Bioassay-Directed Chemical Analysis of Genotoxic Components in Coastal Sediments

Organic extracts of coastal sediments collected off-shore from Barcelona were submitted to a three-level bioassay-directed chemical fractionation, including gel permeation chromatography (GPC) and normal-phase (NP) and reversed-phase (RP) liquid chromatography (LC). The chemical characterization, directed by the Salmonella microsome mutagenicity assay (TA98+S9, TA98NR+S9, and TA98/1,8DNP+S9), was serried out by capillary GC (CGC) coupled to selective detection systems and by CGC-MS techniques