Detection of Severe Acute Respiratory Syndrome Coronavirus in Blood of Infected Patients

ABSTRACT Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.

[1]  J. Temple,et al.  Frequent Recovery and Broad Genotype 2 Diversity Characterize Hepatitis C Virus Infection in Ghana, West Africa , 2003, Journal of Virology.

[2]  Y. Guan,et al.  Rapid Diagnosis of a Coronavirus Associated with Severe Acute Respiratory Syndrome (SARS) , 2003, Clinical chemistry.

[3]  Obi L. Griffith,et al.  The Genome Sequence of the SARS-Associated Coronavirus , 2003, Science.

[4]  Philip M. Long,et al.  Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection , 2003, The Lancet.

[5]  L. Poon,et al.  Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia : a prospective study , 2003 .

[6]  Christian Drosten,et al.  Identification of a novel coronavirus in patients with severe acute respiratory syndrome. , 2003, The New England journal of medicine.

[7]  J. A. Comer,et al.  A novel coronavirus associated with severe acute respiratory syndrome. , 2003, The New England journal of medicine.

[8]  Y. Guan,et al.  Coronavirus as a possible cause of severe acute respiratory syndrome , 2003, The Lancet.

[9]  M. Mccarthy.  US military prepares for Iraq to use chemical and biological weapons , 2003, The Lancet.

[10]  L. F. Ng,et al.  Membrane Association and Dimerization of a Cysteine-Rich, 16-Kilodalton Polypeptide Released from the C-Terminal Region of the Coronavirus Infectious Bronchitis Virus 1a Polyprotein , 2002, Journal of Virology.

[11]  D. Cavanagh.  Nidovirales: a new order comprising Coronaviridae and Arteriviridae. , 1997, Archives of virology.