In vitro toxicity of MTA compared with other primary teeth pulpotomy agents.

OBJECTIVE The main goal of this work is to compare the In vitro toxicity of MTA with other primary teeth pulpotomy agents. STUDY DESIGN The In vitro toxicity of MTA, calcium hydroxide, ferric sulphate solution, diluted formocresol and Buckley's formocresol were tested using MTT and Neutral Red Uptake cell viability assays. The results for MTA were compared to those obtained for the other substances using ANOVA and Tukey statistical tests (p < 0.05). RESULTS MTA had the lower in vitro toxicity and Buckley's formocresol, the higher, with statically significant difference. CONCLUSION Among the primary teeth pulpotomy agents tested, MTA showed the lower In vitro toxicity, standing as the most promising substitute to formocresol.

[1]  G. Schmalz.  Root Canal Filling Materials , 2009 .

[2]  M. L. Hunter,et al.  Vital pulpotomy in the primary dentition: attitudes and practices of community dental staff in Wales. , 2007, International journal of paediatric dentistry.

[3]  S. Dadvand,et al.  Randomized controlled trial of mineral trioxide aggregate and formocresol for pulpotomy in primary molar teeth. , 2007, International endodontic journal.

[4]  L. Ye,et al.  Evaluation of the formocresol versus mineral trioxide aggregate primary molar pulpotomy: a meta-analysis. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[5]  P. Abbott,et al.  Clinical, radiographic and histological analysis of the effects of mineral trioxide aggregate used in direct pulp capping and pulpotomies of primary teeth. , 2006, Australian dental journal.

[6]  Suwanna Korsuwannawong,et al.  Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[7]  G. N. da Silva,et al.  Genotoxicity in primary human peripheral lymphocytes after exposure to regular and white mineral trioxide aggregate. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[8]  E. Bodrumlu,et al.  Reactions of connective tissue to amalgam, intermediate restorative material, mineral trioxide aggregate, and mineral trioxide aggregate mixed with chlorhexidine. , 2006, Journal of endodontics.

[9]  S. Koka,et al.  Cellular toxicity of mineral trioxide aggregate mixed with an alternative delivery vehicle. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[10]  J. Camilleri,et al.  Mineral trioxide aggregate: a review of the constituents and biological properties of the material. , 2006, International endodontic journal.

[11]  M. Qudeimat,et al.  A prospective clinical study of mineral trioxide aggregate for partial pulpotomy in cariously exposed permanent teeth. , 2006, Journal of endodontics.

[12]  A. Milnes.  Persuasive evidence that formocresol use in pediatric dentistry is safe. , 2006, Journal.

[13]  C. Bincoletto,et al.  Cytotoxicity of materials used in perforation repair tested using the V79 fibroblast cell line and the granulocyte-macrophage progenitor cells. , 2006, International endodontic journal.

[14]  D. Salvadori,et al.  Ex vivo biocompatibility tests of regular and white forms of mineral trioxide aggregate. , 2006, International endodontic journal.

[15]  M. Duggal,et al.  Mineral trioxide aggregate in primary molar pulpotomies , 2006, Evidence-Based Dentistry.

[16]  A. Crispin,et al.  Effectiveness of 4 Pulpotomy Techniques—Randomized Controlled Trial , 2005, Journal of dental research.

[17]  P. Judd,et al.  Do we still need formocresol in pediatric dentistry? , 2005, Journal.

[18]  A. Chuang,et al.  An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts. , 2005, Journal of endodontics.

[19]  B. Karabucak,et al.  Vital pulp therapy with mineral trioxide aggregate. , 2005, Dental traumatology : official publication of International Association for Dental Traumatology.

[20]  G. Holan,et al.  Long-term evaluation of pulpotomy in primary molars using mineral trioxide aggregate or formocresol. , 2005, Pediatric dentistry.

[21]  J. Gutmann,et al.  Histological and scanning electron microscopy assessment of various vital pulp-therapy materials. , 2003, Journal of endodontics.

[22]  R. Love,et al.  Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 2. Root-canal-filling materials. , 2003, International endodontic journal.

[23]  Emma-Christin Lönnroth,et al.  Cytotoxicity of liquids and powders of chemically different dental materials evaluated using dimethylthiazol diphenyltetrazolium and neutral red tests , 2003, Acta odontologica Scandinavica.

[24]  D. Carnes,et al.  Cytotoxicity of intracanal bleaching agents on periodontal ligament cells in vitro. , 2001, Journal of endodontics.

[25]  Q. Al-Jame,et al.  Ferric sulfate as pulpotomy agent in primary teeth: twenty month clinical follow-up. , 2000, The Journal of clinical pediatric dentistry.

[26]  G. Sletten,et al.  Cytotoxic effects of extracts of compomers. , 1999, Acta odontologica Scandinavica.

[27]  G. Leyhausen,et al.  Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. , 1998, Journal of biomedical materials research.

[28]  F. Vertucci,et al.  Cytotoxicity of endodontic materials. , 1998, Journal of endodontics.

[29]  P. Cleaton-jones,et al.  Pulp response to ferric sulfate, diluted formocresol and IRM in pulpotomized primary baboon teeth. , 1997, ASDC journal of dentistry for children.

[30]  M. Carreras,et al.  Pulpal tissue reaction to formocresol vs. ferric sulfate in pulpotomized rat teeth. , 1997, The Journal of clinical pediatric dentistry.

[31]  G. Schmalz,et al.  Toxicity parameters for cytotoxicity testing of dental materials in two different mammalian cell lines. , 1996, European journal of oral sciences.

[32]  J. Wataha,et al.  In vitro models of biocompatibility: a review. , 1996, Dental materials : official publication of the Academy of Dental Materials.

[33]  J. Wataha,et al.  Cytotoxic Interactive Effects of Dentin Bonding Components on Mouse Fibroblasts , 1995, Journal of dental research.

[34]  J. Wataha,et al.  Cytotoxicity and Dentin Permeability of Carbamide Peroxide and Hydrogen Peroxide Vital Bleaching Materials, in vitro , 1993, Journal of dental research.

[35]  Andrew O. Martinez,et al.  A rapid and simple MTT-based spectrophotometric assay for determining drug sensitivity in monolayer cultures , 1988 .

[36]  W. Seow.  Modulation of polymorphonuclear leukocyte adherence by pulpotomy medicaments: effects of formocresol, glutaraldehyde, eugenol, and calcium hydroxide , 1986 .

[37]  E Borenfreund,et al.  Toxicity determined in vitro by morphological alterations and neutral red absorption. , 1985, Toxicology letters.