SERS-fluorescence bimodal nanoprobes for in vitro imaging of fatty acid responsive receptor GPR120.

G-protein-coupled receptor 120 (GPR120), as a member of the rhodopsin family of G-protein-coupled receptors, has been shown to function as a sensor for dietary fat in the gustatory and digestive systems. Its specific role in the chemoreception of fatty acids, which is thought to be crucial in understanding the mechanism surrounding the control of fat intake and, accordingly, in the treatment of obesity, remains unclear. Here we report a novel surface-enhanced Raman spectroscopy (SERS)-fluorescence bimodal microscopic technique for detection and imaging of GPR120 in single living cells. CaMoO4:Eu3+@AuNR hybrid nanoparticles are synthesized and characterized as imaging probes. Biocompatibility and imaging capability of the probes are investigated using a model HEK293 cell line with an inducible GPR120 gene transfection. Cellular distribution of GPR120 is visualized by single-cell SERS and fluorescence imaging. A dose-dependent GPR120 response to linoleic acid treatment is revealed by SERS.

[1]  Homan Kang,et al.  Near‐Infrared SERS Nanoprobes with Plasmonic Au/Ag Hollow‐Shell Assemblies for In Vivo Multiplex Detection , 2013 .

[2]  Jayaram Chandrashekar,et al.  A Novel Family of Mammalian Taste Receptors , 2000, Cell.

[3]  A. Moore,et al.  Noninvasive MRI-SERS imaging in living mice using an innately bimodal nanomaterial. , 2011, ACS nano.

[4]  N. Khan,et al.  Taste of Fat: A Sixth Taste Modality? , 2016, Physiological reviews.

[5]  Li Wang,et al.  Nuclear targeted nanoprobe for single living cell detection by surface-enhanced Raman scattering. , 2009, Bioconjugate chemistry.

[6]  A. Rosato,et al.  Magneto-plasmonic Au-Fe alloy nanoparticles designed for multimodal SERS-MRI-CT imaging. , 2014, Small.

[7]  Eun Kyu Lee,et al.  Fabrication of SERS-fluorescence dual modal nanoprobes and application to multiplex cancer cell imaging. , 2012, Nanoscale.

[8]  Neil Mann,et al.  Origins and evolution of the Western diet: health implications for the 21st century. , 2005, The American journal of clinical nutrition.

[9]  J. Zhao,et al.  Controlled plasmonic nanostructures for surface-enhanced spectroscopy and sensing. , 2008, Accounts of chemical research.

[10]  Anhong Zhou,et al.  Non-invasive detection of biomechanical and biochemical responses of human lung cells to short time chemotherapy exposure using AFM and confocal Raman spectroscopy , 2013 .

[11]  Hassimi Sadou,et al.  Ca2+ signaling in taste bud cells and spontaneous preference for fat: unresolved roles of CD36 and GPR120. , 2014, Biochimie.

[12]  S. Rai,et al.  Enhanced photoluminescence in CaMoO4:Eu3+ by Gd3+ co-doping. , 2014, Dalton transactions.

[13]  J. Bukowska,et al.  Surface‐enhanced Raman scattering (SERS) of 4‐mercaptobenzoic acid on silver and gold substrates , 2003 .

[14]  Tuan Vo-Dinh,et al.  SERS-based plasmonic nanobiosensing in single living cells , 2009, Analytical and bioanalytical chemistry.

[15]  Martin A. B. Hedegaard,et al.  Laterally resolved and direct spectroscopic evidence of nanometer-sized lipid and protein domains on a single cell. , 2011, Small.

[16]  Salvador Tomas,et al.  Modulation of in-membrane receptor clustering upon binding of multivalent ligands. , 2013, Journal of the American Chemical Society.

[17]  Elizabeth Vargis,et al.  In vitro biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug. , 2015, Analytical methods : advancing methods and applications.

[18]  Royston Goodacre,et al.  Characterisation and identification of bacteria using SERS. , 2008, Chemical Society reviews.

[19]  Toshihiro Hashimoto,et al.  CD36- and GPR120-mediated Ca²⁺ signaling in human taste bud cells mediates differential responses to fatty acids and is altered in obese mice. , 2014, Gastroenterology.

[20]  Zachary D. Schultz,et al.  Selective Detection of RGD-Integrin Binding in Cancer Cells Using Tip Enhanced Raman Scattering Microscopy. , 2016, Analytical chemistry.

[21]  Yuancheng Li,et al.  Facile non-hydrothermal synthesis of oligosaccharides coated sub-5 nm magnetic iron oxide nanoparticles with dual MRI contrast enhancement effect. , 2014, Journal of materials chemistry. B.

[22]  S. Rai,et al.  Influence of Gd3+ co-doping on structural property of CaMoO4:Eu nanoparticles. , 2014, Dalton transactions.

[23]  Lifu Xiao,et al.  Label‐free and non‐invasive monitoring of porcine trophoblast derived cells: differentiation in serum and serum‐free media , 2015, Journal of biophotonics.

[24]  Robert F Margolskee,et al.  Molecular Mechanisms of Bitter and Sweet Taste Transduction* , 2002, The Journal of Biological Chemistry.

[25]  W. Fang,et al.  pH-controllable drug carrier with SERS activity for targeting cancer cells. , 2014, Biosensors & bioelectronics.

[26]  Michael S. Feld,et al.  Surface-Enhanced Raman Spectroscopy in Single Living Cells Using Gold Nanoparticles , 2002 .

[27]  L. Johnston,et al.  Nanoscale Imaging of Epidermal Growth Factor Receptor Clustering , 2009, The Journal of Biological Chemistry.

[28]  Zachary D. Schultz,et al.  Probing Membrane Receptor–Ligand Specificity with Surface- and Tip- Enhanced Raman Scattering , 2017, Analytical chemistry.

[29]  N Rifai,et al.  Association between dietary patterns and plasma biomarkers of obesity and cardiovascular disease risk. , 2001, The American journal of clinical nutrition.

[30]  Lingxin Chen,et al.  Biocompatible triplex Ag@SiO2@mTiO2 core-shell nanoparticles for simultaneous fluorescence-SERS bimodal imaging and drug delivery. , 2012, Chemistry.

[31]  A. Parchur,et al.  Near-infrared photothermal therapy of Prussian-blue-functionalized lanthanide-ion-doped inorganic/plasmonic multifunctional nanostructures for the selective targeting of HER2-expressing breast cancer cells. , 2016, Biomaterials science.

[32]  S. Rai,et al.  Luminescence properties of Tb3+-doped CaMoO4 nanoparticles: annealing effect, polar medium dispersible, polymer film and core-shell formation. , 2012, Dalton transactions.

[33]  Sanjiv S. Gambhir,et al.  Multiplexed imaging of surface enhanced Raman scattering nanotags in living mice using noninvasive Raman spectroscopy , 2009, Proceedings of the National Academy of Sciences.

[34]  N. Shah,et al.  Surface-enhanced Raman spectroscopy. , 2008, Annual review of analytical chemistry.

[35]  Lingxin Chen,et al.  Upconversion fluorescence-SERS dual-mode tags for cellular and in vivo imaging. , 2014, ACS applied materials & interfaces.

[36]  R. Dasari,et al.  Single Molecule Detection Using Surface-Enhanced Raman Scattering (SERS) , 1997 .

[37]  Céline Martin,et al.  The Lipid-Sensor Candidates CD36 and GPR120 Are Differentially Regulated by Dietary Lipids in Mouse Taste Buds: Impact on Spontaneous Fat Preference , 2011, PloS one.

[38]  John Paul Pezacki,et al.  Development of nanoparticle probes for multiplex SERS imaging of cell surface proteins. , 2010, Nanoscale.

[39]  T. Liou,et al.  Toxicity effects of short term diesel exhaust particles exposure to human small airway epithelial cells (SAECs) and human lung carcinoma epithelial cells (A549). , 2012, Toxicology letters.

[40]  Malini Olivo,et al.  Ultrasensitive near-infrared Raman reporters for SERS-based in vivo cancer detection. , 2011, Angewandte Chemie.

[41]  Jian Xu,et al.  Single cell Raman spectroscopy for cell sorting and imaging. , 2012, Current opinion in biotechnology.

[42]  Steven R. Emory,et al.  Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering , 1997, Science.

[43]  Sami Damak,et al.  Taste Preference for Fatty Acids Is Mediated by GPR40 and GPR120 , 2010, The Journal of Neuroscience.

[44]  S. C. Gadkari,et al.  Luminescence properties of Eu3+ doped CaMoO4 nanoparticles. , 2011, Dalton transactions.

[45]  D. McLean,et al.  Automated Autofluorescence Background Subtraction Algorithm for Biomedical Raman Spectroscopy , 2007, Applied spectroscopy.

[46]  Ramasamy Manoharan,et al.  Detection and identification of a single DNA base molecule using surface-enhanced Raman scattering (SERS) , 1998 .

[47]  Hongxing Xu,et al.  Spectroscopy of Single Hemoglobin Molecules by Surface Enhanced Raman Scattering , 1999 .

[48]  Hong-Wu Tang,et al.  Probing intrinsic and extrinsic components in single osteosarcoma cells by near-infrared surface-enhanced Raman scattering. , 2007, Analytical chemistry.

[49]  Wang Li,et al.  SERS-fluorescence joint spectral encoding using organic-metal-QD hybrid nanoparticles with a huge encoding capacity for high-throughput biodetection: putting theory into practice. , 2012, Journal of the American Chemical Society.

[50]  James Nyagilo,et al.  Gold nanotags for combined multi-colored Raman spectroscopy and x-ray computed tomography , 2010, Nanotechnology.

[51]  L. Santen,et al.  Cargo binding promotes KDEL receptor clustering at the mammalian cell surface , 2016, Scientific Reports.

[52]  Lifu Xiao,et al.  Imaging of epidermal growth factor receptor on single breast cancer cells using surface-enhanced Raman spectroscopy. , 2014, Analytica chimica acta.