A Multicentre Study of Acute In Vitro Cytotoxicity in Rat Hepatocytes: Tentative Correlation Between In Vitro Toxicities and In Vivo Data

A multicentre validation study of the acute in vitro cytotoxicities of 31 liquid or solid chemicals was carried out by six laboratories, using primary rat hepatocyte cultures as a model system. We report here a comparison of neutral red uptake IC50 and LD50 values. Oral, i.p. and i.v. LD50 values were available for 27, 24 and 18 chemicals, respectively, and an IC50 value was obtained for 15, 14 and 11 of these compounds, respectively. A significant correlation was found only between IC50 and i.v. LD50 values.

[1]  J P Cano,et al.  A multicentre study of acute in vitro cytotoxicity in rat liver cells. , 1991, Toxicology in vitro : an international journal published in association with BIBRA.

[2]  M. Garle,et al.  Correlation of acute lethal potency with in vitro cytotoxicity. , 1990, Toxicology in vitro : an international journal published in association with BIBRA.

[3]  M. Balls,et al.  Comparison of the in vitro cytotoxicities and acute in vivo toxicities of 59 chemicals. , 1987, Molecular toxicology.

[4]  B. Ekwall.  Correlation between cytotoxicity in vitro and LD50-values. , 2009, Acta pharmacologica et toxicologica.