Design and Preparation of a Microfluidic System for Stretching of Cells in Topographic Microstructures

In this work, a new method was proposed to construct an elastic membrane with specially designed micropillar arrays and integrate the membrane into a microfluidic chip. RFP-transfected human mesenchymal stem cells were seeded in the micropillar arrays. Cyclic stretching of cells in the topographic microstructures resulted in different cell activities (cell spreading, orientation, and migration). The cell activities were enhanced with a proper level of spatial constraint on cells. This study explored a new approach to spatially influence cell behaviors.

[1]  Jiandong Ding,et al.  A simplified yet enhanced and versatile microfluidic platform for cyclic cell stretching on an elastic polymer , 2020, Biofabrication.

[2]  C. Kong,et al.  Maturation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) in 3D collagen matrix: Effects of niche cell supplementation and mechanical stimulation. , 2017, Acta biomaterialia.

[3]  E. Danen,et al.  A guide to mechanobiology: Where biology and physics meet. , 2015, Biochimica et biophysica acta.

[4]  Jianping Fu,et al.  Forcing stem cells to behave: a biophysical perspective of the cellular microenvironment. , 2012, Annual review of biophysics.

[5]  Alberto Avolio,et al.  Mechanical stretch: physiological and pathological implications for human vascular endothelial cells , 2015, Vascular cell.

[6]  Benjamin Geiger,et al.  Cell reorientation under cyclic stretching , 2014, Nature Communications.

[7]  S. Kook,et al.  Cyclic mechanical stretch stimulates the proliferation of C2C12 myoblasts and inhibits their differentiation via prolonged activation of p38 MAPK. , 2008, Molecules and cells.

[8]  R. Kaunas,et al.  Dependence of cyclic stretch-induced stress fiber reorientation on stretch waveform. , 2012, Journal of biomechanics.

[9]  Hossein Baharvand,et al.  Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro. , 2006, The International journal of developmental biology.

[10]  Michael Sheetz,et al.  Cyclic stretching of soft substrates induces spreading and growth , 2015, Nature Communications.

[11]  Sungbo Cho,et al.  Detection of the osteogenic differentiation of mesenchymal stem cells in 2D and 3D cultures by electrochemical impedance spectroscopy. , 2010, Journal of biotechnology.

[12]  M. Shokrgozar,et al.  Cyclic Stretch Effects on Adipose-Derived Stem Cell Stiffness, Morphology and Smooth Muscle Cell Gene Expression , 2017, Tissue Engineering and Regenerative Medicine.

[13]  Huajian Gao,et al.  Two characteristic regimes in frequency-dependent dynamic reorientation of fibroblasts on cyclically stretched substrates. , 2008, Biophysical journal.

[14]  A. Abbas,et al.  Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells , 2019, Stem cells international.

[15]  Timothy M Maul,et al.  Mechanical stimulation induces morphological and phenotypic changes in bone marrow-derived progenitor cells within a three-dimensional fibrin matrix. , 2007, Journal of biomedical materials research. Part A.

[16]  Il Keun Kwon,et al.  Mechanical stretching for tissue engineering: two-dimensional and three-dimensional constructs. , 2012, Tissue engineering. Part B, Reviews.

[17]  Jiandong Ding,et al.  Critical Frequency and Critical Stretching Rate for Reorientation of Cells on a Cyclically Stretched Polymer in a Microfluidic Chip. , 2021, ACS applied materials & interfaces.

[18]  Candice Sears,et al.  The many ways adherent cells respond to applied stretch. , 2016, Journal of biomechanics.

[19]  Jennifer H. Elisseeff,et al.  Mimicking biological functionality with polymers for biomedical applications , 2016, Nature.

[20]  Benjamin J Ellis,et al.  Effect of mechanical boundary conditions on orientation of angiogenic microvessels. , 2008, Cardiovascular research.

[21]  B. Ladoux,et al.  Mechanobiology of collective cell behaviours , 2017, Nature Reviews Molecular Cell Biology.

[22]  Jiandong Ding,et al.  Cell–Material Interactions Revealed Via Material Techniques of Surface Patterning , 2013, Advanced materials.

[23]  P. Dartsch,et al.  Response of cultured endothelial cells to mechanical stimulation , 1989, Basic Research in Cardiology.