The location of blood group antigen A on cultured rabbit kidney cells as revealed by ferritin-labelled antibody.

SUMMARY Fluorescein- and ferritin-labelled antibodies were used to locate blood group antigen A on the surfaces of cultured rabbit kidney cells (RK 13). Fluorescent anti-A revealed the antigen on some cells, but not on others. Controls indicated that specific staining could be obtained. Comparable observations were made with ferritin-labelled antibody, and the comparatively high resolution of this method allowed individual antigen molecules to be located. Measurements were made of the distances between the centres of the ferritin molecules and the cell surface, and the position of the blood group antigen relative to the visible components of the membrane is discussed in relation to this.

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