Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation

AbstractBackground and Objective: As Light Emitting Diode (LED) devices are commercially introduced as an alternative for Low Level Laser (LLL) Therapy, the ability of LED in influencing wound healing processes at cellular level was examined. Study Design/Materials and Methods: Cultured fibroblasts were treated in a controlled, randomized manner, during three consecutive days, either with an infrared LLL or with a LED light source emitting several wavelengths (950 nm, 660 nm and 570 nm) and respective power outputs. Treatment duration varied in relation to varying surface energy densities (radiant exposures). Results: Statistical analysis revealed a higher rate of proliferation (p ≤ 0.001) in all irradiated cultures in comparison with the controls. Green light yielded a significantly higher number of cells, than red (p ≤ 0.001) and infrared LED light (p ≤ 0.001) and than the cultures irradiated with the LLL (p ≤ 0.001); the red probe provided a higher increase (p ≤ 0.001) than the infrared LED probe and than the LLL source. Conclusion: LED and LLL irradiation resulted in an increased fibroblast proliferation in vitro. This study therefore postulates possible stimulatory effects on wound healing in vivo at the applied dosimetric parameters.

[1]  T. Mosmann Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[2]  J. Basford Low-energy laser treatment of pain and wounds: hype, hope, or hokum? , 1986, Mayo Clinic proceedings.

[3]  E. McBurney,et al.  The effects of drugs on wound healing: part 1 , 2000, International journal of dermatology.

[4]  J. G. Marshall,et al.  He-Ne laser stimulation of human fibroblast proliferation and attachment in vitro , 1986 .

[5]  C. Enwemeka,et al.  Laser photostimulation accelerates wound healing in diabetic rats , 2001, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[6]  C. Enwemeka,et al.  Laser photostimulation of collagen production in healing rabbit achilles tendons , 1998, Lasers in surgery and medicine.

[7]  J. Kana,et al.  Effect of low-power density laser radiation on healing of open skin wounds in rats. , 1981, Archives of surgery.

[8]  D. Pritchard The effects of light on transdifferentiation and survival of chicken neural retina cells. , 1983, Experimental eye research.

[9]  D A Scudiero,et al.  Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. , 1988, Cancer research.

[10]  M A Pogrel,et al.  Effects of low‐energy gallium‐aluminum‐arsenide laser irradiation on cultured fibroblasts and keratinocytes , 1997, Lasers in surgery and medicine.

[11]  S. McDonough,et al.  Low Intensity laser therapy (830nm) in the management of minor postsurgical wounds: A controlled clinical study , 2001, Lasers in surgery and medicine.

[12]  J C Franquin,et al.  Helium-neon laser treatment transforms fibroblasts into myofibroblasts. , 1990, The American journal of pathology.

[13]  M. Aebi,et al.  The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] Assay Is a Fast and Reliable Method for Colorimetric Determination of Fungal Cell Densities , 1999, Applied and Environmental Microbiology.

[14]  A. J. Nemeth Lasers and wound healing. , 1993, Dermatologic clinics.

[15]  R. Ian Freshney,et al.  Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications , 2010 .

[16]  T. Savunen,et al.  Prediction of wound tensile strength: An experimental study , 1992, The British journal of surgery.

[17]  D. Hirst,et al.  Effect of low intensity monochromatic light therapy (890 nm) on a radiation‐impaired, wound‐healing model in murine skin , 1998, Lasers in surgery and medicine.

[18]  James M. Allen,et al.  Low level laser therapy : current clinical practice in Northern Ireland , 1991 .

[19]  Tiina I. Karu,et al.  The science of low-power laser therapy , 1998 .

[20]  Glenn A. Meyer,et al.  The NASA light-emitting diode medical program—progress in space flight and terrestrial applications , 2000 .

[21]  E. Mester,et al.  The biomedical effects of laser application , 1985, Lasers in surgery and medicine.

[22]  J. Basford,et al.  Low intensity laser therapy: Still not an established clinical tool , 1995, Lasers in surgery and medicine.

[23]  J. Basford,et al.  Does low‐energy helium‐neon laser irradiation alter “in vitro” replication of human fibroblasts? , 1988, Lasers in surgery and medicine.

[24]  Hans H. F. I. van Breugel,et al.  Power density and exposure time of He‐Ne laser irradiation are more important than total energy dose in photo‐biomodulation of human fibroblasts in vitro , 1992, Lasers in surgery and medicine.

[25]  G. Baxter,et al.  Therapeutic lasers : theory and practice , 1995 .

[26]  R. Ian Freshney,et al.  Culture of Animal Cells , 1983 .

[27]  M. Dyson,et al.  Stimulatory effect of 660 nm low level laser energy on hypertrophic scar‐derived fibroblasts: possible mechanisms for increase in cell counts , 1998, Lasers in surgery and medicine.

[28]  P. Kolari,et al.  Comparative effects of exposure to different light sources (He-Ne laser, InGaAl diode laser, a specific type of noncoherent LED) on skin blood flow for the head. , 1996, Acupuncture & electro-therapeutics research.