Immobilization of enzyme (DAAO) on hybrid nanoporous MCF, SBA-15, and MCM-41 materials

Hybrid nanoporous MCF, SBA-15 and MCM-41 materials were synthesized via hydrothermal treatment and functionalized with 3-aminopropyltriethoxysilane (APTES) via post-synthesis grafting and sequently activated by glutardialdehyde and then were used to immobilize D-amino acid oxidase (DAAO). The amino-functionalized materials were characterized by various techniques: XRD, IR and N2 adsorption-desorption (BET). From characterization results, it indicated that these materials still maintained their structure after functionalization. The data IR and TGA-DTA analysis demonstrated the incorpotation of amine functional groups on the surface of APTES-functionalized samples. The DAAO immobilized on functionalized materials exhibited higher catalytic activity and stability for conversion of cephalosporin C (CPC) compare to those of non-functionalized one. Further more, the catalytic activity as well as stability of enzyme decreased in order MCF > SBA-15 > MCM-41 with the decrease of their pore size.

[1]  L. Fischer,et al.  Induction of the d-Amino Acid Oxidase from Trigonopsis variabilis , 1996, Applied and environmental microbiology.

[2]  X. Zhao,et al.  Functionalization of SBA-15 with APTES and Characterization of Functionalized Materials , 2003 .

[3]  G. Luo,et al.  Effect of pore diameter and cross-linking method on the immobilization efficiency of Candida rugosa lipase in SBA-15. , 2010, Bioresource technology.

[4]  I. Díaz,et al.  Bottle-around-the-ship: A method to encapsulate enzymes in ordered mesoporous materials , 2010 .

[5]  Chunxi Hai,et al.  Adsorption heterogeneity of lysozyme over functionalized mesoporous silica: Effect of interfacial noncovalent interactions , 2008 .

[6]  R. Jasra,et al.  Studies on the activity and stability of immobilized α-amylase in ordered mesoporous silicas , 2005 .

[7]  S. Sugunan,et al.  Tuning mesoporous molecular sieve SBA-15 for the immobilization of α-amylase , 2010 .

[8]  D. Zhao,et al.  Hydrophobic mesoporous materials for immobilization of enzymes , 2009 .

[9]  G. Lu,et al.  Functionalized Mesoporous Silica with Very Large Pores for Cellulase Immobilization , 2010 .

[10]  J. S. Beck,et al.  Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism , 1992, Nature.

[11]  Kwong-Yu Chan,et al.  Enzyme immobilization on amino-functionalized mesostructured cellular foam surfaces, characterization and catalytic properties , 2005 .

[12]  V. Tishkov,et al.  D-amino acid oxidase: structure, catalytic mechanism, and practical application , 2005, Biochemistry (Moscow).

[13]  Isabel Díaz,et al.  A comparative study of periodic mesoporous organosilica and different hydrophobic mesoporous silicas for lipase immobilization , 2010 .

[14]  L. Pollegioni,et al.  Properties and applications of microbial D-amino acid oxidases: current state and perspectives , 2008, Applied Microbiology and Biotechnology.

[15]  K. Balkus,et al.  Enzyme immobilization in MCM-41 molecular sieve , 1996 .

[16]  S. J. Gregg,et al.  Adsorption Surface Area and Porosity , 1967 .

[17]  Fredrickson,et al.  Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom pores , 1998, Science.

[18]  Jordan T. Watson,et al.  Catalytic activity of mesoporous silicate-immobilized chloroperoxidase , 2002 .

[19]  C. Mou,et al.  Cytochrome c covalently immobilized on mesoporous silicas as a peroxidase: Orientation effect , 2010 .

[20]  G. Stucky,et al.  Microemulsion Templating of Siliceous Mesostructured Cellular Foams with Well-Defined Ultralarge Mesopores , 2000 .

[21]  Dongyuan Zhao,et al.  Hexagonal to Mesocellular Foam Phase Transition in Polymer-Templated Mesoporous Silicas , 2000 .

[22]  Jun Ge,et al.  Recent advances in nanostructured biocatalysts , 2009 .

[23]  B. K. Hodnett,et al.  The adsorption characteristics, activity and stability of trypsin onto mesoporous silicates , 2005 .

[24]  R. Jasra,et al.  Immobilization of alkaline serine endopeptidase from Bacillus licheniformis on SBA-15 and MCF by surface covalent binding , 2009 .

[25]  Lucia Gardossi,et al.  Understanding enzyme immobilisation. , 2009, Chemical Society reviews.

[26]  A. Salis,et al.  Physical and chemical adsorption of Mucor javanicus lipase on SBA-15 mesoporous silica. Synthesis, structural characterization, and activity performance. , 2005, Langmuir.

[27]  K. Diederichs,et al.  Yeast D-amino acid oxidase: structural basis of its catalytic properties. , 2002, Journal of molecular biology.