Differential cold-shock resistance among acclimated European mussel populations

To study differential cold-shock resistance of marine mussel populations (Mytilus spp.) from different climatic regions in Europe, we sampled 12 populations, ranging from 43 to 58°N. Minimum critical temperatures for aerobic metabolism (CTmin) were determined before and after 3 months of common acclimatization in an outdoor mesocosm. Additionally, chill coma in response to cold shock was used to test for differences in physiological plasticity between the translocated populations. The CTmin followed a steep cline, being positively related to the ambient temperatures before translocation (p < 0.0001), and became similar between populations after 3 months in the outdoor mesocosms (p > 0.05). Differential chill coma responses separated the populations into two groups that were also geographically separated by the English Channel. The southern populations showed a much stronger and faster sensitivity to chill than the northern populations, indicating differential physiological adaptation between the two groups. The results are discussed in relation to the genetic background and climatic isolation of the populations.

[1]  D. Schiedek,et al.  Patterns of organic osmolytes in two marine bivalves, Macoma balthica, and Mytilus spp., along their European distribution , 2006 .

[2]  H. Pörtner,et al.  Energy metabolism and ATP free-energy change of the intertidal wormSipunculus nudus below a critical temperature , 1996, Journal of Comparative Physiology B.

[3]  E. Gosling,et al.  Population genetic structure of mussels from the Baltic Sea , 1988, Helgoländer Meeresuntersuchungen.

[4]  H. Jenner,et al.  Factors influencing the upper temperature tolerances of three mussel species in a brackish water canal: Size, season and laboratory protocols , 2005, Biofouling.

[5]  C. Cunningham,et al.  INVITED REVIEW: Local adaptation and species segregation in two mussel (Mytilus edulis × Mytilus trossulus) hybrid zones , 2004, Molecular ecology.

[6]  K. Reise,et al.  Introduced Pacific oysters (Crassostrea gigas) in the northern Wadden Sea: invasion accelerated by warm summers? , 2005, Helgoland Marine Research.

[7]  V. Debat,et al.  Cold adaptation in geographical populations of Drosophila melanogaster: phenotypic plasticity is more important than genetic variability , 2004 .

[8]  V. Borutaite,et al.  Inhibition of mitochondrial respiratory complex I by nitric oxide, peroxynitrite and S-nitrosothiols. , 2004, Biochimica et biophysica acta.

[9]  M. Wołowicz,et al.  Mitochondrial DNA lineages in the European populations of mussels (Mytilus spp.) , 2004 .

[10]  Peter I. Miller,et al.  Hydrographical variability on the French continental shelf in the Bay of Biscay, during the 1990s , 2004 .

[11]  M. Staurnes.  Effects of acute temperature decreases on turbot fry and juveniles , 1994, Aquaculture International.

[12]  F. Bozinovic,et al.  Adaptive latitudinal shifts in the thermal physiology of a terrestrial isopod , 2004 .

[13]  M. Wonham.  MINI-REVIEW: DISTRIBUTION OF THE MEDITERRANEAN MUSSEL MYTILUS GALLOPROVINCIALIS (BIVALVIA: MYTILIDAE) AND HYBRIDS IN THE NORTHEAST PACIFIC , 2004 .

[14]  N. Knowlton.  Molecular genetic analyses of species boundaries in the sea , 2000, Hydrobiologia.

[15]  S. Chown,et al.  Temperature effects on locomotor activity rates of sub-Antarctic oribatid mites , 2004, Polar Biology.

[16]  P. David,et al.  Introgression patterns in the mosaic hybrid zone between Mytilus edulis and M. galloprovincialis , 2003, Molecular ecology.

[17]  P. Luttikhuizen,et al.  Mytilus galloprovincialis-type foot-protein-1 alleles occur at low frequency among mussels in the Dutch Wadden Sea , 2002 .

[18]  H. Pörtner,et al.  Climate variations and the physiological basis of temperature dependent biogeography: systemic to molecular hierarchy of thermal tolerance in animals. , 2002, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[19]  G. Somero.  Thermal Physiology and Vertical Zonation of Intertidal Animals: Optima, Limits, and Costs of Living1 , 2002, Integrative and comparative biology.

[20]  D. Hicks,et al.  Temperature acclimation of upper and lower thermal limits and freeze resistance in the nonindigenous brown mussel, Perna perna (L.), from the Gulf of Mexico , 2002 .

[21]  J. Qiu,et al.  Ontogenetic changes in hyposaline tolerance in the mussels Mytilus edulis and M. trossulus: implications for distribution , 2002 .

[22]  L. Peck,et al.  Metabolic Demand, Oxygen Supply, and Critical Temperatures in the Antarctic Bivalve Laternula elliptica , 2002, Physiological and Biochemical Zoology.

[23]  G. Baggerman,et al.  Cold stress alters Mytilus edulis pedal ganglia expression of mu opiate receptor transcripts determined by real-time RT-PCR and morphine levels. , 2002, Brain research. Molecular brain research.

[24]  R. Huey,et al.  Chill‐Coma Temperature in Drosophila: Effects of Developmental Temperature, Latitude, and Phylogeny , 2001, Physiological and Biochemical Zoology.

[25]  F. Bonhomme,et al.  The zone of sympatry and hybridization of Mytilus edulis and M. galloprovincialis, as described by intron length polymorphism at locus mac-1 , 2001, Heredity.

[26]  J. M. Elliott,et al.  Functional models for growth and food consumption of Atlantic salmon parr, Salmo salar, from a Norwegian river , 2001 .

[27]  Sebastiaan A.L.M. Kooijman,et al.  Dynamic Energy and Mass Budgets in Biological Systems , 2000 .

[28]  A. Meyer,et al.  Origin of the antitropical distribution pattern in marine mussels (Mytilus spp.): routes and timing of transequatorial migration , 2000 .

[29]  J. Geller.  Decline of a Native Mussel Masked by Sibling Species Invasion , 1999 .

[30]  G. Brown,et al.  Nitric oxide and mitochondrial respiration. , 1999, Biochimica et biophysica acta.

[31]  C. Koutsikopoulos,et al.  Temporal trends and spatial structures of the sea surface temperature in the Bay of Biscay , 1998 .

[32]  H. Pörtner,et al.  Temperature induced anaerobiosis in two populations of the polychaete worm Arenicola marina (L.) , 1997, Journal of Comparative Physiology B.

[33]  G. Somero,et al.  Interspecific variation in thermal denaturation of proteins in the congeneric musselsMytilus trossulus andM. galloprovincialis: evidence from the heat-shock response and protein ubiquitination , 1996 .

[34]  A. Zwaan,et al.  Anoxic or aerial survival of bivalves and other euryoxic invertebrates as a useful response to environmental stress—A comprehensive review , 1996 .

[35]  W. Bennett,et al.  Comparison of methods for determining low temperature tolerance: experiments with pinfish, Lagodon rhomboides , 1992 .

[36]  R. K. Koehn,et al.  Evolutionary genetics of the Mytilus edulis complex in the North Atlantic region , 1988 .

[37]  R. S. Scheltema.  On dispersal and planktonic larvae of benthic invertebrates: an eclectic overview and summary of problems , 1986 .

[38]  M. Sprung.  Physiological energetics of mussel larvae (Mytilus edulis). I. Shell growth and biomass , 1984 .

[39]  M. Sprung,et al.  Physiological energetics of mussel larvae (Mytilus edulis). II. Food uptake , 1984 .

[40]  D. Murphy.  Freezing resistance in intertidal invertebrates. , 1983, Annual review of physiology.

[41]  E. Bourget.  Seasonal variations of cold tolerance in intertidal mollusks and relation to environmental conditions in the St. Lawrence Estuary , 1983 .

[42]  C. Drewes,et al.  THE EFFECTS OF COOLING ON AN IDENTIFIED REFLEX PATHWAY IN THE COCKROACH (PERIPLANETA AMERICANA), IN RELATION TO CHILL-COMA , 1982 .

[43]  A. V. Aarset.  Freezing tolerance in intertidal invertebrates (a review) , 1982 .

[44]  S. Wright,et al.  Influence of temperature and unstirred layers on the kinetics of glycine transport in isolated gills of Mytilus californianus. , 1980, The Journal of experimental zoology.

[45]  R. Wallis.  Thermal tolerance of Mytilus edulis of Eastern Australia , 1975 .

[46]  J. Widdows,et al.  Temperature Acclimation of Mytilus Edulis With Reference to its Energy Budget , 1971, Journal of the Marine Biological Association of the United Kingdom.