Multicolor stimulated Raman scattering microscopy

Stimulated Raman scattering (SRS) microscopy has opened up a wide range of biochemical imaging applications by probing a particular Raman-active molecule vibrational mode in the specimen. However, the original implementation with picosecond pulse excitation can only realize rapid chemical mapping with a single Raman band. Here we present a novel SRS microscopic technique using a grating-based pulse shaper for excitation and a grating-based spectrograph for detection to achieve simultaneous multicolor SRS imaging with high sensitivity and high acquisition speeds. In particular, we use a linear combination of the measured CH2 and CH3 stretching signals to map the distributions of protein and lipid contents simultaneously.

[1]  Mortazavi,et al.  Supporting Online Material Materials and Methods Figs. S1 to S13 Tables S1 to S3 References Label-free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy , 2022 .

[2]  Andrew G. Glen,et al.  APPL , 2001 .

[3]  Miss A.O. Penney.  (b) , 1974, The New Yale Book of Quotations.

[4]  Yining Zeng,et al.  Label-free, real-time monitoring of biomass processing with stimulated Raman scattering microscopy. , 2010, Angewandte Chemie.

[5]  Delong Zhang,et al.  Highly Sensitive Vibrational Imaging by Femtosecond Pulse Stimulated Raman Loss. , 2011, The journal of physical chemistry letters.

[6]  Wei Min,et al.  Highly specific label-free molecular imaging with spectrally tailored excitation stimulated Raman scattering (STE-SRS) microscopy. , 2011, Nature photonics.

[7]  S. Albin,et al.  Coupled photonic crystal micro-cavities with ultra-low threshold power for stimulated Raman scattering. , 2011, Optics express.

[8]  Wei Min,et al.  Coherent nonlinear optical imaging: beyond fluorescence microscopy. , 2011, Annual review of physical chemistry.

[9]  Gary D. Noojin,et al.  Stimulated Raman scattering using a single femtosecond oscillator with flexibility for imaging and spectral applications. , 2011, Optics express.

[10]  O. Katz,et al.  Single-pulse stimulated Raman scattering spectroscopy. , 2010, Optics letters.

[11]  Esben Ravn Andresen,et al.  Stimulated Raman scattering microscopy by spectral focusing and fiber-generated soliton as Stokes pulse. , 2011, Optics letters.

[12]  Kazuyoshi Itoh,et al.  Stimulated Raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses. , 2010, Optics express.

[13]  Ji-Xin Cheng,et al.  Vibrational imaging of tablets by epi-detected stimulated Raman scattering microscopy. , 2010, The Analyst.

[14]  Roberta Ramponi,et al.  Fiber-format stimulated-Raman-scattering microscopy from a single laser oscillator. , 2010, Optics letters.

[15]  Yaochun Shen.  Principles of nonlinear optics , 1984 .

[16]  Wei Zheng,et al.  Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser , 2010 .

[17]  Richard H Guy,et al.  Imaging drug delivery to skin with stimulated Raman scattering microscopy. , 2011, Molecular pharmaceutics.

[18]  X. Xie,et al.  Coherent Raman scanning fiber endoscopy. , 2011, Optics letters.

[19]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[20]  Peifang Tian,et al.  Ultrafast measurement of two-photon absorption by loss modulation. , 2002, Optics letters.

[21]  Andrew Folick,et al.  Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy. , 2011, Current opinion in genetics & development.

[22]  Gary D Noojin,et al.  Monitoring stimulated Raman scattering with photoacoustic detection. , 2011, Optics letters.

[23]  X. Xie,et al.  Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering , 2010, Science.

[24]  Dan Fu,et al.  Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. , 2012, Journal of the American Chemical Society.

[25]  Milind Rajadhyaksha,et al.  Rapid confocal imaging of large areas of excised tissue with strip mosaicing. , 2011, Journal of biomedical optics.