Effects of gradual cooling treatment on the skin blackening and physicochemical characteristics of ‘Chuhwang’ pear fruit

‘Chuhwang’ pear fruit was harvested at 187 days after full bloom and stored at 0°C for 70 d after gradual cooling (GC) treatment for 20 d. Direct cooling (DC) treatment was used as a control, and this involved direct storage at 0°C after harvest. Following GC treatment, a weight loss of 4.62% was recorded. In addition. after 30 d of storage at 0°C, the incidence of skin blackening was almost completed and incidence rate in GC treatment significantly decreased to 13.9% compared to 72.2% in DC treatment. Furthermore, in term of GC treatment, the total color differences (ΔE), hardness, and sucrose contents were somewhat lower, but the electrolyte leakage rate and respiration rate were slightly higher than those of DC treatment. Moreover, the contents of hexose, ascorbic acid, and total polyphenol and DPPH radical scavenger activity were slightly higher for the GC treated samples. The activities of polyphenol oxidase and lipoxygenase and thiobarbituric acid reactive substances were low following GC treatment, and no significant differences in the antioxidant enzyme activities were observed between treatment groups. Therefore, these results indicate that the GC treatment prior to cold storage appears to inhibit skin blackening in ‘Chuhwang’ pear fruit by decreasing the chilling sensitivity.

[1]  M. Lv,et al.  Effect of low temperature storage on energy and lipid metabolisms accompanying peel browning of ‘Nanguo’ pears during shelf life , 2018 .

[2]  Maohua Wang,et al.  Effects of Pre-Drying , Delayed Cooling , and Carbon Dioxide on Skin Blackening Disorder in Asian pear ( Pyrus pyrifolia Nakai ) ‘ Chuhwangbae ’ , 2018 .

[3]  J. Viškelis,et al.  Determination of the phenolic composition and antioxidant activity of pear extracts. , 2017 .

[4]  Yu Dong,et al.  Effects of low temperature conditioning on fruit quality and peel browning spot in ‘Huangguan’ pears during cold storage , 2017 .

[5]  Zhengke Zhang,et al.  Low-temperature conditioning induces chilling tolerance in stored mango fruit. , 2017, Food chemistry.

[6]  Yingying Wei,et al.  Sucrose degradation is regulated by 1-methycyclopropene treatment and is related to chilling tolerance in two peach cultivars , 2017 .

[7]  Zhiyong Liu,et al.  Changed activities of enzymes crucial to membrane lipid metabolism accompany pericarp browning in ‘Nanguo’ pears during refrigeration and subsequent shelf life at room temperature , 2016 .

[8]  Jiankang Cao,et al.  Changes in sugar metabolism caused by exogenous oxalic acid related to chilling tolerance of apricot fruit , 2016 .

[9]  Hetong Lin,et al.  The roles of metabolism of membrane lipids and phenolics in hydrogen peroxide-induced pericarp browning of harvested longan fruit , 2016 .

[10]  S. Chakraborty,et al.  Fruit ripening mutants reveal cell metabolism and redox state during ripening , 2015, Protoplasma.

[11]  V. Demidchik.  Mechanisms of oxidative stress in plants: From classical chemistry to cell biology , 2015 .

[12]  최진호,et al.  Effect of Paper Bag Types and Harvesting Dates on Skin Blackening and Fruit Quality of ‘Chuhwangbae’ Pear Trees , 2015 .

[13]  Zhaohui Xue,et al.  Effects of CaCl2 dipping and pullulan coating on the development of brown spot on ‘Huangguan’ pears during cold storage , 2015 .

[14]  E. Lee.  Major Metabolites Involved in Skin Blackening of ???Niitaka??? Pear Stored under Cold Temperature , 2014 .

[15]  Xudong Huang,et al.  Effect of exogenous γ-aminobutyric acid (GABA) treatment on chilling injury and antioxidant capacity in banana peel , 2014 .

[16]  S. Ketsa,et al.  Chilling injury in mango (Mangifera indica) fruit peel: Relationship with ascorbic acid concentrations and antioxidant enzyme activities , 2013 .

[17]  Lihua He,et al.  Maturity and cooling rate affects browning, polyphenol oxidase activity and gene expression of ‘Yali’ pears during storage , 2013 .

[18]  H. Sallanon,et al.  Response of the physiological parameters of mango fruit (transpiration, water relations and antioxidant system) to its light and temperature environment. , 2013, Journal of plant physiology.

[19]  S. Yim,et al.  Effect of Paper Bag Types on the Fruit Quality, Occurrence of Skin Blackening and Comstock Mealybug of ‘Chuhwangbae’ Pear Fruit , 2013 .

[20]  S. Cao,et al.  Sugar metabolism in relation to chilling tolerance of loquat fruit. , 2013, Food chemistry.

[21]  N. Tuteja,et al.  Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. , 2010, Plant physiology and biochemistry : PPB.

[22]  K. Fugate,et al.  Dehydration accelerates respiration in postharvest sugarbeet roots , 2009 .

[23]  Seong-Mo kang,et al.  Antioxidizing Enzyme Activities in Chilling-sensitive and Chilling-tolerant Pepper Fruit as Affected by Stage of Ripeness and Storage Temperature , 2009 .

[24]  Youngsil Han,et al.  A Study of Antioxidative and Hypoglycemic Activities of Omija(Schizandra chinensis Baillon) Extract under Variable Extract Conditions , 2009 .

[25]  J. Bai,et al.  Chilling-induced oxidative stress and antioxidant responses in mume (Prunus mume) fruit during low temperature storage , 2008 .

[26]  M. Ghasemnezhad,et al.  Effect of hot water treatments on chilling injury and heat damage in ‘satsuma’ mandarins: Antioxidant enzymes and vacuolar ATPase, and pyrophosphatase , 2008 .

[27]  Z. Kaniuga.  Chilling response of plants: importance of galactolipase, free fatty acids and free radicals. , 2008, Plant biology.

[28]  Y. Elkind,et al.  The relationship between water loss, lipid content, membrane integrity and LOX activity in ripe pepper fruit after storage , 2006 .

[29]  I. Couée,et al.  Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. , 2006, Journal of experimental botany.

[30]  Joanne L. Simons,et al.  Heat-induced oxidative activity protects suspension-cultured plant cells from low temperature damage. , 2006, Functional plant biology : FPB.

[31]  Lee Yongjae,et al.  Ripening responses and quality changes of 'Fuyu' persimmon fruit as influenced by exogenous ethylene and subsequent short-term storage temperature. , 2006 .

[32]  Y. Elkind,et al.  Characterization of physiological and biochemical factors associated with postharvest water loss in ripe pepper fruit during storage , 2005 .

[33]  G. Lester,et al.  Oxidative Stress: Importance for Postharvest Quality , 2004 .

[34]  배만종,et al.  한국산 배의 저장기간에 따른 polyphenol화합물의 함량 및 성분변화와 항산화효과 , 2003 .

[35]  M. Saltveit,et al.  Effect of chilling on antioxidant enzymes and DPPH‐radical scavenging activity of high‐ and low‐vigour cucumber seedling radicles , 2002 .

[36]  M. T. Lafuente,et al.  Carbohydrates as related to the heat-induced chilling tolerance and respiratory rate of ‘Fortune’ mandarin fruit harvested at different maturity stages , 2002 .

[37]  M. T. Lafuente,et al.  Catalase enzyme activity is related to tolerance of mandarin fruits to chilling , 2000 .

[38]  A. Sturm,et al.  The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning. , 1999, Trends in plant science.

[39]  M. T. Lafuente,et al.  Catalase in the heat-induced chilling tolerance of cold-stored hybrid Fortune mandarin fruits. , 1999, Journal of agricultural and food chemistry.

[40]  R. Guillén-Sans,et al.  The thiobarbituric acid (TBA) reaction in foods: a review. , 1998, Critical reviews in food science and nutrition.

[41]  T. Zor,et al.  Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. , 1996, Analytical biochemistry.

[42]  C. Wang.  Temperature preconditioning affects ascorbate antioxidant system in chilled zucchini squash , 1996 .