Early postoperative changes in hematological, erythrocyte aggregation and blood coagulation parameters after unilateral implantation of polytetrafluoroethylene vascular graft in the femoral artery of beagle dogs.

PURPOSE The failure of small-caliber vascular grafts still means a serious problem. Concerning the early postoperative complications we aimed to investigate the hemostaseological and hemorheological aspects of this issue in a canine model. METHODS In the Control group only anesthesia was induced. In the Grafted group under general anesthesia a 3.5-cm segment was resected unilaterally from the femoral artery and replaced with a PTFE graft (diameter: 3 mm). On the 1st-3rd-5th-7th and 14th postoperative days the skin temperature of both hind limbs was measured, and blood sampling occurred for hematological, hemostaseological and hemorheological tests. RESULTS The skin temperature of the operated versus intact limbs did not differ. In the Grafted group leukocyte count was elevated by the 1st postoperative day, while platelet count increased over the entire follow-up period. Fibrinogen concentration rose on the 1st-5th days, activated partial thromboplastin time increased on the 3rd-7th days. Erythrocyte aggregation was enhanced significantly on the 1st-5th days. In specimens taken on the 14th day, histologically we found matured thrombus narrowing the graft lumen. CONCLUSIONS Small-caliber PTFE graft implantation into the femoral artery caused significant changes in several hemostaseological and hemorheological parameters. However, better clarifying the factors leading to early thrombosis of these grafts needs further studies.

[1]  N. Németh,et al.  Following-up changes in red blood cell deformability and membrane stability in the presence of PTFE graft implanted into the femoral artery in a canine model , 2014, Korea-Australia Rheology Journal.

[2]  N. Németh,et al.  Hemorheological changes in ischemia-reperfusion: an overview on our experimental surgical data. , 2014, Clinical hemorheology and microcirculation.

[3]  X. Mo,et al.  Heparin loading and pre-endothelialization in enhancing the patency rate of electrospun small-diameter vascular grafts in a canine model. , 2013, ACS applied materials & interfaces.

[4]  C. Saldanha.  Fibrinogen interaction with the red blood cell membrane. , 2013, Clinical hemorheology and microcirculation.

[5]  J. Stoltz,et al.  Progress in vascular graft substitute. , 2013, Clinical hemorheology and microcirculation.

[6]  Yasuhiro Nakamura,et al.  Endovascular aneurysmal models at the external iliac artery of dogs. , 2012, Journal of vascular surgery.

[7]  E. Rőth,et al.  Histological Examination of Vascular Lesions Caused by Stent Implantation in Humans and in Comparative Experimental Animal Model , 2012, Acta biologica Hungarica.

[8]  E. Ingham,et al.  The Use of Antithrombotic Therapies in Reducing Synthetic Small-Diameter Vascular Graft Thrombosis , 2012, Vascular and endovascular surgery.

[9]  S. Haulon,et al.  Safety, healing, and efficacy of vascular prostheses coated with hydroxypropyl-β-cyclodextrin polymer: experimental in vitro and animal studies. , 2012, European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery.

[10]  Silvio Romero de Barros-Marques,et al.  Sugarcane biopolymer patch in femoral vein angioplasty on dogs. , 2012, Journal of vascular surgery.

[11]  A. Davies,et al.  Chapter IV: Treatment of critical limb ischaemia. , 2011, European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery.

[12]  Oguz K. Baskurt,et al.  Red Blood Cell Aggregation , 2011 .

[13]  M. Alley,et al.  Hemodynamic comparison of differing anastomotic geometries using magnetic resonance velocimetry. , 2011, The Journal of surgical research.

[14]  E. Minar,et al.  Claudication: treatment options for femoropopliteal disease. , 2011, Progress in cardiovascular diseases.

[15]  Jielu Yan,et al.  Effect of postoperative fractionated radiotherapy on canine ePTFE graft neointima and anastomotic stoma healing: a preliminary experimental study. , 2011, Asian journal of surgery.

[16]  Narutoshi Hibino,et al.  Vascular tissue engineering: towards the next generation vascular grafts. , 2011, Advanced drug delivery reviews.

[17]  J. Feijen,et al.  Tissue engineering of small-diameter vascular grafts: a literature review. , 2011, Clinical hemorheology and microcirculation.

[18]  R. Ceneviva,et al.  Morphologic evaluation of the use of a latex prosthesis in videolaparoscopic inguinoplasty: an experimental study in dogs. , 2011, Acta cirurgica brasileira.

[19]  S. Chien,et al.  Effects of disturbed flow on vascular endothelium: pathophysiological basis and clinical perspectives. , 2011, Physiological reviews.

[20]  H. Takagi,et al.  A contemporary meta-analysis of Dacron versus polytetrafluoroethylene grafts for femoropopliteal bypass grafting. , 2010, Journal of vascular surgery.

[21]  A. Chandra,et al.  When should open surgery be the initial option for critical limb ischaemia? , 2010, European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery.

[22]  N. Németh,et al.  Hematological and hemostaseological alterations after warm and cold limb ischemia-reperfusion in a canine model. , 2009, Acta cirurgica brasileira.

[23]  J. Beard.  Which is the best revascularization for critical limb ischemia: Endovascular or open surgery? , 2008, Journal of vascular surgery.

[24]  O. Baskurt.  In vivo correlates of altered blood rheology. , 2008, Biorheology.

[25]  S. Lyden,et al.  Current trends in lower extremity revascularization. , 2007, The Surgical clinics of North America.

[26]  M. Matsushita,et al.  Healing of implanted expanded polytetrafluoroethylene vascular access grafts with different internodal distances: a histologic study in dogs. , 2004, European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery.

[27]  U. Settmacher,et al.  Local haemodynamics and shear stress in cuffed and straight PTFE-venous anastomoses: an in-vitro comparison using particle image velocimetry. , 2003, European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery.

[28]  M. Silver,et al.  Femoropopliteal occlusive disease: Diagnosis, indications for treatment, and results of interventional therapy , 2002, Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions.

[29]  C. Jerusalem,et al.  Patency and morphology of fibrous polyurethane vascular prostheses implanted in the femoral artery of dogs after seeding with subcultivated endothelial cells. , 1993, European journal of vascular surgery.

[30]  C. Jerusalem,et al.  Development and long-term fate of a cellular lining in fibrous polyurethane vascular prostheses implanted in the dog carotid and femoral artery. A scanning and light microscopical study up to 53 months after implantation. , 1992, The Journal of cardiovascular surgery.

[31]  J C Stanley,et al.  Small-caliber polyurethane and polytetrafluoroethylene grafts: a comparative study in a canine aortoiliac model. , 1990, Journal of biomedical materials research.

[32]  F. W. Blaisdell,et al.  Why small caliber vascular grafts fail: a review of clinical and experimental experience and the significance of the interaction of blood at the interface. , 1986, The Journal of surgical research.

[33]  M. Reidy,et al.  Mechanisms of arterial graft healing. Rapid transmural capillary ingrowth provides a source of intimal endothelium and smooth muscle in porous PTFE prostheses. , 1986, The American journal of pathology.