Effect of different carbon sources on the accumulation of carbohydrate, nutrient absorption and the survival rate of Chinese Ash (Fraxinus mandshurica) explants in vitro

In this study, we analyzed the effect of different carbon sources on: (i) carbohydrate and nutrient absorption in Chinese Ash (Fraxinus mandshurica) explants in vitro, and (ii) explant survival rate during ex vitroacclimatization. The explants were cultured under one of five conditions: (1) in a solid medium supplemented with 30 g l-1 sucrose (SM); (2) in a modified temporary immersion bioreactor (TIB) supplemented with sucrose at 0 (TIB0) or (3) 30 g l-1 (TIBS) concentration; (4) in TIB flushed with CO2 at 700 to 900 µl l-1 (TIBC) with no sucrose; or in TIB flushed with CO2 at 700 to 900 µl/l while simultaneously adding sucrose 30 g l-1 (TIBCS). The explants cultured under TIBCS exhibited the highest level of root induction and acclimatization survival rate. In addition, these explants exhibited maximized fresh weights (FW), dry weights (DW), and sucrose contents. Explants cultured in SM showed the highest rate of carbohydrate accumulation. At the end of the culture incubation period, absorption of nitrogen, phosphorus, potassium, magnesium, iron, zinc and manganese was also higher in explants cultured in SM than in TIB. This study revealed that the use of TIBCS provided optimum explant survival.   Key words: Fraxinus mandshurica, temporary immersion bioreactor, CO2 enrichment, nutrient absorption.

[1]  M. Cañal,et al.  CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson) , 2010, In Vitro Cellular & Developmental Biology - Plant.

[2]  Yulan Xiao,et al.  Growth and Photosynthetic Capability of Momordica grosvenori Plantlets Grown Photoautotrophically in Response to Light Intensity , 2009 .

[3]  W. Lihua,et al.  Effects of plant growth regulators on growth and endogenous hormones of in vitro plantlets of Manchurian ash. , 2009 .

[4]  L. Incoll,et al.  The control of leaf photosynthesis rate by the level of assimilate concentration in the leaf: A review of the hypothesis , 1968, The Botanical Review.

[5]  T. Fujiwara,et al.  Physiological roles and transport mechanisms of boron: perspectives from plants , 2008, Pflügers Archiv - European Journal of Physiology.

[6]  Inaudis Cejas,et al.  Photosynthesis and carbon metabolism in plantain (Musa AAB) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization , 2005, In Vitro Cellular & Developmental Biology - Plant.

[7]  M. Cañal,et al.  The effect of headspace renewal in a Temporary Immersion Bioreactor on plantain (Musa AAB) shoot proliferation and quality , 2006, Plant Cell Tissue and Organ Culture.

[8]  M. Jackson.  Aeration stress in plant tissue cultures , 2005 .

[9]  F. Blakeway,et al.  Use of the temporary immersion bioreactor system (RITA®) for production of commercial Eucalyptus clones in Mondi Forests (SA) , 2005, Plant Cell, Tissue and Organ Culture.

[10]  M. Lartaud,et al.  Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos , 2005, Plant Cell, Tissue and Organ Culture.

[11]  T. Kozai,et al.  Photoautotrophic and photomixotrophic growth of strawberry plantlets in vitro and changes in nutrient composition of the medium , 1991, Plant Cell, Tissue and Organ Culture.

[12]  M. Stitt,et al.  Control of photosynthetic sucrose synthesis by fructose-2,6-bisphosphate , 1985, Planta.

[13]  Alain Pareilleux,et al.  Mass cultivation of Medicago sativa growing on lactose: Kinetic aspects , 1981, European journal of applied microbiology and biotechnology.

[14]  Y. Desjardins,et al.  Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets , 2004, Plant Cell, Tissue and Organ Culture.

[15]  Tan Yan.  Tissue Culture and Plantlet Regeneration from Hypocotyl of Fraxinus mandshurica , 2003 .

[16]  Sandra B. Wilson,et al.  A Forced Ventilation Micropropagation System for Photoautotrophic Production of Sweetpotato Plug Plantlets in a Scaled-up Culture Vessel: I. Growth and Uniformity , 2001 .

[17]  Sandra B. Wilson,et al.  A Forced Ventilation Micropropagation System for Photoautotrophic Production of Sweetpotato Plug Plantlets in a Scaled-up Culture Vessel: II. Carbohydrate Status , 2001 .

[18]  Maritza Escalona,et al.  Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems , 1999, Plant Cell Reports.

[19]  P. Debergh,et al.  Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micropropagated plants , 1998 .

[20]  P. Debergh,et al.  Impact of sugar concentration in vitro on photosynthesis and carbon metabolism during ex vitro acclimatization of Spathiphyllum plantlets , 1996 .

[21]  J. Huylenbroeck,et al.  Sugar and starch metabolism during ex vitro rooting and acclimatization of micropropagated Spathiphyllum ‘Petite’ plantlets , 1995 .

[22]  C. Leifert,et al.  Effect of Mineral Nutrition on the Growth and Multiplication of in vitro Cultured Plants , 1990 .

[23]  S. Huber,et al.  Changes in Starch Formation and Activities of Sucrose Phosphate Synthase and Cytoplasmic Fructose-1,6-bisphosphatase in Response to Source-Sink Alterations. , 1983, Plant physiology.

[24]  B. McCown,et al.  Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture , 1980 .