论文信息 - Design and development of immunoassays for detection of proteins

Design and development of immunoassays for detection of proteins

Abstract Immunoassay methodology is currently the method of choice for the quantitative and semi-quantitative detection of many types of proteins in complex mixtures. A combination of sensitivity, specificity and cost-effectiveness in terms of analytical performance are allied to a diverse array of assay formats suitable for laboratory and field use. In the present article, the problems of setting up immunoassays for novel food proteins, and the questions that need to be answered if a successful outcome is to be achieved, are discussed. Speculation on future developments in immunochemistry leads to the conclusion that antibody technology will play an important role in detection of novel proteins from genetically modified organisms.

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