A novel biotinylated degradable polymer for cell-interactive applications.

We describe the development of a novel biodegradable polymer designed to present bioactive motifs at the surfaces of materials of any architecture. The polymer is a block copolymer of biotinylated poly(ethylene glycol) (PEG) with poly(lactic acid) (PLA); it utilizes the high-affinity coupling of the biotin-avidin system to undergo postfabrication surface engineering. We show, using surface plasmon resonance analysis (SPR) and confocal microscopy that surface engineering can be achieved under aqueous conditions in short time periods. These surfaces interact with cell surface molecules and generate beneficial responses as demonstrated by the model study of integrin-mediated spreading of endothelial cells on polymer surfaces presenting RGD peptide adhesion sequences.

[1]  E. Diamandis,et al.  The biotin-(strept)avidin system: principles and applications in biotechnology. , 1991, Clinical chemistry.

[2]  Robert Langer,et al.  Synthesis and RGD peptide modification of a new biodegradable copolymer: poly(lactic acid-co-lysine) , 1993 .

[3]  J. Hubbell,et al.  Solution technique to incorporate polyethylene oxide and other water-soluble polymers into surfaces of polymeric biomaterials. , 1991, Biomaterials.

[4]  Green Nm,et al.  Avidin and streptavidin. , 1990 .

[5]  Barry S. Coller,et al.  Immobilized Arg-Gly-Asp (RGD) peptides of varying lengths as structural probes of the platelet glycoprotein IIb/IIIa receptor. , 1992 .

[6]  E. Kretschmann.  Die Bestimmung optischer Konstanten von Metallen durch Anregung von Oberflächenplasmaschwingungen , 1971 .

[7]  J. Israelachvili,et al.  Direct Measurement of a Tethered Ligand-Receptor Interaction Potential , 1997, Science.

[8]  S. Shalaby,et al.  Biomedical polymers : designed-to-degrade systems , 1994 .

[9]  E Ruoslahti,et al.  Variants of the cell recognition site of fibronectin that retain attachment-promoting activity. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Erkki Ruoslahti,et al.  Organ targeting In vivo using phage display peptide libraries , 1996, Nature.

[11]  Jeffrey A. Hubbell,et al.  Lactide-based poly(ethylene glycol) polymer networks for scaffolds in tissue engineering. , 1996 .

[12]  M. Wilchek,et al.  Biotin-containing reagents. , 1990, Methods in enzymology.

[13]  D. Lauffenburger,et al.  Use of the Green Fluorescent Protein as a Quantitative Reporter of Epidermal Growth Factor Receptor-Mediated Gene Delivery , 1997 .

[14]  Anthony Atala,et al.  Synthetic Biodegradable Polymer Scaffolds , 1997, Birkhäuser Boston.

[15]  A. Hoffman.  Molecular bioengineering of biomaterials in the 1990s and beyond: a growing liaison of polymers with molecular biology. , 2008, Artificial organs.

[16]  Kenneth M. Yamada,et al.  Adhesive recognition sequences. , 1991, The Journal of biological chemistry.

[17]  Soriano,et al.  The Adsorption of Poly(vinyl alcohol) to Biodegradable Microparticles Studied by X-Ray Photoelectron Spectroscopy (XPS) , 1997, Journal of colloid and interface science.

[18]  J. Vacanti,et al.  Tissue engineering : Frontiers in biotechnology , 1993 .

[19]  Erkki Ruoslahti,et al.  Phage Libraries Displaying Cyclic Peptides with Different Ring Sizes: Ligand Specificities of the RGD-Directed Integrins , 1995, Bio/Technology.

[20]  R. Melton,et al.  Polyethylene glycol modification of a galactosylated streptavidin clearing agent: effects on immunogenicity and clearance of a biotinylated anti-tumour antibody. , 1996, British Journal of Cancer.

[21]  M C Davies,et al.  Surface plasmon resonance for real time in situ analysis of protein adsorption to polymer surfaces. , 1997, Biomaterials.

[22]  N A Peppas,et al.  New challenges in biomaterials. , 1994, Science.