Peroxydisulfate/oxygen system-based electrochemiluminescent immunosensing of Hg2+ using Pt/Pd nanodendrites-thiosemicarbazide/norfloxacin as a signal enhancer.

Herein, we describe a competitive-type electrochemiluminescence (ECL) strategy for Hg2+ determination based on the peroxydisulfate/oxygen (S2O82-/O2) system that uses Pt/Pd nanodendrites (Pt/Pd NDs)-thiosemicarbazide/norfloxacin (TN)-covered gold nanoparticles (Pt/Pd-TNG50) as a signal enhancer. The Pt/Pd NDs, a dense array of Pt branches on a Pd core, possessed excellent catalytic properties to enhance ECL intensity by accelerating electron transfer. In addition, the binary intramolecular synergy of TN, which had cooperative interactions of powerful π-π stacking with a larger conjugated surface, could extremely enhance the ECL signal of the S2O82-/O2 system. Furthermore, we designed a competitive immunoassay method using a structured sensor where a monoclonal antibody (mAb) against Hg2+ exhibited high specificity and recognition of Hg2+, which greatly improved the specificity and sensitivity of the immunosensor. As a result, the proposed immunosensor gave Hg2+ detection with a low detection limit (16 pg mL-1) and displayed high sensitivity and stability. Importantly, this work not only, for the first time to our knowledge, utilized Pt/Pd NDs as promising ECL emitters for bioprobe construction but also opened an efficient way for the detection of Hg2+ in environmental monitoring.

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