The metallic nanoparticles have great attention of Chemists, Physists, Biologists and Engineers who wish to use them for development of new generation nanodevices. In this present study silver nanoparticles were synthesized from aqueous silver nitrate (1mM) through a simple and ecofriendly route using leaf broth of Ocimum sanctum as reductant and stabilizer. The aqueous silver ions when exposed to leaf broth were reduced and resulted in the green synthesis of silver nanoparticles ranges from 3-20 nm. The bioreduced silver nanoparticles were characterized by UVVis spectrophotometer, X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Fourier transform infra-red (FTIR) spectroscopy. The observed peaks in XRD pattern corresponding to (111), (200) and (311) planes. The FTIR measurement was carried out to identify the possible biomolecules responsible for efficient stabilization of silver nanoparticles