Study of level and selectivity of liposomal form of photosensitiser hydroxyaluminium tetra-3-phenylthiophthalocyanine accumulation on transplantable mice tumor models at different ways of transplantation

Background . Current work is devoted to the study in vivo of concentration and selectivity of accumulation of infrared photosensitizer (PS) hydroxyaluminium tetra-3-phenylthiophthalocyanine in liposomal form in intramuscularly and subcutaneously transplanted mice tumor models in comparison to normal tissues. Objective: to study the level and selectivity of accumulation of hydroxyaluminium tetra-3-phenylthiophthalocyanine liposomal form on mice tumor models in order to optimize the transplantation approach and the starting of photodynamic treatment. Materials and methods . A range of transplantable mice tumors was used in the study: solid carcinoma Ehrlich (ELD) and solid sarcoma S-37, epidermoid Lewis lung carcinoma (LLC) and colon adenocarcinoma (AKATOL). For the assessment of concentration of PS in tissues was evaluated by fluorescence spectroscopy in vivo. carcinoma Ehrlich (ELD) accumulation of when transplanted intramuscularly. after administration concentration of PS in mg/kg, selectivity normal tissue : maximum concentration of PS in sarcoma S-37 observed with subcutaneous transplantation, achieving 5 h after administration the value of 5.4 mg/kg with selectivity of accumulation 4.3 : 1. Both LLC and AKATOL showed optimum results with intramuscular transplantation. Maximum concentration of PS in 5 h after administration, achieving 7.5 mg/kg with selectivity exceeding Concentration of photosensitizer in AKATOL 7 h post achieved 6.8 mg/kg with selectivity about recommend the method of listed tumors models transplantation for the studies of PS.