2170 T-Cell Receptor Vf 3 Gene Expression in Infiltrating Cells in Murine Hearts With Acute Myocarditis Caused by Coxsackievirus B 3

Background In viral myocarditis, we previously reported that natural killer cells infiltrate the heart first, then activated T cells infiltrate second and play an important role in the pathogenesis of the myocardial damage. Methods and Resuls To elucidate the nature of T-cell infiltration, using a murine model of acute myocarditis caused by coxsackievirus B3, we analyzed the expression of T-cell receptor (TCR) V(3 genes in infiltrating cells in the heart by polymerase chain reaction (PCR). The PCR-amplified products were confirmed by Southern blot hybridization with a Cal cDNA probe. In contrast to spleen lymphocytes, the repertoire of VJ3 gene transcripts in the heart was restricted. The infiltrating cells expressing V(310 were found in six of eight hearts of mice with acute myocarditis. The infiltrating cells expressing V(88 and V(313 were found in four of eight hearts with myocarditis, respectively. Immunoperoxidase staining of serial sections of the heart of myocarditis for TCR ad chains and TCR V(10 confirmed that the dominant population of infiltrating T cells expressed VB10 gene products. Conclsuions The restricted usage of TCR genes by infiltrating T-cells may indicate that a specific antigen in heart with myocarditis is targeted. Our findings raise the possibility of immunotherapy with monoclonal antibodies specific for TCR Vie elements to prevent T-cell-mediated myocardial damage in viral myocarditis. (Circulton. 1994;89..2170-2175.)

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