Antimicrobial and Cytotoxic Activities of Origanum Essential Oils

Three Origanum essential oils, Origanum vulgare ssp. hirtum, Origanum dictamnus, and a commercially available Origanum oil, were analyzed by gas chromatography−mass spectrometry (GC−MS) and showed a high content of carvacrol, thymol, γ-terpinene, and p-cymene representing 73.7%, 92.8%, and 87.78% of the total oil, respectively. The three essential oils exhibited high levels of antimicrobial activity against eight strains of Gram-positive and Gram-negative bacteria. Among the major components of the three oils, carvacrol and thymol exhibited the highest levels of antimicrobial activity, while their biosynthetic precursors γ-terpinene and p-cymene were inactive. The essential oil of O. vulgare ssp. hirtum was extremely bactericidal at 1/4000 dilution and even at dilutions as high as 1/50000 caused considerable decrease in bacterial growth rates. The same essential oil also exhibited high levels of cytotoxicity against four permanent animal cell lines including two derived from human cancers. Keywords: Origa...

[1]  S. Deans,et al.  Antibacterial properties of plant essential oils , 1987 .

[2]  Fred W. McLafferty,et al.  Registry of mass spectral data , 1974 .

[3]  A. F. Afifi,et al.  Effect of Volatile Substances from Species of Labiatae on Rhizospheric and Phyllospheric Fungi of Phaseolus vulgaris , 1975 .

[4]  T. Hayashi,et al.  Antiviral activity of an extract of Cordia salicifolia on herpes simplex virus type 1. , 1990, Planta medica.

[5]  R. Croteau,et al.  Biosynthesis of aromatic monoterpenes: Conversion of γ-terpinene to p-cymene and thymol in Thymus vulgaris L , 1978 .

[6]  V. Gergis,et al.  Antimicrobial activity of essential oils from greek Sideritis species. , 1990, Die Pharmazie.

[7]  M. Tsimidou,et al.  Composition and antioxidant activity of essential oils from Oregano plants grown wild in Greece , 1993 .

[8]  N. Didry,et al.  [Antibacterial activity of thymol, carvacrol and cinnamaldehyde alone or in combination]. , 1993, Die Pharmazie.

[9]  D. Vokou,et al.  Geographic variation of Greek oregano (Origanum vulgare ssp. hirtum) essential oils , 1993 .

[10]  D. Thompson.  Fungitoxic activity of essential oil components on food storage fungi , 1989 .

[11]  T. Lanaras,et al.  Antimicrobial activity of mint essential oils , 1995 .

[12]  G. Flamini,et al.  Composition and antimicrobial properties of essential oils of four Mediterranean Lamiaceae. , 1993, Journal of ethnopharmacology.

[13]  J. Scheffer,et al.  Antimicrobial Activity of Essential Oils: A 1976-1986 Literature Review. Aspects of the Test Methods , 1987, Planta medica.

[14]  D. Vokou,et al.  Carvacrol-rich Plants in Greece , 1989 .

[15]  L. Lam,et al.  Effects of essential oils on glutathione S-transferase activity in mice , 1991 .