3D Printing of Cytocompatible Water-Based Light-Cured Polyurethane with Hyaluronic Acid for Cartilage Tissue Engineering Applications

Diseases in articular cartilages have affected millions of people globally. Although the biochemical and cellular composition of articular cartilages is relatively simple, there is a limitation in the self-repair ability of the cartilage. Therefore, developing strategies for cartilage repair is very important. Here, we report on a new liquid resin preparation process of water-based polyurethane based photosensitive materials with hyaluronic acid with application of the materials for 3D printed customized cartilage scaffolds. The scaffold has high cytocompatibility and is one that closely mimics the mechanical properties of articular cartilages. It is suitable for culturing human Wharton’s jelly mesenchymal stem cells (hWJMSCs) and the cells in this case showed an excellent chondrogenic differentiation capacity. We consider that the 3D printing hybrid scaffolds may have potential in customized tissue engineering and also facilitate the development of cartilage tissue engineering.

[1]  R. Reiter,et al.  Stage-related capacity for limb chondrogenesis in cell culture. , 1977, Developmental biology.

[2]  A. Gobbi,et al.  One-Stage Cartilage Repair Using a Hyaluronic Acid–Based Scaffold With Activated Bone Marrow–Derived Mesenchymal Stem Cells Compared With Microfracture , 2016, The American journal of sports medicine.

[3]  H. Stanley,et al.  Raman spectroscopy: a structural probe of glycosaminoglycans. , 1978, Biochimica et biophysica acta.

[4]  Linbo Wu,et al.  In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells , 2005, Cell and Tissue Research.

[5]  P. R. van Weeren,et al.  Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs. , 2013, Macromolecular bioscience.

[6]  Jack G. Zhou,et al.  Current status of 4D printing technology and the potential of light-reactive smart materials as 4D printable materials , 2016 .

[7]  Jan Feijen,et al.  Designed biodegradable hydrogel structures prepared by stereolithography using poly(ethylene glycol)/poly(D,L-lactide)-based resins. , 2010, Journal of controlled release : official journal of the Controlled Release Society.

[8]  Shuying Yang,et al.  Hydrophilic polyurethane matrix promotes chondrogenesis of mesenchymal stem cells. , 2015, Materials science & engineering. C, Materials for biological applications.

[9]  R. Adhikari,et al.  Biodegradable synthetic polymers for tissue engineering. , 2003, European cells & materials.

[10]  Ling Qin,et al.  Cartilage regeneration using mesenchymal stem cells and a PLGA-gelatin/chondroitin/hyaluronate hybrid scaffold. , 2006, Biomaterials.

[11]  J. Rubin,et al.  Controlled gelation and degradation rates of injectable hyaluronic acid‐based hydrogels through a double crosslinking strategy , 2011, Journal of tissue engineering and regenerative medicine.

[12]  C. Kao,et al.  Laser Sintered Magnesium-Calcium Silicate/Poly-ε-Caprolactone Scaffold for Bone Tissue Engineering , 2017, Materials.

[13]  G. Khang,et al.  Poly (l-lactide-co-caprolactone) scaffolds enhanced with poly (β-hydroxybutyrate-co-β-hydroxyvalerate) microspheres for cartilage regeneration , 2013, Biomedical materials.

[14]  Chee Kai Chua,et al.  Porous polycaprolactone scaffold for cardiac tissue engineering fabricated by selective laser sintering. , 2010, Acta biomaterialia.

[15]  P. Gatenholm,et al.  3D Bioprinting Human Chondrocytes with Nanocellulose-Alginate Bioink for Cartilage Tissue Engineering Applications. , 2015, Biomacromolecules.

[16]  Daniel A Grande,et al.  Articular Cartilage Repair , 2013, Cartilage.

[17]  Ali Khademhosseini,et al.  Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography. , 2012, Biomaterials.

[18]  M. Alini,et al.  Degradable polymeric materials for osteosynthesis: tutorial. , 2008, European cells & materials.

[19]  Harri Korhonen,et al.  Preparation of poly(ε-caprolactone)-based tissue engineering scaffolds by stereolithography. , 2011, Acta biomaterialia.

[20]  Tae Gwan Park,et al.  Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering. , 2005, Biomaterials.

[21]  Peter Jüni,et al.  Viscosupplementation for Osteoarthritis of the Knee , 2012, Annals of Internal Medicine.

[22]  David Dean,et al.  Continuous digital light processing (cDLP): Highly accurate additive manufacturing of tissue engineered bone scaffolds , 2012, Virtual and physical prototyping.

[23]  Ryan B. Wicker,et al.  Fabrication of 3D Biocompatible/Biodegradable Micro-Scaffolds Using Dynamic Mask Projection Microstereolithography , 2009 .

[24]  C. V. van Blitterswijk,et al.  Critical factors in the design of growth factor releasing scaffolds for cartilage tissue engineering. , 2008, Expert Opinion on Drug Delivery.

[25]  Scott C. Brown,et al.  A three-dimensional osteochondral composite scaffold for articular cartilage repair. , 2002, Biomaterials.

[26]  R. Reis,et al.  Bilayered chitosan-based scaffolds for osteochondral tissue engineering: influence of hydroxyapatite on in vitro cytotoxicity and dynamic bioactivity studies in a specific double-chamber bioreactor. , 2009, Acta biomaterialia.

[27]  Jason A Burdick,et al.  Hydrogel design for cartilage tissue engineering: a case study with hyaluronic acid. , 2011, Biomaterials.

[28]  A I Caplan,et al.  Hyaluronic acid bonded to cell-culture surfaces stimulates chondrogenesis in stage 24 limb mesenchyme cell cultures. , 1986, Developmental biology.

[29]  Jiehua Li,et al.  Synthesis and micellization of new biodegradable phosphorylcholine-capped polyurethane , 2011 .

[30]  Jan Feijen,et al.  A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography. , 2009, Biomaterials.

[31]  Michael Szycher,et al.  Szycher's Handbook of Polyurethanes , 2012 .

[32]  Pei-Chen Su,et al.  Curing characteristics of shape memory polymers in 3D projection and laser stereolithography , 2017 .

[33]  Jens Petter Wold,et al.  Raman Spectra of Biological Samples: A Study of Preprocessing Methods , 2006, Applied spectroscopy.

[34]  P. Gikas,et al.  Current strategies for knee cartilage repair , 2010, International journal of clinical practice.

[35]  Shlomo Magdassi,et al.  4D printing shape memory polymers for dynamic jewellery and fashionwear , 2016 .

[36]  James J. Yoo,et al.  Hybrid printing of mechanically and biologically improved constructs for cartilage tissue engineering applications , 2012, Biofabrication.

[37]  S. Hsu,et al.  Enhanced migration of Wharton's jelly mesenchymal stem cells grown on polyurethane nanocomposites , 2013 .

[38]  Guangdong Zhou,et al.  Regeneration of a goat femoral head using a tissue-specific, biphasic scaffold fabricated with CAD/CAM technology. , 2013, Biomaterials.

[39]  Benjamin M Wu,et al.  Recent advances in 3D printing of biomaterials , 2015, Journal of Biological Engineering.

[40]  C. Laurencin,et al.  Biodegradable polymers as biomaterials , 2007 .

[41]  Ramille N Shah,et al.  Three-dimensional printing of soy protein scaffolds for tissue regeneration. , 2013, Tissue engineering. Part C, Methods.

[42]  S. Hsu,et al.  Synthesis and 3D Printing of Biodegradable Polyurethane Elastomer by a Water‐Based Process for Cartilage Tissue Engineering Applications , 2014, Advanced healthcare materials.

[43]  Maurilio Marcacci,et al.  Scaffold-based repair for cartilage healing: a systematic review and technical note. , 2013, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[44]  B. Heng,et al.  Functional biomaterials for cartilage regeneration. , 2012, Journal of biomedical materials research. Part A.

[45]  Yujiang Fan,et al.  Collagen hydrogel as an immunomodulatory scaffold in cartilage tissue engineering. , 2014, Journal of biomedical materials research. Part B, Applied biomaterials.

[46]  A. Albertsson,et al.  In vitro and in vivo degradation profile of aliphatic polyesters subjected to electron beam sterilization. , 2011, Acta biomaterialia.

[47]  Dongan Wang,et al.  Creating a Living Hyaline Cartilage Graft Free from Non‐Cartilaginous Constituents: An Intermediate Role of a Biomaterial Scaffold , 2012 .

[48]  Yuquan Wei,et al.  In vivo biocompatibility and osteogenesis of electrospun poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone)/nano-hydroxyapatite composite scaffold. , 2012, Biomaterials.