Rapid Micropropagation and Callus Induction of Catharanthus roseus in Vitro Using Different Explants

3 Abstract: The effect of plant growth regulators on callus induction using different explants, to regenerate shoot/root from different explants as well as from calli in cultures of Catharanthus roseus (L.) G. Don was studied. Total of nineteen shoot regeneration media were used, out of these nineteen media three were further used to see the regeneration response and for root regeneration four media were tested supplemented with different concentration of IBA. Based on the results of this study, earliest and maximum (99%) callus induction response was observed on 10 day of inoculation from hypocotyl explants under dark conditions on MS th