Silicon microfabricated device for a non-sheath-flow cytometer
暂无分享,去创建一个
We report on the design and fabrication of a low Reynolds number silicon/pyrex microfluidic device that forces particles flowing in a microchannel to a tightly-focused equilibrium position. This device can be used for optical biomedical systems such as a flow cytometer so that particles can flow in a precise position without sheath flow for precision scattering or fluorescence measurements. Devices with a range of dimensions were fabricated using photolithography and wet chemical etching techniques. Particle positions in the channel are determined by observing the direct image as well as two reflection images from the sloping, polished channel walls. We tested devices with various ratios of width to particle diameter at a range of flow rates. When scattering signals from 10 micrometers polymer beads are measured, we observe that the tight equilibrium position attained by the particles results in a much smaller standard deviation of the scattering signal than is observed in other microflow channel devices that do not focus the particles to equilibrium positions.
[1] G. Segré,et al. Behaviour of macroscopic rigid spheres in Poiseuille flow Part 2. Experimental results and interpretation , 1962, Journal of Fluid Mechanics.
[2] Paul Yager,et al. Development of flow-cytometry-based miniature chemical fluid analysis system using fluorescent microbeads , 1998, Photonics West - Biomedical Optics.
[3] L. G. Leal,et al. Inertial migration of rigid spheres in two-dimensional unidirectional flows , 1974, Journal of Fluid Mechanics.
[4] G. Wallis,et al. Field Assisted Glass‐Metal Sealing , 1969 .