Study of Regulation of Long-Chain Fatty Acid Metabolism Using Computer Analysis of Complete Bacterial Genomes

One of the main trends in the prokaryote genomics is the comparative analysis of metabolic pathways. This method can be used for the analysis of experimentally studied systems of co-regulated genes, as well as genes with unknown regulatory signals. In this study we apply the comparative analysis of regulatory signals to the genes of the enzymes for fatty acid metabolism from Escherichia coli, Haemophilus influenzae, Vibrio cholerae, and Yersinia pestis. Transcription of these genes is regulated by the FadR protein. We describe the FadR regulation of long-chain fatty acid oxidation and partial regulation of fatty acid biosynthesis. We also demonstrate that the gene yafHencoding acyl-CoA dehydrogenase is identical to the gene fadEpreviously identified by genetic techniques.

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