Novel astrocytic protein in multiple sclerosis plaques

Monoclonal antibody J1‐31 (MAb J1‐31, isotype IgG 2b) was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151–157, 1984). In human brain, MAb J1‐31 recognizes an intracellular protein antigen (J1‐31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73‐79, 1986). By immunofluorescence microscopy, MAb J1‐31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Müller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491–502, 1987). In addition, MAb J1‐31 stains ciliated ependymal cells that do not express GFAP.

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