Radiation Damage Centers in Cholesteryl Heptanoate

Cholesterol takes part signi cantly in many biological mechanisms and as important component for manufacture of bile acids, steroid hormones, and several fat-soluble vitamins. To determine magnetic properties of cholesteryl heptanoate (C34H58O2) which is an important cholesteryl ester in human life and new technology, the single crystals of cholesteryl heptanoate were grown by slow evaporation of concentrated ethyl acetate solution and the grown single crystals were irradiated at room temperature with Co γ ray. The radical produced by gamma irradiation has been investigated in the range of temperatures 123 330 K for di erent orientations of the crystal in a magnetic eld by EPR. Radiation damage center was attributed to radical ĊHαCH2β . The g factor and hyper ne coupling constants have slight dependence on temperature and evident dependence on the orientation of the magnetic eld. Determined g factor and hyper ne coupling constants for the radical ĊHαCH2β were found to be anisotropic with the average values gav = 2.0036, (aCHα)av = 14.52 G, (aCH2β )av = 25.78 G.

[1]  H. Yüksel,et al.  Magnetic properties of gamma irradiated single crystals of cholesteryl chloride: An EPR study , 2011 .

[2]  Elina Ikonen,et al.  Cellular cholesterol trafficking and compartmentalization , 2008, Nature Reviews Molecular Cell Biology.

[3]  B. Aşik,et al.  EPR of gamma irradiated single crystals of cholesteryl benzoate , 2004 .

[4]  B. Yiǧit,et al.  ESR of gamma irradiated single crystals of cholesteryl acetate and cholesteryl chloroformate , 2003 .

[5]  A. Szyczewski.  EPR/ENDOR investigations of γ-irradiated steroid hormone single crystals , 1996 .

[6]  K. Möbius,et al.  An ENDOR study of the radicals in a γ-irradiated single crystal of 17α,21-dihydroxyprogesterone , 1994 .

[7]  R. Krzyminiewski,et al.  An ESR study of the stable radical in a γ-irradiated single crystal of 17α-dydroxy-progesterone , 1990 .

[8]  R. Krzyminiewski,et al.  Radiation damage in androst-4-en-3,17-dione molecules: an ESR study of free radicals in single crystals , 1987 .

[9]  M. Sevilla,et al.  An electron spin resonance investigation of radical intermediates in cholesterol and related compounds: relation to solid-state autoxidation , 1986 .

[10]  R. Krzyminiewski,et al.  An EPR study of γ-irradiated single crystals of cholesta-4, 6-diene-3-one , 1985 .

[11]  R. Krzyminiewski,et al.  EPR study of the stable radical in a γ-irradiated cholest-4-en-3-one single crystal , 1983 .

[12]  R. Krzyminiewski,et al.  EPR study of the stable radical in a γ-irradiated single crystal of progesterone , 1982 .

[13]  I. W. Levin,et al.  Cholesterol-lipid interactions: An infrared and raman spectroscopic study of the carbonyl stretching mode region of 1,2-dipalmitoyl phosphatidylcholine bilayers , 1980 .

[14]  L. J. Lis,et al.  Structural studies of cholesteryl acetate - phosphatidylcholine complexes , 1977 .

[15]  D. A. Long,et al.  A Raman spectroscopic study of the effect of hydrocarbon chain length and chain unsaturation on lecithin‐cholesterol interaction , 1976 .

[16]  H. N. Rexroad,et al.  ELECTRON-SPIN RESONANCE STUDIES OF RADIATION DAMAGE TO CERTAIN LIPIDS, HORMONES, AND VITAMINS. , 1959, Proceedings of the National Academy of Sciences of the United States of America.

[17]  B. Smaller,et al.  Paramagnetic Species Produced by γ Irradiation of Organic Compounds , 1958 .

[18]  B. Endeward,et al.  ENDOR study of γ-irradiated hydrated testosterone orthorhombic single crystals , 1998 .

[19]  R. Krzyminiewski,et al.  EPR study of hydrated testosterone monoclinic and orthorhombic single crystals gamma-irradiated at 295 K. , 1986, International journal of radiation biology and related studies in physics, chemistry, and medicine.