area detector. , 1985, Methods in enzymology.

[15]  J. Robertus,et al.  Structure of ricin B‐chain at 2.5 Å resolution , 1991, Proteins.

[16]  I. Goldstein,et al.  Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. , 1989, The Journal of biological chemistry.

[17]  Alexey G. Murzin,et al.  β-Trefoil fold: Patterns of structure and sequence in the Kunitz inhibitors interleukins-1β and 1α and fibroblast growth factors , 1992 .

[18]  Barry C. Finzel,et al.  Crystal structure of recombinant human interleukin-1β at 2·0 Å resolution , 1989 .

[19]  A. Mclachlan.  Three-fold structural pattern in the soybean trypsin inhibitor (Kunitz). , 1979, Journal of molecular biology.

[20]  N. Vietmeyer.  Lesser-Known Plants of Potential Use in Agriculture and Forestry , 1986, Science.

[21]  A. Brunger.  Free R value: a novel statistical quantity for assessing the accuracy of crystal structures. , 1992 .

[22]  N. Sharon,et al.  Structure of a legume lectin with an ordered N-linked carbohydrate in complex with lactose. , 1991, Science.

[23]  G. Waksman,et al.  FGF binding and FGF receptor activation by synthetic heparan-derived di- and trisaccharides. , 1995, Science.

[24]  L. Roberts,et al.  Ricin: structure, mode of action, and some current applications , 1994, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[25]  R M Esnouf,et al.  An extensively modified version of MolScript that includes greatly enhanced coloring capabilities. , 1997, Journal of molecular graphics & modelling.

[26]  W. Hol,et al.  Tumor marker disaccharide D‐Gal‐β1,3‐GalNAc complexed to heat‐labile enterotoxin from Escherichia coli , 1996, Protein science : a publication of the Protein Society.

[27]  I. Goldstein,et al.  Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds. , 1990, Biochemistry.

[28]  A. Raina,et al.  Molecular cloning of a gene encoding a seed-specific protein with nutritionally balanced amino acid composition from Amaranthus. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[29]  S. Cusack,et al.  Structure of the influenza virus haemagglutinin complexed with its receptor, sialic acid , 1988, Nature.

[30]  C. Boland,et al.  Use of the lectin from Amaranthus caudatus as a histochemical probe of proliferating colonic epithelial cells. , 1991, Cancer Research.

[31]  D Eisenberg,et al.  3D domain swapping: A mechanism for oligomer assembly , 1995, Protein science : a publication of the Protein Society.

[32]  E A Merritt,et al.  Raster3D Version 2.0. A program for photorealistic molecular graphics. , 1994, Acta crystallographica. Section D, Biological crystallography.

[33]  D C Rees,et al.  Heparin Structure and Interactions with Basic Fibroblast Growth Factor , 1996, Science.

[34]  I. Goldstein,et al.  Studies on the Thomsen-Friedenreich antigen in human colon with the lectin Amaranthin. Normal and neoplastic epithelium express only cryptic T antigen. , 1992, Laboratory investigation; a journal of technical methods and pathology.

[35]  Alexander McPherson,et al.  Preparation and analysis of protein crystals , 1982 .

[36]  B. C. Wang.  Resolution of phase ambiguity in macromolecular crystallography. , 1985, Methods in enzymology.

[37]  T. Sixma,et al.  Lactose binding to heat-labile enterotoxin revealed by X-ray crystallography , 1992, Nature.

[38]  M Vijayan,et al.  Conformation, protein-carbohydrate interactions and a novel subunit association in the refined structure of peanut lectin-lactose complex. , 1996, Journal of molecular biology.

[39]  Kuo-Sen Huang,et al.  Insight into E-selectin/ligand interaction from the crystal structure and mutagenesis of the lec/EGF domains , 1994, Nature.

[40]  F. Quiocho.  Molecular features and basic understanding of protein-carbohydrate interactions: the arabinose-binding protein-sugar complex. , 1988, Current topics in microbiology and immunology.