Dielectrophoresis: Iron Dificient Anemic Red Blood Cells for Artificial Kidney Purposes

The main objective of this research is to address certain medical problems faced by kidney renal disease patients. A sample of iron deficient blood was studied using dielectrophoresis to determine any differences between the properties of anemic red blood cells and normal red blood cells. The crossover frequencies show that anemic red blood cells and normal red blood cells offer different results due to their differing properties. The results for crossover frequencies successfully demonstrate the differences between the red blood cell types. Crossover frequencies for normal red blood cells is at 380kHz, while the frequency for iron deficient red blood cells is at 7MHz. These differences in particle properties were proven by the analysis of crossover frequency differences using MATLAB.

[1]  Azrul Azlan Hamzah,et al.  Implementing the concept of dielectrophoresis in glomerular filtration of human kidneys , 2016, 2016 IEEE International Conference on Semiconductor Electronics (ICSE).

[2]  Guillaume Mernier,et al.  Separation of platelets from other blood cells in continuous-flow by dielectrophoresis field-flow-fractionation. , 2011, Biomicrofluidics.

[3]  J. E. Duarte,et al.  Non-uniform electric field-induced yeast cell electrokinetic behavior , 2008, Ingeniería e Investigación.

[4]  Azrul Azlan Hamzah,et al.  Finite element modeling of dielectrophoretic microelectrodes based on a array and ratchet type , 2014, 2014 IEEE International Conference on Semiconductor Electronics (ICSE2014).

[5]  Azrul Azlan Hamzah,et al.  Dielectrophoretic characterization of array type microelectrodes , 2014, 2014 IEEE International Conference on Semiconductor Electronics (ICSE2014).

[6]  R. Pethig,et al.  Dielectrophoresis: A Review of Applications for Stem Cell Research , 2010, Journal of biomedicine & biotechnology.

[7]  B. Majlis,et al.  Determination of lateral and vertical dielectrophoresis forces using tapered microelectrode array , 2018 .

[8]  Nadine Aubry,et al.  Particle Separation Using Dielectrophoresis , 2003 .

[9]  Azrul Azlan Hamzah,et al.  A Tapered Aluminium Microelectrode Array for Improvement of Dielectrophoresis-Based Particle Manipulation , 2015, Sensors.

[10]  Ronald Pethig,et al.  Positive and negative dielectrophoretic collection of colloidal particles using interdigitated castellated microelectrodes , 1992 .

[11]  Wendong Zhang,et al.  Portable microsystem integrates multifunctional dielectrophoresis manipulations and a surface stress biosensor to detect red blood cells for hemolytic anemia , 2016, Scientific Reports.

[12]  Prashanta Dutta,et al.  Dielectrophoretic separation of bioparticles in microdevices: A review , 2014, Electrophoresis.

[13]  F F Becker,et al.  Separation of human breast cancer cells from blood by differential dielectric affinity. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[14]  B. Majlis,et al.  Negative charge dielectrophoresis by using different radius of electrodes for biological particles , 2017, 2017 IEEE Regional Symposium on Micro and Nanoelectronics (RSM).

[15]  Macdiarmid,et al.  Dielectrophoretic Cell Separation : Some Hints and Kinks , 2010 .

[16]  Ronald Pethig,et al.  The removal of human leukaemia cells from blood using interdigitated microelectrodes , 1994 .

[17]  Michael P Hughes,et al.  Fifty years of dielectrophoretic cell separation technology. , 2016, Biomicrofluidics.

[18]  Azrul Azlan Hamzah,et al.  Sputtered Encapsulation as Wafer Level Packaging for Isolatable MEMS Devices: A Technique Demonstrated on a Capacitive Accelerometer , 2008, Sensors.