Microinjection molded disposable microfluidic lab-on-a-chip for efficient detection of agglutination

Previous diagnosing methods based on agglutination have a limitation in view of emergency and point-of-care diagnoses due to the requirement of large scale equipments and much agglutination time. In this paper, we propose a low cost microfluidic lab-on-a-chip for more efficient detection of agglutination. In the present lab-on-a-chip, two inlet microwells, flow guiding microchannels, chaotic micromixer and reaction microwell are fully integrated. Mold inserts for the lab-on-a-chip were manufactured by UV photolithography and nickel electroplating process. The complete lab-on-a-chip was realized by the microinjection molding of cyclic olefin copolymer and the subsequent thermal bonding. The improved serpentine laminating micromixer, developed by our group, integrated in the lab-on-a-chip showed the high-level of chaotic mixing, thereby enabling us to get a reliable mixing of sample and reagent. The performance of the fabricated lab-on-a-chip was demonstrated by agglutination experiments with simulated bloods of 10 μl and simulated sera of 10 μl. The results of agglutination inside the reaction microwell were clearly read by means of the level of light transmission. The present microfluidic lab-on-a-chip could be widely applied to various clinical diagnostics based on agglutination tests.

[1]  Dong Sung Kim,et al.  Collapse-free thermal bonding technique for large area microchambers in plastic lab-on-a-chip applications , 2007 .

[2]  Darwin R. Reyes,et al.  Micro total analysis systems. 2. Analytical standard operations and applications. , 2002, Analytical chemistry.

[3]  Kristen L. Helton,et al.  Microfluidic Overview of Global Health Issues Microfluidic Diagnostic Technologies for Global Public Health , 2006 .

[4]  Jin-Woo Choi,et al.  Disposable smart lab on a chip for point-of-care clinical diagnostics , 2004, Proceedings of the IEEE.

[5]  Darwin R. Reyes,et al.  Micro total analysis systems. 1. Introduction, theory, and technology. , 2002, Analytical chemistry.

[6]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[7]  T. Suzuki,et al.  Interaction of hepatitis B surface antigen with serum albumin of various species on polystylene latex particles , 2004, Medical Microbiology and Immunology.

[8]  S. Shoji.  Micro Total Analysis Systems , 1999 .

[9]  W. Bains.  Simple DNA probe assays based on particle agglutination. , 1998, Clinical chemistry.

[10]  Replications and analysis of microlens array fabricated by a modified LIGA process , 2006 .

[11]  Dong Sung Kim,et al.  Improved serpentine laminating micromixer with enhanced local advection , 2008 .

[12]  Bong-Kee Lee,et al.  Replication Quality of Flow-Through Microfilters in Microfluidic Lab-on-a-Chip for Blood Typing by Microinjection Molding , 2008 .

[13]  C Gärtner,et al.  Polymer microfabrication methods for microfluidic analytical applications , 2000, Electrophoresis.

[14]  Jijun Xiong,et al.  Passive valves based on hydrophobic microfluidics , 2003 .

[15]  A novel microplate agglutination method for blood grouping and reverse typing without the need for centrifugation , 2001, Transfusion.

[16]  V. Chaudhary,et al.  Whole-Blood Agglutination Assay for On-Site Detection of Human Immunodeficiency Virus Infection , 2003, Journal of Clinical Microbiology.

[17]  Dong Sung Kim,et al.  A serpentine laminating micromixer combining splitting/recombination and advection. , 2005, Lab on a chip.

[18]  Dong Sung Kim,et al.  Disposable integrated microfluidic biochip for blood typing by plastic microinjection moulding. , 2006, Lab on a chip.