In Situ Determination of Partition and Diffusion Coefficients in the Lipid Bilayers of Stratum Corneum

the requirement of isolating the SC lipid bilayers, and the In Situ Determination of Partition and requirement of fluorescently labeling drugs, which cannot be Diffusion Coefficients in the Lipid performed in many cases. Thus, there is a need for a simple method that can be: i) used to determine drug partition and Bilayers of Stratum Corneum diffusion coefficients in the SC without requiring the removal of the lipid bilayers from the SC, and ii) used to understand the dependence of enhancers on the SC transport properties. Samir Mitragotri1,2 In this paper, we describe such a method based on a combined theoretical and experimental approach. The method is based on two independent measurements of the transport propReceived January 14, 2000; accepted April 27, 2000 erties of the SC: i) steady-state permeability in the presence of