Embryogenic cell suspensions (ECS) of 'Grande Naine' (Musa AAA group) were plated on RD1 or M3 medium for the regeneration of somatic embryos, 1 to 2 weeks after last subculture. The first regenerable somatic embryos were observed about 3 weeks after inoculation. After 8 weeks of culture, the mass of the embryogenic mass increased about 5 to 18 times and the number of somatic embryos that could be regenerated from 1 mL settled cell volume (SCV) of ECS ranged between 0.71×10~5 and 3.07×10~5, depending on pre-culture time in liquid medium before regeneration, regeneration media and incubation conditions (light/dark). The frequency of plant recovery and the amount of plantlets regenerated from 1 mL SCV of ECS were indirectly affected by somatic embryos regeneration conditions that were studied.