论文信息 - A New Method For The Controlled Immobilization Of Enzyme In Inorganic Gels (laponite) For Amperometric Glucose Biosensor

A New Method For The Controlled Immobilization Of Enzyme In Inorganic Gels (laponite) For Amperometric Glucose Biosensor

The feasibility of an amperometric glucose biosensor based on immobilization of glucose oxidase (GOD) in inorganic gels was investigated. The gels modified with enzyme were deposited onto platinum electrodes and then were exposed to glutaraldehyde vapor in order to improve the performances of the biosensor. The calibration graph was linear from 0.1 to 4mM of glucose. The main advantage of this kind of biosensor is its long stability. The biosensor was kept for several weeks in a buffer solution at 4/spl deg/C. About 450 measurements were done without any loss of the linearity of the dynamic range (0.1mM-4mM).

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