Recombinant interleukin‐1α inhibits the growth of rat mesangial cells in culture

We have investigated the effect of interleukin‐1 (IL‐I) on the growth of mesangial cells isolated from rat kidney. Recombinant IL‐1α inhibited 3H‐thymidine uptake by mesangial cells in a dose‐dependent manner in the presence of either 0.5% or 5% fetal bovine serum (FBS). In the presence of high concentration of FBS(10%), the effect of IL‐1 was not prominent. The inhibitory effect of IL‐1 on the growth of mesangial cells was also confirmed by a change in cell numbers and measurements of protein synthesis with 3H‐leucine. IL‐1‐induced inhibition of mesangial cell growth was not affected by indomethacin. IL‐1 showed no effects on intracellular Ca2+ levels in mesangial cells. These observations indicate that IL‐1 inhibits the growth of mesangial cells, and thus may play a protective role for mesangial cells from abnormal proliferation in some pathophysiological states.

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