Microstructure arrays of DNA using topographic control

DNA is a common biomaterial in nature as well as a good building block for producing useful structures, due to its fine feature size and liquid crystalline phase. Here, we demonstrate that a combination of shear-induced flow and microposts can be used to create various kinds of interesting microstructure DNA arrays. Our facile method provides a platform for forming multi-scale hierarchical orientations of soft- and biomaterials, using a process of simple shearing and controlled evaporation on a patterned substrate. This approach enables potential patterning applications using DNA or other anisotropic biomaterials based on their unique structural characteristics.DNA is a biological building block which has drawn much attention as a structural material for a range of applications. Here, the authors report that DNA structures can be templated using shear-induced flow and microposts for controllable DNA patterning.

[1]  Noel A. Clark,et al.  End-to-End Stacking and Liquid Crystal Condensation of 6– to 20–Base Pair DNA Duplexes , 2007, Science.

[2]  Giyuu Kido,et al.  Annular self‐assembly of DNA molecular chains occurring in natural dry process of diluted solutions , 2005, Biopolymers.

[3]  A. Yodh,et al.  Deposition and drying dynamics of liquid crystal droplets , 2017, Nature Communications.

[4]  P. Gennes.  Wetting: statics and dynamics , 1985 .

[5]  Hyungju Ahn,et al.  Orthogonal Liquid Crystal Alignment Layer: Templating Speed-Dependent Orientation of Chromonic Liquid Crystals. , 2017, ACS applied materials & interfaces.

[6]  K. Saitoh,et al.  Tissue Preservation and Total DNA Extraction form Fish Stored at Ambient Temperature Using Buffers Containing High Concentration of Urea , 1996 .

[7]  Katharina Gaus,et al.  Probing DNA–peptide interaction forces at the single‐molecule level , 2006, Journal of peptide science : an official publication of the European Peptide Society.

[8]  J. Hammond,et al.  Extraction of DNA from preserved animal specimens for use in randomly amplified polymorphic DNA analysis. , 1996, Analytical Biochemistry.

[9]  D. Noréus,et al.  Hydrogenation, purification, and unzipping of carbon nanotubes by reaction with molecular hydrogen: road to graphane nanoribbons. , 2011, ACS nano.

[10]  F. Livolant,et al.  The highly concentrated liquid-crystalline phase of DNA is columnar hexagonal , 1989, Nature.

[11]  Daniel A. Beller,et al.  Elasticity-dependent self-assembly of micro-templated chromonic liquid crystal films. , 2014, Soft matter.

[12]  D. Yoon,et al.  Highly Aligned Plasmonic Gold Nanorods in a DNA Matrix , 2017 .

[13]  O. Lavrentovich,et al.  Elasticity of lyotropic chromonic liquid crystals probed by director reorientation in a magnetic field. , 2012, Physical review letters.

[14]  N. Clark,et al.  Alignment of the columnar liquid crystal phase of nano-DNA by confinement in channels , 2012 .

[15]  M. Sastry,et al.  Linear Superclusters of Colloidal Gold Particles by Electrostatic Assembly on DNA Templates , 2001 .

[16]  James E Hutchison,et al.  Linear assemblies of nanoparticles electrostatically organized on DNA scaffolds , 2003, Nature materials.

[17]  Gerard C L Wong,et al.  Structure and dynamics of liquid crystalline pattern formation in drying droplets of DNA. , 2006, Physical review letters.

[18]  A. Pühler,et al.  Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications. , 2004, Journal of biotechnology.

[19]  Seung‐Hwan Lee,et al.  Relationship between aspect ratio and suspension viscosity of wood cellulose nanofibers , 2014 .

[20]  K. Oh,et al.  In-Plane Switching Mode for Liquid Crystal Displays Using a DNA Alignment Layer. , 2015, ACS applied materials & interfaces.

[21]  D. K. Schwartz,et al.  Macroscopic liquid crystal response to isolated DNA helices. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[22]  Tomiki Ikeda,et al.  Polymer Liquid Crystals , 1997 .

[23]  S. Herminghaus,et al.  Wetting: Statics and dynamics , 1997 .

[24]  Kentaro Tanaka,et al.  A DNA-lipid complex in organic media and formation of an aligned cast film , 1996 .

[25]  Shiho Tokonami,et al.  Highly Ordered Assemblies of Au Nanoparticles Organized on DNA , 2003 .

[26]  D. Yoon,et al.  Control of Periodic Zigzag Structures of DNA by a Simple Shearing Method , 2017, Advanced materials.

[27]  William M. Shih,et al.  Virus-Inspired Membrane Encapsulation of DNA Nanostructures To Achieve In Vivo Stability , 2014, ACS nano.

[28]  Mfi.  Statics and Dynamics , 2014 .

[29]  Shawn M. Douglas,et al.  A Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads , 2012, Science.

[30]  A Bensimon,et al.  Alignment and sensitive detection of DNA by a moving interface. , 1994, Science.

[31]  N. Clark,et al.  Liquid crystal alignment on a chiral surface: interfacial interaction with sheared DNA films. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[32]  Michael W. Davidson,et al.  Multiple liquid crystal phases of DNA at high concentrations , 1988, Nature.

[33]  A. DeSimone,et al.  Corepressor-Directed Preacetylation of Histone H3 in Promoter Chromatin Primes Rapid Transcriptional Switching of Cell-Type-Specific Genes in Yeast , 2010, Molecular and Cellular Biology.