DNA FRAGMENTATION IN MICROORGANISMS ASSESSED IN SITU 2 3 4 Running title : DNA fragmentation in microorganisms 5 6 7

a INIBIC-Genética, Complejo Hospitalario Universitario Juan Canalejo, As Xubias 84, 11 15006A Coruña, Spain. 12 13 b Laboratorio de Genética Molecular y Radiobiología, Centro Oncológico de Galicia, 14 Avda. de Montserrat s/n, 15009-A Coruña, Spain. 15 16 c Unidad de Genética, Facultad de Biología, Universidad Autónoma de Madrid, 2804917 Madrid, Spain. 18 19 d INIBIC-Microbiología, Complejo Hospitalario Universitario Juan Canalejo, As 20 Xubias, 84, 15006-A Coruña, Spain 21 22

[1]  P. de Paz,et al.  DNA fragmentation assessment by flow cytometry and Sperm-Bos-Halomax (bright-field microscopy and fluorescence microscopy) in bull sperm. , 2007, International journal of andrology.

[2]  K. Drlica,et al.  Effect of Anaerobic Growth on Quinolone Lethality with Escherichia coli , 2006, Antimicrobial Agents and Chemotherapy.

[3]  Xilin Zhao,et al.  Lethal fragmentation of bacterial chromosomes mediated by DNA gyrase and quinolones , 2006, Molecular microbiology.

[4]  J. Gosálvez,et al.  A new method to analyze boar sperm DNA fragmentation under bright-field or fluorescence microscopy. , 2006, Theriogenology.

[5]  J. Czub,et al.  Interaction of amphotericin B and its selected derivatives with membranes: molecular modeling studies. , 2006, Chemical record.

[6]  P. Olive,et al.  Heterogeneity in DNA damage using the comet assay , 2005, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[7]  A. Aertsen,et al.  Stress and How Bacteria Cope with Death and Survival , 2004, Critical reviews in microbiology.

[8]  E. Herker,et al.  Apoptosis in yeast: a new model system with applications in cell biology and medicine , 2002, Current Genetics.

[9]  J. Gosálvez,et al.  DNA Breakage Detection-FISH (DBD-FISH) , 2002 .

[10]  D. Hooper,et al.  Mechanisms of action of antimicrobials: focus on fluoroquinolones. , 2001, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[11]  K. Lewis,et al.  Programmed Death in Bacteria , 2000, Microbiology and Molecular Biology Reviews.

[12]  K. Bayles The bactericidal action of penicillin: new clues to an unsolved mystery. , 2000, Trends in microbiology.

[13]  S. Nagata Apoptotic DNA fragmentation. , 2000, Experimental cell research.

[14]  N. Singh,et al.  A simple method for accurate estimation of apoptotic cells. , 2000, Experimental cell research.

[15]  F. Rohwer,et al.  Detection of DNA Damage in Prokaryotes by Terminal Deoxyribonucleotide Transferase-Mediated dUTP Nick End Labeling , 2000, Applied and Environmental Microbiology.

[16]  D. Dubnau,et al.  DNA uptake in bacteria. , 1999, Annual review of microbiology.

[17]  N. Singh,et al.  Visual quantification of DNA double-strand breaks in bacteria. , 1999, Mutation research.

[18]  P. Lebaron,et al.  Comparison of Blue Nucleic Acid Dyes for Flow Cytometric Enumeration of Bacteria in Aquatic Systems , 1998, Applied and Environmental Microbiology.

[19]  M. Yarmolinsky,et al.  Programmed cell death in bacterial populations , 1995, Science.

[20]  G. Ahnström Techniques to measure DNA single-strand breaks in cells: a review. , 1988, International journal of radiation biology.

[21]  Arlen W. Johnson,et al.  Exonuclease III and endonuclease IV remove 3' blocks from DNA synthesis primers in H2O2-damaged Escherichia coli. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[22]  M. Herrero,et al.  Microcin B17 blocks DNA replication and induces the SOS system in Escherichia coli. , 1986, Journal of general microbiology.

[23]  J. Ward Radiation-induced strand breakage in DNA. , 1975, Basic life sciences.

[24]  K. Arima,et al.  Properties of the colicin E 2 -induced degradation of deoxyribonucleic acid in Escherichia coli. , 1971, Journal of biochemistry.