Mechanotransduction In Vivo by Repeated Talin Stretch-Relaxation Events Depends upon Vinculin

The focal adhesion protein talin undergoes cycles of stretching and relaxation in living cells, suggesting a role in the transduction of mechanical into biochemical signals.

[1]  G. Schett,et al.  Mechanical stress-induced heat shock protein 70 expression in vascular smooth muscle cells is regulated by Rac and Ras small G proteins but not mitogen-activated protein kinases. , 2000, Circulation research.

[2]  M. Sheetz,et al.  Talin depletion reveals independence of initial cell spreading from integrin activation and traction , 2008, Nature Cell Biology.

[3]  David J Odde,et al.  Traction Dynamics of Filopodia on Compliant Substrates , 2008, Science.

[4]  Benoit Ladoux,et al.  Cytoskeletal coherence requires myosin-IIA contractility , 2010, Journal of Cell Science.

[5]  M. Sheetz,et al.  Force‐dependent integrin–cytoskeleton linkage formation requires downregulation of focal complex dynamics by Shp2 , 2003, The EMBO journal.

[6]  M. Sheetz,et al.  Local force and geometry sensing regulate cell functions , 2006, Nature Reviews Molecular Cell Biology.

[7]  W. Goldmann,et al.  Native talin is a dumbbell-shaped homodimer when it interacts with actin. , 1994, Journal of structural biology.

[8]  John F. Canny,et al.  A Computational Approach to Edge Detection , 1986, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[9]  Michael P. Sheetz,et al.  Force transduction by Triton cytoskeletons , 2002, The Journal of cell biology.

[10]  Brett L. Kutscher,et al.  A Conformational Switch in Vinculin Drives Formation and Dynamics of a Talin-Vinculin Complex at Focal Adhesions* , 2006, Journal of Biological Chemistry.

[11]  Jake M. Hofman,et al.  Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow. , 2006, Biophysical journal.

[12]  Viola Vogel,et al.  How Force Might Activate Talin's Vinculin Binding Sites: SMD Reveals a Structural Mechanism , 2008, PLoS Comput. Biol..

[13]  Andrés J. García,et al.  Contractility modulates cell adhesion strengthening through focal adhesion kinase and assembly of vinculin‐containing focal adhesions , 2010, Journal of cellular physiology.

[14]  M. Sheetz,et al.  Periodic Lamellipodial Contractions Correlate with Rearward Actin Waves , 2004, Cell.

[15]  Taekjip Ha,et al.  Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics , 2010, Nature.

[16]  Yasuhiro Sawada,et al.  Activation of a signaling cascade by cytoskeleton stretch. , 2004, Developmental cell.

[17]  A. Cumano,et al.  Forced Unfolding of Proteins Within Cells , 2007 .

[18]  Gaudenz Danuser,et al.  Differential Transmission of Actin Motion Within Focal Adhesions , 2007, Science.

[19]  Donald E Ingber,et al.  Investigating complexity of protein-protein interactions in focal adhesions. , 2008, Biochemical and biophysical research communications.

[20]  L. Lucy An iterative technique for the rectification of observed distributions , 1974 .

[21]  David R Critchley,et al.  Biochemical and structural properties of the integrin-associated cytoskeletal protein talin. , 2009, Annual review of biophysics.

[22]  Roger D Kamm,et al.  Force-induced activation of talin and its possible role in focal adhesion mechanotransduction. , 2007, Journal of biomechanics.

[23]  Marc Tramier,et al.  Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition. , 2009, Biophysical journal.

[24]  Kristopher E Kubow,et al.  Fibronectin forms the most extensible biological fibers displaying switchable force-exposed cryptic binding sites , 2009, Proceedings of the National Academy of Sciences.

[25]  Michael P. Sheetz,et al.  Force Sensing by Mechanical Extension of the Src Family Kinase Substrate p130Cas , 2006, Cell.

[26]  M. Sheetz,et al.  Force propagation across cells: mechanical coherence of dynamic cytoskeletons. , 2009, Current opinion in cell biology.

[27]  William H. Richardson,et al.  Bayesian-Based Iterative Method of Image Restoration , 1972 .

[28]  K. Rottner,et al.  The Vinculin-ΔIn20/21 Mouse: Characteristics of a Constitutive, Actin-Binding Deficient Splice Variant of Vinculin , 2010, PloS one.

[29]  Gaudenz Danuser,et al.  Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed , 2008, The Journal of cell biology.

[30]  Michael P. Sheetz,et al.  Talin1 is critical for force-dependent reinforcement of initial integrin–cytoskeleton bonds but not tyrosine kinase activation , 2003, The Journal of cell biology.

[31]  Michael P. Sheetz,et al.  Stretching Single Talin Rod Molecules Activates Vinculin Binding , 2009, Science.

[32]  Michael W. Davidson,et al.  Nanoscale architecture of integrin-based cell adhesions , 2010, Nature.

[33]  J. Klafter,et al.  A Role for the Juxtamembrane Cytoplasm in the Molecular Dynamics of Focal Adhesions , 2009, PloS one.