Phytochemical Screening and Antimicrobial Activities of Siphonochilus aethiopicus Extracts from Benin

Siphonochilus aethiopicus (Schweinf.) B.L.Burtt (Zingiberaceae), a wild ginger used in traditional medicine in Benin, is investigated for the antibacterial and antifungal activities. Biological activities of ethyl acetate and ethanolic extracts are assessed against six bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, methicillin resistant Staphylococcus aureus and Staphylococcus epidermidis) and three fungi strains (Aspergillus clavatus, Aspergillus ochraceus and Aspergillus parasiticus). Minimal inhibitory concentration is determined by microdilution tests using iodonitrotetrazolium salt as bacterial growth indicator. Inhibition of sporulation and mycelia growth are performed using agar diffusion. Siphonochilus aethiopicus is screened for phytochemical constituents. Ethyl acetate extract of the dry rhizome of S. aethiopicus showed antibacterial activities against three strains: methicillin resistant Staphylococcus aureus, Staphylococcus aureus and Escherichia coli with a minimal inhibitory concentration of 5 mg/mL. Both extracts are effective on mycelia growth of the three fungi strains with an inhibitory percentage between 40.95±1.65 and 63.50±1.26. The same result is observed for sporulation range from 42.17±0.00 to 61.46±0.006. The phytochemicals alkaloids, flavonoids, triterpenes and steroids are, found in both extracts, and could be responsible of biological activities.

[1]  Michael Wink,et al.  Modes of Action of Herbal Medicines and Plant Secondary Metabolites , 2015, Medicines.

[2]  Rajbir Kaur,et al.  ALKALOIDS-IMPORTANT THERAPEUTIC SECONDARY METABOLITES OF PLANT ORIGIN , 2015 .

[3]  Isabel Gómez-Betancur,et al.  Traditional use of the genus Renealmia and Renealmia alpinia (Rottb.) Maas (Zingiberaceae)-a review in the treatment of snakebites. , 2014, Asian Pacific journal of tropical medicine.

[4]  Fathima,et al.  Phytochemical Study of Hybanthus enneaspermus (Linn.) F.Muell , 2014 .

[5]  Quantifying the bioactivity of plant extracts during screening and bioassay-guided fractionation , 2014 .

[6]  G. Fouche,et al.  Siphonochilus aethiopicus, a traditional remedy for the treatment of allergic asthma , 2013 .

[7]  N. Ruiz,et al.  Anti-proliferative activity of four aromatic plants of Benin , 2013 .

[8]  H. Mary,et al.  Phytochemical characterization and antimicrobial activity of Curcuma xanthorrhiza Roxb. , 2012 .

[9]  Carol Clements,et al.  Bioactive diterpenes and sesquiterpenes from the rhizomes of wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt). , 2011, African journal of traditional, complementary, and alternative medicines : AJTCAM.

[10]  Z. A. Obanu,et al.  The volatile components of wild ginger (Siphonochilus aethiopicus (Schweinf) B.l Burtt) , 2011 .

[11]  S. Sulaiman,et al.  Essential oils of Zingiber officinale var. rubrum Theilade and their antibacterial activities , 2011 .

[12]  R. Coopoosamy,et al.  Screening of Siphonochilus aetiopicus (Schweinf.) B. L. Burtt for antibacterial and antifungal properties , 2010 .

[13]  Mattheos A. G. Koffas,et al.  Biosynthesis and biotechnological production of flavanones: current state and perspectives , 2009, Applied Microbiology and Biotechnology.

[14]  M. Rai,et al.  Antifungal Activity of Essential Oils from Indian Medicinal Plants Against Human Pathogenic Aspergillus fumigatus and A. niger , 2008 .

[15]  M. Kandefer-Szerszeń,et al.  Terpenes: substances useful in human healthcare , 2007, Archivum Immunologiae et Therapiae Experimentalis.

[16]  A. Singh,et al.  Antifungal Activity of an Alkaloid Allosecurinine against Some Fungi , 2007, Mycobiology.

[17]  Andrew J Lamb,et al.  Antimicrobial activity of flavonoids , 2005, International Journal of Antimicrobial Agents.

[18]  A. Badoc,et al.  Activité antifongique d'extraits de Thymelaea lythroides sur trois champignons pathogènes du riz , 2004 .

[19]  I. Jantan,et al.  Antifungal Activity of the Essential Oils of Nine Zingiberaceae Species , 2003 .

[20]  P. Wessels,et al.  Furanoterpenoids from Siphonochilus aethiopicus. , 2002, Phytochemistry.

[21]  A. Jäger,et al.  The essential oil composition of the roots and rhizomes of Siphonochilus aethiopicus , 2002 .

[22]  M. E. Light,et al.  Investigation of the biological activities of Siphonochilus aethiopicus and the effect of seasonal senescence , 2002 .

[23]  D. Srinivasan,et al.  Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. , 2001, Journal of ethnopharmacology.

[24]  J. Eloff.  A Sensitive and Quick Microplate Method to Determine the Minimal Inhibitory Concentration of Plant Extracts for Bacteria , 1998, Planta medica.

[25]  J. Eloff.  Which extractant should be used for the screening and isolation of antimicrobial components from plants? , 1998, Journal of ethnopharmacology.