Premarket Evaluation of a Commercial Glycoprotein G-Based Enzyme Immunoassay for Herpes Simplex Virus Type-Specific Antibodies

ABSTRACT A new commercial glycoprotein G-based enzyme immunoassay (gG-EIA) was compared with Western blotting (WB) for detection of herpes simplex virus type 1 (HSV-1) or HSV-2 type-specific antibodies in 193 serum samples. Sensitivity for HSV-1 was 95%; specificity was 96%. Sensitivity for HSV-2 was 98%; specificity was 97%. Twelve of 13 serum samples with equivocal gG-EIA results were serotyped by WB.

[1]  J. Zeh,et al.  Suppression of Subclinical Shedding of Herpes Simplex Virus Type 2 with Acyclovir , 1996, Annals of Internal Medicine.

[2]  L. Magder,et al.  A seroepidemiologic survey of the prevalence of herpes simplex virus type 2 infection in the United States. , 1989, The New England journal of medicine.

[3]  P. Griffiths,et al.  Clinical Management of Herpes Viruses , 1995 .

[4]  L. Corey,et al.  Inability of enzyme immunoassays to discriminate between infections with herpes simplex virus types 1 and 2. , 1991, Annals of internal medicine.

[5]  E. Monteiro,et al.  Patient attitudes to type specific serological tests in the diagnosis of genital herpes. , 1997, Genitourinary medicine.

[6]  J. Rooney,et al.  Acquisition of genital herpes from an asymptomatic sexual partner. , 1986, The New England journal of medicine.

[7]  A. Nahmias,et al.  Sero-epidemiological and -sociological patterns of herpes simplex virus infection in the world. , 1990, Scandinavian journal of infectious diseases. Supplementum.

[8]  A. Wald,et al.  Genital Herpes in a Primary Care Clinic: Demographic and Sexual Correlates of Herpes Simplex Type 2 Infections , 1997, Sexually transmitted diseases.

[9]  J. Zeh,et al.  Virologic characteristics of subclinical and symptomatic genital herpes infections. , 1995, The New England journal of medicine.

[10]  R L Ashley,et al.  Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera , 1988, Journal of clinical microbiology.

[11]  K. Holmes,et al.  Frequency of Acquisition of First‐episode Genital Infection with Herpes Simplex Virus from Symptomatic and Asymptomatic Source Contacts , 1985, Sexually transmitted diseases.

[12]  Carlos Lopez,et al.  Human Herpesviruses: List of contributors , 2007 .

[13]  A. Nahmias,et al.  The natural history of herpes simplex virus infection of mother and newborn. , 1980, Pediatrics.

[14]  R L Ashley,et al.  The acquisition of herpes simplex virus during pregnancy. , 1997, The New England journal of medicine.

[15]  R. Ashley.  Current concepts in laboratory diagnosis of herpes simplex infection , 1995 .

[16]  K. Holmes,et al.  The Frequency of Unrecognized Type 2 Herpes Simplex Virus Infection Among Women Implications for the Control of Genital Herpes , 1990, Sexually transmitted diseases.

[17]  A. Cunningham,et al.  Herpes simplex virus type 2 antibody in patients attending antenatal or STD clinics , 1993, The Medical journal of Australia.

[18]  S. Safrin,et al.  Comparison of the Western immunoblot assay and a glycoprotein G enzyme immunoassay for detection of serum antibodies to herpes simplex virus type 2 in patients with AIDS , 1992, Journal of clinical microbiology.

[19]  S. Barton,et al.  Asymptomatic shedding and subsequent transmission of genital herpes simplex virus. , 1987, Genitourinary medicine.

[20]  L. Corey,et al.  The management of pregnancies complicated by genital infections with herpes simplex virus. , 1992, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[21]  L. Corey,et al.  Recurrences after oral and genital herpes simplex virus infection. Influence of site of infection and viral type. , 1987, The New England journal of medicine.

[22]  L. Corey,et al.  Development of clinically recognizable genital lesions among women previously identified as having "asymptomatic" herpes simplex virus type 2 infection. , 1989, Annals of internal medicine.