SEXUAL DIFFERENCES IN SEROTONIN DISTRIBUTION AND INDUCTION OF SYNCHRONOUS LARVAL RELEASE BY SEROTONIN IN THE FRESHWATER MUSSEL HYRIOPSIS BIALATUS

We have established an in vitro glochidial culture of the freshwater mussel, Hyriopsis (Hyriopsis) bialatus, with the potential of restoring natural populations. However, to develop a successful in vitro culture process the synchronous release of glochidia needs to be achieved. In this study we investigated the distribution of serotonin and its effect on inducing synchronous parturition. Using confocal microscopy on whole-mounted tissues, we show that serotonin is highly expressed in the peripheral neuron somata and their axonal processes in the central neuropils of the visceral ganglion. Serotonergic nerve fibres were also detected along the branchial nerve innervating the demibranchs. A hierarchical ramification from the branchial cord into nerve rootlets and subsequently into the small nerve fibres within the demibranch epithelium was evident throughout this organ. In both visceral ganglia and demibranchs, immunoreactivity of serotonin was relatively higher in female than male tissues, suggesting a role of serotonin in female reproductive functions. Administration of serotonin to the demibranchs, the sites of larval settlement, stimulated synchronous parturition in a dosedependent manner. A single-dose injection of serotonin yielded a large number of released larvae within 1–2 h, with no adverse effect on the quality of cultured larvae.

[1]  P. Fong,et al.  SEROTONIN-INDUCED PARTURITION IN THE FINGERNAIL CLAM SPHAERIUM (MUSCULIUM)TRANSVERSUM (SAY) , 1995 .

[2]  A. Eble Chapter 4 Anatomy and histology of Mercenaria mercenaria , 2001 .

[3]  K. Vining,et al.  An endogenous SCP-related peptide modulates ciliary beating in the gills of a venerid clam, Mercenaria mercenaria. , 1999, The Biological bulletin.

[4]  J. Hardege,et al.  In vivo and in vitro induction of germinal vesicle breakdown in a freshwater bivalve, the zebra mussel Dreissena polymorpha (Pallas). , 1994, The Journal of experimental zoology.

[5]  Louis F Gainey,et al.  Branchial Musculature of a Venerid Clam: Pharmacology, Distribution, and Innervation , 2003, The Biological Bulletin.

[6]  Margaret A Carroll,et al.  The nervous system control of lateral ciliary activity of the gill of the bivalve mollusc, Crassostrea virginica. , 2007, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[7]  K. Kyozuka,et al.  Serotonergic Ligands Induce Spawning but not Oocyte Maturation in the Bivalve Mactra chinensis from Central Japan. , 1996, The Biological bulletin.

[8]  S. Kovitvadhi,et al.  Digestive enzymes and in-vitro digestibility of different species of phytoplankton for culture of the freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus , 2008, Aquaculture International.

[9]  S. Medler,et al.  Functional Organization of Intrinsic Gill Muscles in Zebra Mussels, Dreissena polymorpha (Mollusca: Bivalvia), and Response to Transmitters in Vitro , 1997 .

[10]  E. Catapane The peripheral innervation of the gill of the marine mollusc demonstrated by the aluminium-formaldehyde (ALFA) histofluorescence method , 2004, Cell and Tissue Research.

[11]  K. Kuwasawa,et al.  Neuronal and neurohormonal control of the heart in the stomatopod crustacean, Squilla oratoria , 2004, Journal of Experimental Biology.

[12]  L. Vilarinho,et al.  Study of a suitable fish plasma for in vitro culture of glochidia Hyriopsis myersiana , 2002 .

[13]  W. Weerachatyanukul,et al.  Localization of serotonin in neuronal ganglia of the freshwater pearl mussel, Hyriopsis (Hyriopsis) bialata , 2010 .

[14]  G. Stefano Neurobiology of Mytilus edulis , 1990 .

[15]  B. Hochner,et al.  Serotonin is a facilitatory neuromodulator of synaptic transmission and “reinforces” long-term potentiation induction in the vertical lobe of Octopus vulgaris , 2010, Neuroscience.

[16]  T. Matsutani,et al.  Localization of monoamines in the central nervous system and gonad of the scallop Patinopecten yessoensis. , 1984 .

[17]  H. Kobayashi,et al.  Aminergic cellular organization in the gills of Aplysia species. , 1989, Journal of neurobiology.

[18]  J. Kraeuter,et al.  Biology of the hard clam , 2001 .

[19]  R. McMahon,et al.  11 – MOLLUSCA: BIVALVIA , 2001 .

[20]  J. Machado,et al.  Parturition in Anodonta cygnea induced by selective serotonin reuptake inhibitors (SSRIs) , 2001 .

[21]  T. Lee,et al.  SCP-Related Peptides From Bivalve Mollusks: Identification, Tissue Distribution, and Actions. , 1993, The Biological bulletin.

[22]  Takeo Kishimoto,et al.  Induction of spawning and oocyte maturation by 5-hydroxytryptamine in the surf clam , 1988 .

[23]  C. Blaise,et al.  Immunohistochemical localization of serotonin (5-hydroxytryptamine) in the gonad and digestive gland of Mya arenaria (Mollusca: Bivalvia). , 2006, General and comparative endocrinology.

[24]  K. Kyozuka,et al.  Spawning in the zebra mussel (Dreissena polymorpha): activation by internal or external application of serotonin. , 1993, The Journal of experimental zoology.

[25]  H Silverman,et al.  Musculature Associated with the Water Canals in Freshwater Mussels and Response to Monoamines In Vitro. , 1991, The Biological bulletin.

[26]  D. Sonetti,et al.  Serotonergic neurotransmission in the bivalve Venus verrucosa (Veneridae): a neurochemical and immunohistochemical study of the visceral ganglion and gonads , 2004 .

[27]  J. Machado,et al.  Culture of glochidia of the freshwater pearl mussel Hyriopsis myersiana (Lea, 1856) in artificial media , 2001 .

[28]  T. Roeder,et al.  Octopamine in invertebrates , 1999, Progress in Neurobiology.

[29]  S. Kovitvadhi,et al.  Optimization of diet and culture environment for larvae and juvenile freshwater pearl mussels, Hyriopsis (Limnoscapha) myersiana Lea, 1856 , 2006 .

[30]  R. Deguchi,et al.  Serotonin-induced meiosis reinitiation from the first prophase and from the first metaphase in oocytes of the marine bivalve Hiatella flaccida: respective changes in intracellular Ca2+ and pH. , 1995, Developmental biology.