An experimental infection to investigate the indirect transmission of classical swine fever virus by excretions of infected pigs.

In this experiment transmission of classical swine fever (CSF) virus via excretions of infected pigs was investigated under experimental conditions. Five pairs of pigs were experimentally infected with CSF virus. Eight days after experimental infection, when all pigs were viraemic for at least 3 days, the pens were depopulated and 20 h later, restocked with five pairs of susceptible pigs which stayed in these pens for 35 days. During the first 3 weeks of the experiment, the pens were neither cleaned nor disinfected. During the observation period, none of the susceptible pigs became infected. This result indicates that CSF virus spread via excretions is of minor importance in the early stages of infection. For extrapolation of these findings to the field situation and to increase the validity of the conclusions further research is needed to evaluate the effect of factors like virus strain, interval, ..., that may influence the outcome of the experiment.

[1]  M C M de Jong,et al.  Rate of inter-herd transmission of classical swine fever virus by different types of contact during the 1997–8 epidemic in The Netherlands , 2002, Epidemiology and Infection.

[2]  A. de Kruif,et al.  An experimental infection with classical swine fever virus in pregnant sows: transmission of the virus, course of the disease, antibody response and effect on gestation. , 2008, Journal of veterinary medicine. B, Infectious diseases and veterinary public health.

[3]  J. Stegeman,et al.  Factors associated with the introduction of classical swine fever virus into pig herds in the central area of the 1997/98 epidemic in the Netherlands , 2001, Veterinary Record.

[4]  A. de Kruif,et al.  Airborne transmission of classical swine fever virus under experimental conditions , 2000, Veterinary Record.

[5]  A de Kruif,et al.  An experimental infection with classical swine fever in E2 sub-unit marker-vaccine vaccinated and in non-vaccinated pigs. , 2000, Vaccine.

[6]  S. Edwards Survival and inactivation of classical swine fever virus. , 2000, Veterinary microbiology.

[7]  A. Kruif,et al.  Estimation of the likelihood for Neighbourhood infections during classical swine fever epidemics based on a spatial risk assessment of real outbreak data , 2000 .

[8]  A. de Kruif,et al.  Experimental infection of slaughter pigs with classical swine fever virus: transmission of the virus, course of the disease and antibody response , 1999, Veterinary Record.

[9]  A. Bouma,et al.  Transmission of classical swine fever virus by artificial insemination. , 1999, Veterinary microbiology.

[10]  A. McGoldrick,et al.  Closed one-tube reverse transcription nested polymerase chain reaction for the detection of pestiviral RNA with fluorescent probes. , 1999, Journal of virological methods.

[11]  J. Oirschot Classical swine fever , 1999 .

[12]  A de Kruif,et al.  An experimental infection with classical swine fever virus in weaner pigs. I. Transmission of the virus, course of the disease, and antibody response. , 1998, The Veterinary quarterly.

[13]  H. S. Horst,et al.  Risks and economic consequences of introducing classical swine fever into The Netherlands by feeding swill to swine. , 1997, Revue scientifique et technique.

[14]  C. Terpstra,et al.  Epizootiology of Hog Cholera , 1988 .

[15]  Jensen Mh Detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. A comparative examination using CF, PLA and NPLA assays. , 1981 .

[16]  J. V. van Bekkum,et al.  Virus excretion in vaccinated pigs subject to contact infection with virulent hog cholera strains. , 2010, Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B.

[17]  R. W. Hughes,et al.  Some factors that may influence hog cholera transmission. , 1960, American journal of veterinary research.